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Listar por autor "Orzáez Calatayud, Diego Vicente"

Mostrando ítems 1-20 de 71

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A coat-independent superinfection exclusion rapidly imposed in Nicotiana benthamiana cells by tobacco mosaic virus is not prevented by depletion of the movement protein

Julve Parreño, Jose Manuel; Gandia Fernàndez, Antoni; Fernández Del Carmen, María Asunción; Sarrion-Perdigones, Alejandro; Castelijns, Bas; Granell Richart, Antonio; Orzáez Calatayud, Diego Vicente (Springer Verlag (Germany), 2013-04)

[EN] New evidence is emerging which indicates that population variants in plant virus infections are not uniformly distributed along the plant, but structured in a mosaic-like pattern due to limitation to the superinfection ...
A copper switch for inducing CRISPR/Cas9-based transcriptional activation tightly regulates gene expression in Nicotiana benthamiana

García-Pérez, Elena; Diego-Martín, Borja; Quijano-Rubio, Alfredo; Moreno-Giménez, Elena; Selma, Sara; Orzáez Calatayud, Diego Vicente; Vázquez-Vilar, Marta (Springer (Biomed Central Ltd.), 2022-03-24)

[EN] Background CRISPR-based programmable transcriptional activators (PTAs) are used in plants for rewiring gene networks. Better tuning of their activity in a time and dose-dependent manner should allow precise control ...
A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard

Vázquez-Vilar, Marta; Bernabé-Orts, Joan Miquel; Fernández Del Carmen, María Asunción; Ziarsolo Areitioaurtena, Pello; Blanca Postigo, José Miguel; Granell Richart, Antonio; Orzáez Calatayud, Diego Vicente (Springer (Biomed Central Ltd.), 2016)

[EN] Background: The efficiency, versatility and multiplexing capacity of RNA-guided genome engineering using the CRISPR/Cas9 technology enables a variety of applications in plants, ranging from gene editing to the ...
A multilevel, developmentally controlled gene engineering strategy for tomato fortification and protection

Cocaliadis Caisson, María Florencia (Universitat Politècnica de València, 2017-11-03)

Plastids are the cellular organelles where many of the visual, health and flavor-related metabolites are produced and stored in the fruit, and therefore are valuable components for consumers and breeders. The more sugar ...
A synthetic biology approach for consistent production of plant-made recombinant polyclonal antibodies against snake venom toxins

Julve Parreño, Jose Manuel; Huet, Estefania; Fernandez-del-Carmen, Asun; Segura, Alvaro; Venturi, Micol; Gandia, Antoni; Pan, Wei-song; Albaladejo, Irene; Forment Millet, José Javier; Pla, Davinia; Wigdorovitz, Andrés; Calvete, Juan J.; Gutierrez, Carlos; Gutiérrez, José María; GRANELL RICHART, ANTONIO; Orzáez Calatayud, Diego Vicente (Blackwell Publishing, 2018-03)

[EN] Antivenoms developed from the plasma of hyperimmunized animals are the only effective treatment available against snakebite envenomation but shortage of supply contributes to the high morbidity and mortality toll of ...
A VIN1 GUS::GFP fusion reveals activated sucrose metabolism occurring in interspersed cells during tomato fruit ripening

Hueso Estornell, Leandro; Pons Puig, Clara; Martinez, Alicia; O’Connor, José Enrique; Orzáez Calatayud, Diego Vicente; Granell Richart, Antonio (Elsevier, 2013-08-15)

[EN] The tomato is a model for fleshy fruit development and ripening. Here we report on the identification of a novel unique cell autonomous/cellular pattern of expression that was detected in fruits of transgenic tomato ...
An efficient method for medium throughput screening of cuticular wax composition in different plant species

Fernández Moreno, Josefina Patricia; Malitsky, S; Lashbrooke, J; Biswal, Ajaya Kumar; Racovita, Radu C.; Mellerowicz, Ewa J.; Jetter, Reinhard; Orzáez Calatayud, Diego Vicente; Aharoni, A; Granell Richart, Antonio (Springer Verlag (Germany), 2016-04)

[EN] Introduction Most aerial plant organs are covered by a cuticle, which largely consists of cutin and wax. Cuticular waxes are mixtures of dozens of compounds, mostly very-long-chain aliphatics that are easily extracted ...
Anthocyanins double the shelf life of tomatoes by delaying over-ripening and reducing susceptibility to grey mould

Zhang, Y; BUTELLI, E; De Stefano, R; Schoonbeek, HJ; Magusin, A; Pagliarani, C; Wellner, N; Hill, L; Orzáez Calatayud, Diego Vicente; Granell Richart, Antonio; JONES, J; Martin, C (Elsevier (Cell Press), 2013-06-17)

[EN] Shelf life is an important quality trait for many fruit, including tomatoes. We report that enrichment of anthocyanin, a natural pigment, in tomatoes can significantly extend shelf life. Processes late in ripening ...
Anti-CRISPR protein AcrIIA4 as a novel tool to control CRISPR/Cas9-mediated gene editing and activation in Nicotiana benthamiana

Calvache Román, Camilo Alberto (Universitat Politècnica de València, 2021-02-12)

