Abstract In this thesis, the use of multidisciplinary techniques is proposed as a viable alternative to the current procedures for assessment of footwear, which are costly and time consuming. These techniques are Virtual Reality, Computational Biomechanics, and Artificial Neural Networks. The framework of this research is the virtual assessment of the mechanical comfort in footwear, i.e., comfort pressures in footwear. The main goal of this thesis is to predict the pressures exerted by the shoe over the foot dorsum while walking simulating the contact at this interface. In particular, the Finite Element Method is used to develop a software application for the simulation of the footwear deformation in gait. A first model for the behavior of the shoe upper material is developed. An automatic procedure for the foot-shoe initial alignment is implemented into the application. A methodology to obtain a generic animation of the step for each individual is presented as well as new models for the shoe upper material behavior aimed to improve the developed application. On the other hand, Artificial Neural Networks are successfully applied to predict the force exerted by a sphere that, simulating a bone, pushes a shoe upper material sample. They are also used to predict the pressures exerted by a shoe on the foot dorsum (dorsal pressures) during a complete step. The main contributions of this thesis are: The development of an innovative simulator that will allow footwear manufacturers to perform virtual evaluations of the functional and esthetic features of their designs without having to construct the real prototype, and the development of another innovative tool for the prediction of the dorsal pressures exerted by the footwear while walking.