[EN] Citrus tristeza virus (CTV), a phloem-restricted closterovirus infecting citrus, encodes three different silencing suppressors (p25, p20 and p23), one of which (p23) is a pathogenicity determinant that induces aberrations ...
CARBONELL, ALBERTO; La Peña Del Rivero, Marcos De; FLORES PEDAUYE, RICARDO; Gago, Selma(Oxford University Press, 2006-11)
[EN] Eggplant latent viroid (ELVd) can form stable hammerhead structures in its (+) and (¿) strands. These ribozymes have the longest helices I reported in natural hammerheads, with that of the ELVd (+) hammerhead being ...
[EN] Viroids, the smallest infectious agents endowed with autonomous replication, are tiny single-stranded circular RNAs (similar to 250 to 400 nt) without protein-coding ability that, despite their simplicity, infect and ...
The evolution of citrus tristeza virus (CTV) from outbreaks occurred in Calabria, Italy, was compared with that of CTV outbreaks reported previously in another two proximal Italian regions, Sicily and Apulia. Examination ...
Delgado Villar, Sonia Guadalupe; Navarro, Beatriz; Serra, Pedro; Gentit, Pascal; Cambra, Miguel Ángel; Chiumenti, Michela; De Stradis, Angelo; Di Serio, Francesco; FLORES PEDAUYE, RICARDO(Landes Bioscience, 2019-07-03)
[EN] Understanding how viruses and subviral agents initiate disease is central to plant pathology. Whether RNA silencing mediates the primary lesion triggered by viroids (small non-protein-coding RNAs), or just intermediate-late ...
[EN] Members of the family Avsunviroidae have a single-stranded circular RNA genome that adopts a rod-like or branched conformation and can form, in the strands of either polarity, hammerhead ribozymes involved in their ...
Di Serio, Francesco; Owens, Robert A.; Li, Shi-Fang; Matousek, Jaroslav; Pallás Benet, Vicente; Randles, John W.; Sano, Teruo; Verhoeven, Jacobus Th. J.; Vidalakis, Georgios; FLORES PEDAUYE, RICARDO(Microbiology Society, 2021)
[EN] Members of the family Pospiviroidae have single-stranded circular RNA genomes that adopt a rod-like or a quasi-rod-like conformation. These genomes contain a central conserved region that is involved in replication ...
Minoia, Sofia(Universitat Politècnica de València, 2011-09-06)
Los viroides son pequeños RNAs circulares que no codifican proteína alguna. Las infecciones causadas por viroides en plantas inducen la acumulación de pequeños RNAs viroidales (vd-sRNAs) de 21-24 nt que presentan características ...
[EN] Natural populations of peach latent mosaic viroid (PLMVd) are complex mixtures of variants. During routine testing, TaqMan rtRT-PCR and RNA gel-blot hybridization produced discordant results with some PLMVd isolates. ...
Nohales Zafra, Maria Angeles; Molina Serrano, Diego; Flores Pedauye, Ricardo; Daros Arnau, Jose Antonio(American Society for Microbiology, 2012)
Avocado sunblotch viroid, peach latent mosaic viroid, chrysanthemum chlorotic mottle viroid, and eggplant latent viroid (ELVd), the four recognized members of the family Avsunviroidae, replicate through the symmetric pathway ...
[EN] Conventional breeding of citrus types demands a long-term effort due to their complex reproductive biology and long juvenile period. As a compelling alternative, genetic engineering of mature tissues allows the insertion ...
[EN] Ten isolates of apple hammerhead viroid-like RNA (AHVd RNA) were detected by next-generation sequencing (NGS) and/or by RT-PCR analysis of apple (Malus domestica) 'Pacific Gala' (PG) from Canada. All consisted of 433 ...
[EN] Next-generation sequencing (NGS) has been applied to plant virology since 2009. NGS provides highly efficient, rapid, low cost DNA, or RNA high-throughput sequencing of the genomes of plant viruses and viroids and of ...
Flores Pedauye, Ricardo; Owens, Robert A.; Taylor, John(Elsevier, 2016-04)
The viroids of plants are the simplest known infectious genetic elements. They have RNA genomes of up to 400 nucleotides in length and no protein encoding capacity. Hepatitis delta virus (HDV), an infectious agent found ...
Carbonell Olivares, Alberto(Universitat Politècnica de València, 2008-05-07)
Los viroides, los agentes infecciosos conocidos más simples, están constituidos por una molécula circular de RNA monocatenario. A pesar de su pequeño tamaño (246-401 nt) y de no codificar proteínas, son capaces de replicarse ...
Eiras, Marcelo; Nohales Zafra, Maria Angeles; Kitajima, Elliot J.; Flores Pedauye, Ricardo; Daros Arnau, Jose Antonio(Springer Verlag (Germany), 2011-03)
[EN] Potato spindle tuber viroid (PSTVd) contains an element of tertiary structure -loop E- also present in eukaryotic 5S rRNA. The ribosomal protein L5 and transcription factor IIIA (TFIIIA) from Arabidopsis thaliana bind ...
[EN] Viroids and viroid-like satellite RNAs from plants, and the human hepatitis delta virus (HDV) RNA share some properties that include small size, circularity and replication through a rolling-circle mechanism. Replication ...
Navarro, B; Gisel, A; Rodio, Maria Elena; Delgado Villar, Sonia Guadalupe; Flores Pedauye, Ricardo; Di Serio, Francesco(Wiley, 2012-06)
[EN] How viroids, tiny non-protein-coding RNAs (250–400 nt), incite disease is unclear. One hypothesis is that
viroid-derived small RNAs (vd-sRNAs; 21–24 nt) resulting from the host defensive response, via RNA silencing,
may ...