ABSTRACT Coastal and estuarine waters are notable for the high spatial and temporal variability in their hydrological, physical and chemical properties. This variability is reflected in the complex dynamics of their phytoplankton community. In addition, coastal areas are subject to high anthropogenic pressure, the main effect of which is an increase in nutrient loads from terrestrial origin to receiving waters, where they can cause eutrophication problems. The aim of this thesis is to characterize the spatial variability of the phytoplankton communities in coastal and transitional waters which receive nutrient inputs from various sources. Obtaining information about the functioning of these ecosystems is required so that the right management measures can be adopted to improve the water quality. In this thesis, the concentrations of photosynthetic pigments in coastal water samples were measured using high-performance liquid chromatography. This data was then entered into the CHEMTAX software to obtain the taxonomic composition of the phytoplankton. Compared to microscopy, the main advantages of this technique are lower cost, more rapid analyses and greater precision. Furthermore, an initial attempt is made to estimate chlorophyll a levels, which are an indicator of phytoplankton biomass, by using high spatial resolution satellite images. The main advantage of this technique is that it provides a synoptic view not attainable with traditional monitoring. The characteristics of both these techniques make them suitable for the monitoring requirements of the Water Framework Directive. The results of this thesis indicate that the main problems of water quality are found in areas with reduced exchange and dilution with the sea. In this thesis, these areas are represented by Gandia Harbour and an area near the Serpis river mouth. The need to control illegal waste water discharges and to ensure the proper implementation of the existing agricultural best management practices, are among the most important management recommendations to improve the state of these systems.