The Agromyzidae family includes the most important dipterous species miners of crops for agriculture. Knowledge of 2900 worldwide species are cited, of which about 7% are considered of agronomic interest. Despite the high specificity that has Agromyzidae by their host-plants, it is noted that in recent decades there has been a significant increase of the oligophagy and polyphagy of many species due to the constant change produced in the ecosystems and the increase of the number of intensive crops.

      Knowledge of the Agromyzidae biodiversity is one of the main factors to be considered under the control of present and future pests. As result of a systematic biodiversity study in the three parks of Eastern Spain: “Tinença de Benifassà” (Castellón), “Font Roja” (Alicante) and “Lagunas de La Mata-Torrevieja” (Alicante) new faunistic data included in 6 Agromyzidae genera are presented. Thirteen new species collected with Malaise trap are recorded for the first time in Spain: Agromyza anthracina Meigen, 1830; A. bromi Spencer, 1966; A. hiemalis Becker, 1908; A. megalopsis Hering, 1933; Aulagromyza luteoscutellata (de Meijere, 1924); Au. similis (Brischke, 1880); Au. trivitatta (Loew, 1873); Liriomyza graminivora Hering, 1949; Melanagromyza eupatorii Spencer, 1957; M. spinulosa Spencer, 1974; Phytobia cerasiferae (Kangas, 1955); Ph. lunulata (Hendel, 1920) and Pseudonapomyza palliditarsis Cerny, 1992. General information about the host-plants and geographical distribution are included. 

      A new Agromyzidae pest is reported in Spain, Melanagromyza sojae (Zehnter, 1900). The stem-miner was captured with Malaise trap in “Tinença de Benifassà”. Information is given about distribution, damage, control, host-plants, biology and ecology of this pest. Identification rules are indicated by its distinction from the rest of Agromyzidae miners on soybean in Europe. 

      Pseudonapomyza atratula Zlobin, 2002 a species earlier known only in Tunisia is reported for the first time in Europe (Spain). Their host-plants, Avena barbata Pott ex Link and A. fatua L. are indicated here for the first time. Ps. atratula belongs to the same group of species as Ps. atra  (Meigen, 1830). Morphological criteria are given to separate these two closely connected species, in particular on the genitalia of the males. Some biological and phenological elements are also given for Ps. atratula. 

      Five new species for Pseudonapomyza are described: Ps. curvata n. sp; Ps. benifassae n. sp.; Ps. longitata n. sp.; Ps. mediterranea n. sp. and Ps. sicicornis n. sp. In addition, nineteen new species for science belonging to Agromyza, Cerodontha, Liriomyza, Metopomyza, Ophiomyia and Phytomyza genera are reported to be considered into Agromyzidae fauna.

      A tool for the ecological studies of habitats is their biodiversity analysis. The biodiversity alpha, beta, and gamma for each one of the Natural Parks are studied with both, biodiversity indexes and captures approximation to different statistical models.

      This study presents 34 new host-plants for Agromyzidae from a total of 153 interactions established in 94 genera belonging to 27 botanical families. The interactions were established in three Natural Parks from Eastern Spain on 27 Agromyzidae species: Ophiomyia beckeri (Hendel, 1923); O. ononidis Spencer, 1966; Amauromyza (Amauromyza) balcanica (Hendel, 1931); A. (Amauromyza) carlinae (Hering, 1944); A. (Amauromyza) morionella (Zetterstedt, 1848); A. (Cephalomyza) flavifrons (Meigen, 1830); A. (Cephalomyza) karli (Hendel, 1927); Chromatomyia horticola (Goureau, 1851); Chr.  periclymeni (Hendel, 1922); Liriomyza brassicae (Riley, 1884); L. bryoniae (Kaltenbach, 1858); L. cicerina (Rondani, 1875); L. congesta (Becker, 1903); L. dianthicola (Venturi, 1949); L. orbona (Meigen, 1830); L. pascuum (Meigen, 1838); L. strigata (Meigen, 1830); L. trifolii (Burgess in Comstock, 1880); Napomyza lateralis (Fallén, 1823); Phytomyza hellebori Kaltenbach, 1872; P. plantaginis Robineau-Desvoidy, 1851; Pseudonapomyza atratula Zlobin, 2002; and five species belonging to Liriomyza, Phytomyza and Pseudonapomyza genera. Agromyzidae damages on families and botanical genera are studied and broken down in function of botanical species and their miners. 

      Pseudonapomyza is one of the leaf-miners genera of monocots in the southeast area of Spain. Knowledge of the population fluctuation of these Agromyzidae miners is key to the establishment of control measures. It shows the close relationship between fluctuating temperatures and the miners evolution through the use of multivariate statistical tools. Pseudonapomyza captures were studied throughout three years of sampling with Malaise trap. It is noted that 82% of the differences in captures are due to the Pseudonapomyza bioecology within each park, being essentially the temperature the factor that explains the 79% of the population dynamics in the areas studied.