RESUMEN EN INGLES The development of the estimation observers of variables or parameters in a biotechnological process is the main topic in the present work. It talks about the development of a state observer that considers in its design the uncertainty of the model in the system, which are defined inside of the bidimensional polygon or tridimensional polyhedral sides that limit the operation of this system. This development shows itself in two facets. The first one, is the application of a concept to a Luenberger classic observer, which has as its main characteristic that the gain matrix is determined by following the procedure of linear matrix inequality (LMI). The second facet is about a hybrid observer that is made up by a Luenberger observer developed like the previous one I have mentioned, and also by a curved observer that helps the observer transit freely among other observers by a parameter. Besides, it will develop an extension of the hybrid observer to be applied to a multifrequency scheme, defined by available linear measures and measures out of the line and delayed. These theories can be compared to a classic asymptotic observer and an extended Kalman filter, showing results under the same simulation conditions.