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Browsing by Author "Vázquez Vilar, Marta"

Now showing items 1-12 of 12

A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard

Vázquez-Vilar, Marta; Bernabé-Orts, Joan Miquel; Fernández Del Carmen, María Asunción; Ziarsolo Areitioaurtena, Pello; Blanca Postigo, José Miguel; Granell Richart, Antonio; Orzáez Calatayud, Diego Vicente (Springer (Biomed Central Ltd.), 2016)

[EN] Background: The efficiency, versatility and multiplexing capacity of RNA-guided genome engineering using the CRISPR/Cas9 technology enables a variety of applications in plants, ranging from gene editing to the ...
Anti-CRISPR protein AcrIIA4 as a novel tool to control CRISPR/Cas9-mediated gene editing and activation in Nicotiana benthamiana

Calvache Román, Camilo Alberto (Universitat Politècnica de València, 2021-02-12)

[ES] La tecnología CRISPR-Cas9 se ha convertido en una herramienta esencial en la biotecnología de plantas, cambiando nuestra capacidad para manipular genomas y estudiar redes moleculares gracias a las posibilidades ofrecidas ...
Custom-made design of metabolite composition in N. benthamiana leaves using CRISPR activators

Selma, Sara; Sanmartín, Neus; Espinosa-Ruiz, Ana; Gianoglio, Silvia; López-Gresa, María Pilar; Vázquez-Vilar, Marta; Flors, Victor; GRANELL RICHART, ANTONIO; Orzáez Calatayud, Diego Vicente (Blackwell Publishing, 2022-08)

[EN] Transcriptional regulators based on CRISPR architecture expand our ability to reprogramme endogenous gene expression in plants. One of their potential applications is the customization of plant metabolome through the ...
DESIGN OF GENETIC ELEMENTS AND SOFTWARE TOOLS FOR PLANT SYNTHETIC BIOLOGY

Vázquez Vilar, Marta (Universitat Politècnica de València, 2016-09-01)

[EN] Synthetic Biology is an emerging interdisciplinary field that aims to apply the engineering principles of modularity, abstraction and standardization to genetic engineering. The nascent branch of Synthetic Biology ...
DNA assembly standards: Setting the low-level programming code for plant biotechnology

Vázquez-Vilar, Marta; Orzáez Calatayud, Diego Vicente; Patron, N (Elsevier, 2018)

[EN] Synthetic Biology is defined as the application of engineering principles to biology. It aims to increase the speed, ease and predictability with which desirable changes and novel traits can be conferred to living ...
Efficient Cas9 multiplex editing using unspaced sgRNA arrays engineering in a Potato virus X vector

Uranga-Ruiz De Eguino, Mireia; Aragones, V; Selma García, Sara; Vázquez-Vilar, Marta; Orzáez Calatayud, Diego Vicente; Daròs, José-Antonio (Blackwell Publishing, 2021-04)

[EN] Systems based on the clustered, regularly interspaced, short palindromic repeat (CRISPR) and CRISPR-associated proteins (Cas) have revolutionized genome editing in many organisms, including plants. Most CRISPR-Cas ...
Estudio de diversos parámetros que afectan la edición genómica mediada por CRISPR-Cas12a/Cas9 en Nicotiana benthamiana

Sánchez Vicente, Javier (Universitat Politècnica de València, 2020-02-12)

[ES] En los últimos años, la tecnología CRISPR ha revolucionado la ingeniería del genoma en las plantas. El sistema CRISPR / Cas ofrece una alternativa para la generación de rupturas de doble cadena de ADN (DSB) en un lugar ...
Estudio del editado de genoma de precisión en Nicotiana benthamiana mediante el sistema prime editing

Soriano Revert, Antonio (Universitat Politècnica de València, 2020-07-11)

[ES] Aunque mucha gente no es consciente de ello, la mejora genética de plantas ha sido aplicada desde los inicios de la agricultura. Desde siempre, se ha establecido una selección artificial, bien sea por la simple elección ...
Implementation of CRISPR-based gene targeting tools for genome engineering in Plant Synthetic Biology

Climent Catalá, Alicia (Universitat Politècnica de València, 2018-09-19)

[EN] Synthetic Biology is a novel interdisciplinary field that aims to re-design existing natural biological systems or even to create new ones from an engineering perspective. Modularity, standardization, abstraction, ...
Multigene Engineering by GoldenBraid Cloning: From Plants to Filamentous Fungi and Beyond

Vázquez-Vilar, Marta; Gandía, Mónica; García-Carpintero, Victor; Marqués, Eric; Sarrion-Perdigones, Alejandro; Yenush, Lynne; Polaina, Julio; Manzanares, Paloma; Marcos, Jose F.; Orzáez Calatayud, Diego Vicente (Wiley, 2020-03-09)

[EN] Many synthetic biologists have adopted methods based on Type IIS restriction enzymes and Golden Gate technology in their cloning procedures, as these enable the combinatorial assembly of modular elements in a very ...
Puesta a punto de un sistema de evolución dirigida en plantas mediada por CRISPR.

Hoyas Sánchez, Eva (Universitat Politècnica de València, 2023-02-14)

[ES] La posibilidad de generar variantes proteicas artificiales es una estrategia que está revolucionando nuestra capacidad para desarrollar funciones nuevas o mejoradas para muchas aplicaciones en biología y biotecnología. ...
Strong and tunable anti-CRISPR/Cas activities in plants

Calvache-Román, Camilo Alberto; Selma, Sara; Vázquez-Vilar, Marta; Uranga, Mireia; Fernández-Del-Carmen, Asun; Daròs, José-Antonio; Orzáez Calatayud, Diego Vicente (Blackwell Publishing, 2022-02)

[EN] CRISPR/Cas has revolutionized genome engineering in plants. However, the use of anti-CRISPR proteins as tools to prevent CRISPR/Cas-mediated gene editing and gene activation in plants has not been explored yet. This ...