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Browsing by Author "Navarro Bohigues, José Antonio"

Now showing items 1-20 of 27

A conserved motif in three viral movement proteins from different genera is required for host factor recruitment and cell-to-cell movement

NAVARRO BOHIGUES, JOSE ANTONIO; Serra-Soriano, Marta; Corachán Valencia, Lorena; Pallás Benet, Vicente (Nature Publishing Group, 2020-03-16)

[EN] Due to their minimal genomes, plant viruses are forced to hijack specific cellular pathways to ensure host colonization, a condition that most frequently involves physical interaction between viral and host proteins. ...
A model for transport of a viral membrane protein through the early secretory pathway: minimal sequence and endoplasmic reticulum lateral mobility requirements

Serra Soriano, Marta; Pallás Benet, Vicente; Navarro Bohigues, José Antonio (Wiley, 2014-03)

[EN] Viral movement proteins exploit host endomembranes and the cytoskeleton to move within the cell via routes that, in some cases, are dependent on the secretory pathway. For example, melon necrotic spot virus p7B, a ...
A potyvirus vector efficiently targets recombinant proteins to chloroplasts, mitochondria and nuclei in plant cells when expressed at the amino terminus of the polyprotein

Majer, Eszter; Navarro Bohigues, José Antonio; Daros Arnau, Jose Antonio (Wiley-VCH Verlag, 2015-11)

Plant virus-based expression systems allow quick and efficient production of recombinant proteins in plant biofactories. Among them, a system derived from tobacco etch virus (TEV; genus potyvirus) permits coexpression of ...
A sensitive and rapid RNA silencing suppressor activity assay based on alfalfa mosaic virus expression vector

Martínez-Pérez, Mireya; NAVARRO BOHIGUES, JOSE ANTONIO; Pallás Benet, Vicente; SANCHEZ NAVARRO, JESUS ANGEL (Elsevier, 2019-10-15)

[EN] Plant viruses express RNA silencing suppressor (RSS) proteins to counteract plant defence mechanisms. Here, we describe a method to assess the RSS activity based on an alfalfa mosaic virus (AMV) RNA 3 expression vector ...
An Update on the Intracellular and Intercellular Trafficking of Carmoviruses

NAVARRO BOHIGUES, JOSE ANTONIO; Pallás Benet, Vicente (Frontiers Media SA, 2017-10-18)

[EN] Despite harboring the smallest genomes among plant RNA viruses, carmoviruses have emerged as an ideal model system for studying essential steps of the viral cycle including intracellular and intercellular trafficking. ...
Análisis funcional y localización subcelular de las proteínas implicadas en el movimiento intra e intercelular del virus de las manchas necróticas del melón (MNSV)

Genovés Martínez, Ainhoa (Universitat Politècnica de València, 2008-06-27)

Los virus de plantas constituyen, en ocasiones, una seria amenaza para numerosos cultivos. A pesar de las numerosas investigaciones realizadas, existe todavía un gran desconocimiento de las rutas y los mecanismos que operan ...
Association between flower stalk elongation, an Arabidopsis developmental trait, and the subcellular location and movement dynamics of the viral non-structural protein P3.

López-González, Silvia; NAVARRO BOHIGUES, JOSE ANTONIO; Pacios, Luis F.; Sardaru, Papaiah; Pallás Benet, Vicente; Sánchez, Flora; Ponz, Fernando (Blackwell Publishing, 2020-10)

[EN] Virus infections affect plant developmental traits but this aspect of the interaction has not been extensively studied so far. Two strains ofTurnip mosaic virusdifferentially affectArabidopsisdevelopment, especially ...
Caracterización del determinante viral que confiere la capacidad de infectar Arabidopsis thaliana al virus del arrugamiento del nabo

Sáiz Bonilla, María (Universitat Politècnica de València, 2019-04-01)

[ES] Actualmente, los carmovirus se clasifican en tres géneros diferentes, en base a las relaciones filogenéticas de sus RNA polimerasas (RdRp): los Alfacarmovirus, cuyo miembro tipo es el virus del moteado del clavel, ...
Comparative analysis among the small RNA populations of source, sink and conductive tissues in two different plant-virus pathosystems

Herranz Gordo, Maria Del Carmen; Navarro Bohigues, José Antonio; Sommen, Evelien; Pallás Benet, Vicente (BioMed Central, 2015-02-22)

Conclusions: We compare for the first time the sRNA profile of four different tissues, including source, sink and conductive (phloem) tissues, in two plant-virus pathosystems. Our results indicate that antiviral silencing ...
Comparative proteomic analysis of melon phloem exudates in response to viral infection

Serra Soriano, Marta; Navarro Bohigues, José Antonio; Genovés Martínez, Ainhoa; Pallás Benet, Vicente (Elsevier, 2015-06-21)

Despite the importance of phloem as an integrative pathway for resource distribution, signaling and plant virus transport little is known about the modifications induced by these pathogens in phloem sap proteome. Only one ...
Contribution of topology determinants of a viral movement protein on its membrane association, intracellular traffic and viral cell-to-cell movement

Genovés Martínez, Ainhoa; Pallás Benet, Vicente; Navarro Bohigues, José Antonio (American Society for Microbiology, 2011)