[ES] La tecnología CRISPR-Cas9 se ha convertido en una herramienta esencial en la biotecnología de plantas, cambiando nuestra capacidad para manipular genomas y estudiar redes moleculares gracias a las posibilidades ofrecidas ...
Biomanufacturing of protective antibodies and other therapeutics in edible plant tissues for oral applications

Juarez, Paloma; Virdi, Vikram; Depicker, Ann; Orzáez Calatayud, Diego Vicente (Wiley Open Access, 2016-09)

[EN] Although plant expression systems used for production of therapeutic proteins have the advantage of being scalable at a low price, the downstream processing necessary to obtain pure therapeutic molecules is as expensive ...
Characterization of a New Pink-Fruited Tomato Mutant Results in the Identification of a Null Allele of the SlMYB12 Transcription Factor

Fernández Moreno, Josefina Patricia; Tzfadia, Oren; Forment Millet, José Javier; Presa Castro, Silvia; Rogachev, Ilana; Meir, Sagit; Orzáez Calatayud, Diego Vicente; Aharoni, Aspah; Granell Richart, Antonio (American Society of Plant Biologists, 2016)

[EN] The identification and characterization of new tomato (Solanum lycopersicum) mutants affected in fruit pigmentation and nutritional content can provide valuable insights into the underlying biology, as well as a source ...
Combinatorial Analysis of Secretory Immunoglobulin A (sIgA) Expression in Plants

Juárez Ortega, Paloma; Huet Trujillo, Estefanía; Sarrion-Perdigones, Alejandro; Falconi, E.E.; Granell Richart, Antonio; Orzáez Calatayud, Diego Vicente (MDPI, 2013-03)

Delivery of secretory immunoglobulin A (sIgA) to mucosal surfaces as a passive immunotherapy agent is a promising strategy to prevent infectious diseases. Recombinant sIgA production in plants requires the co-expression ...
Construcción de un nuevo interruptor genético basado en recombinación especifíca de sitio para Biología Sintética de Plantas

Quijano Rubio, Alfredo (Universitat Politècnica de València, 2015-09-07)

[ES] La Biología Sintética de Plantas es una disciplina emergente que propone el empleo de los principios de ingeniería para desarrollar nuevas funciones en organismos vegetales, tales como la introducción de una nueva ...
Creación de una aplicación web en el contexto de la competición IGEM 2016

Portolés Morales, Manuel Guillermo (Universitat Politècnica de València, 2017-01-04)

[ES] La competición IGEM es un evento anual organizado por el MIT que versa sobre Biología Sintética y en el que participan institutos y universidades a nivel internacional. El objetivo es que los participantes planteen ...
CRISPR-Cas12a genome editing at the whole-plant level using two compatible RNA virus vectors

Uranga, Mireia; Vázquez-Vilar, Marta; Orzáez Calatayud, Diego Vicente; Daròs, José-Antonio (Mary Ann Liebert Inc., 2021-10-15)

[EN] The use of viral vectors that can replicate and move systemically through the host plant to deliver bacterial CRISPR components enables genome editing at the whole-plant level and avoids the requirement for labor-intensive ...
Custom-made design of metabolite composition in N. benthamiana leaves using CRISPR activators

Selma, Sara; Sanmartín, Neus; Espinosa-Ruiz, Ana; Gianoglio, Silvia; López-Gresa, María Pilar; Vázquez-Vilar, Marta; Flors, Victor; GRANELL RICHART, ANTONIO; Orzáez Calatayud, Diego Vicente (Blackwell Publishing, 2022-08)

[EN] Transcriptional regulators based on CRISPR architecture expand our ability to reprogramme endogenous gene expression in plants. One of their potential applications is the customization of plant metabolome through the ...
Design and Construction of Multigenic Constructs for Plant Biotechnology Using the GoldenBraid Cloning Strategy

Sarrion-Perdigones, Alejandro; Palací, Jorge; Granell Richart, Antonio; Orzáez Calatayud, Diego Vicente (Humana Press, 2014)

GoldenBraid (GB) is an iterative and standardized DNA assembling system specially designed for Multigene Engineering in Plant Synthetic Biology. GB is based on restriction–ligation reactions using type IIS restriction ...
Design and development of modular DNA assembly tools for Multigene Engineering and Synthetic Biology in Plants

Sarrión Perdigones, Manuel Alejandro (Universitat Politècnica de València, 2014-02-07)

The post-genomics era has put at the disposal of modern plant breeders an endless list of genetic building blocks for the design of new biotechnological crops. After a first wave of single-gene transgenic with controversial ...
DESIGN OF GENETIC ELEMENTS AND SOFTWARE TOOLS FOR PLANT SYNTHETIC BIOLOGY

Vázquez Vilar, Marta (Universitat Politècnica de València, 2016-09-01)

[EN] Synthetic Biology is an emerging interdisciplinary field that aims to apply the engineering principles of modularity, abstraction and standardization to genetic engineering. The nascent branch of Synthetic Biology ...
Development and characterization of two new tools for plant genetic engineering: A CRISPR/Cas12a-based mutagenesis system and a PhiC31-based gene switch

Bernabé Orts, Juan Miguel (Universitat Politècnica de València, 2019-12-16)

[ES] La mejora genética vegetal tiene como objetivo la obtención de plantas con rasgos mejorados o características novedosas que podrían ayudar a superar los objetivos de sostenibilidad. Para este fin, la biotecnología ...