SUMMARY
Those chemical pollutants that are regulated represent but a small fraction of the universe of chemicals that occur in the environment as a result of both human activity and natural processes. Due to the extensive and increasing number of known and unknown pollutants, there is a need for screening methods in environmental monitoring.
Immunochemistry has proven to be an interesting option for residue monitoring and control in clinical, food and environmental analysis. This methodology offers a range of possibilities for matching different analytical needs. The binding properties of an antibody (Ab) to an antigen (Ag) have been used for the development of a wide variety of analytical techniques applicable in rapid environmental control.
A rapid immunosensing methodology that employs the so called homogeneous-heterogeneous assay mode is presented. The immunosensor is based on the homogeneous competition among the analyte, a fluorescent tracer, and the antibody, followed by separation of free and bound species by means of a restricted access alkyl-diol silica C18 reversed-phase chromatographic support. In order to develop a general labeling methodology, fluorescent tracers are synthesized from oligonucleotides covalently bound to the hapten in 3' position and the marker in 5'. Organic solvents 2-propanol and acetonitrile up to 15% (v/v) are well tolerated, while methanol can be added to 4%. The improvement of sensitivity was assayed by employing a multiple-label tracer and The immunosensor principle is demonstrated by determining atrazine and sufathiazole in natural waters.

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Objectius


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