[EN] The p7B movement protein (MP) of Melon necrotic spot virus (MNSV) is a single-pass membrane protein associated with the endoplasmic reticulum (ER), the Golgi apparatus (GA), and plasmodesmata (Pd). Experimental data ...
Cucurbit chlorotic yellows virus p22 suppressor of RNA silencing binds single-, double-stranded long and short interfering RNA molecules in vitro

Salavert, Ferran; NAVARRO BOHIGUES, JOSE ANTONIO; Owen, Carolyn A.; Khechmar, Souheyla; Pallás Benet, Vicente; Livieratos, Ioannis C. (Elsevier, 2020-04-02)

[EN] Cucurbit chlorotic yellows virus (CCYV) is a new member of the genus Crinivirus (family Closteroviridae) with a bi-partite genome. CCYV RNA 1-encoded p22 has recently been reported to be a weak local suppressor of RNA ...
Deciphering the intracellular dual targeting of the melon necrotic spot virus coat protein, its interaction with host factors and their roles in plant defense

Sáiz Bonilla, María (Universitat Politècnica de València, 2023-09-01)

[ES] Los virus de plantas son los agentes causales de un gran número de enfermedades en plantas que ocasionan grandes pérdidas económicas. El virus de las manchas necróticas del melón (MNSV), es un pequeño virus de RNA ...
Dissecting the multifunctional role of the N-terminal disordered domain of a plant virus coat protein in RNA packaging, viral movement and interference with antiviral plant defense

Serra Soriano, Marta; Navarro Bohigues, José Antonio; Pallás Benet, Vicente (Blackwell Publishing, 2017)

[EN] The coat protein (CP) of Melon necrotic spot virus (MNSV) is structurally composed of three major domains. The middle S-domain builds a robust protein shell around the viral genome, whereas the C-terminal protruding ...
Hexanoic Acid Treatment Prevents Systemic MNSV Movement in Cucumis melo Plants by Priming Callose Deposition Correlating SA and OPDA Accumulation

Fernandez-Crespo, E.; NAVARRO BOHIGUES, JOSE ANTONIO; Serra Soriano, Marta; Finiti, I.; García Agustín, Pilar; Pallás Benet, Vicente; Gonzalez-Bosch, C. (Frontiers Media SA, 2017-10-20)

[EN] Unlike fungal and bacterial diseases, no direct method is available to control viral diseases. The use of resistance-inducing compounds can be an alternative strategy for plant viruses. Here we studied the basal ...
Highly efficient construction of infectious viroid-derived clones

Márquez-Molins, Joan; NAVARRO BOHIGUES, JOSE ANTONIO; Pallás Benet, Vicente; Gomez, Gustavo Germán (Springer (Biomed Central Ltd.), 2019-08-01)

[EN] Background Viroid research generally relies on infectious cDNA clones that consist of dimers of the entire viroid sequence. At present, those dimers are generated by self-ligation of monomeric cDNA, a strategy that ...
ICTV Virus Taxonomy Profile: Ophioviridae

Garcia, M.L; Dal Bo, Elena; da Graca, John V.; Gago Zachert, Selma Persida; Hammond, J; Moreno, Pedro; Natsuaki, T; Pallás Benet, Vicente; Navarro Bohigues, José Antonio; Reyes, Carina; Luna, Gabriel Robles; Sasaya, Takahide; Tzanetakis, Loannis E.; Vaira, Anna Maria; Verbeek, Martin (Microbiology Society, 2017)

[EN] The Ophioviridae is a family of filamentous plant viruses, with single-stranded negative, and possibly ambisense, RNA genomes of 11.3-12.5 kb divided into 3-4 segments, each encapsidated separately. Virions are naked ...
Identificación de los receptores de las translocasas de proteínas de mitocondrias y cloroplastos implicadas en el transporte dual de la proteína de cubierta del virus de las manchas necróticas del melón

Martín Merchán, Andrea (Universitat Politècnica de València, 2020-02-12)

[ES] El transporte específico de proteínas a mitocondrias o cloroplastos se realiza por medio de dos complejos de canales proteicos o translocasas ubicados en las membranas externa (TOM/TOC) e interna (TIM/TIC). Aunque no ...
In memoriam of Ricardo Flores: The career, achievements, and legacy of an inspirational plant virologist

Pallás Benet, Vicente; HERNANDEZ FORT, CARMEN; Marcos, Jose F.; Daròs, José-Antonio; Ambrós, Silvia; Navarro, Beatriz; NAVARRO BOHIGUES, JOSE ANTONIO; La Peña Del Rivero, Marcos De; Gago-Zachert, Selma; Gas, Maria E.; CARBONELL, ALBERTO; López Del Rincón, Carmelo; Martínez-De Alba, Angel E.; Di Serio, Francesco; Moreno, Pedro (Elsevier, 2022-04-15)

[EN] Ricardo Flores (1947-2020) focused his research on the identification, replication, pathogenesis, and evolution of viroids, the minimal non-protein-coding circular RNAs (250-400 nt) able to replicate and incite diseases ...
Key checkpoints in the movement of plant viruses through the host

NAVARRO BOHIGUES, JOSE ANTONIO; SANCHEZ NAVARRO, JESUS ANGEL; Pallás Benet, Vicente (Academic Press, 2019-07-18)

[EN] Plant viruses cannot exploit any of the membrane fusion-based routes of entry described for animal viruses. In addition, one of the distinctive structures of plant cells, the cell wall, acts as the first barrier against ...