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Browsing by Author "Asturiano Nemesio, Juan Francisco"

Now showing items 21-40 of 79

European eel sperm storage: optimization of short-term protocols and cryopreservation of large volumes

Herranz-Jusdado, Juan Germán; Gallego Albiach, Victor; Rozenfeld, Christoffer; MORINI, MARINA; Pérez Igualada, Luz María; Asturiano Nemesio, Juan Francisco (Elsevier, 2019)

[EN] Maturation in captivity of European eel (Anguilla anguilla) requires long and costly hormonal treatments that often lead to asynchronic maturation between sexes. Therefore, optimization of sperm short-term storage ...
Evaluation of methods to determine sperm density for the European eel, Anguilla anguilla

Sorensen, SR; Gallego Albiach, Victor; Pérez Igualada, Luz María; Butts, IAE; Tomkiewicz, J.; Asturiano Nemesio, Juan Francisco (Wiley, 2013-12)

[EN] Contents European eel, Anguilla anguilla, is a target species for future captive breeding, yet best methodology to estimate sperm density for application in in vitro fertilization is not established. Thus, our objectives ...
Exploring correlations between sex steroids and fatty acids and their potential roles in the induced maturation of the male European eel

Baeza Ariño, Rosa; Peñaranda, D.S.; Vilchez Olivencia, Maria Carmen; Tveiten, Helge; Pérez Igualada, Luz María; Asturiano Nemesio, Juan Francisco (Elsevier, 2015-01-01)

[EN] The present study was undertaken to evaluate the correlations between the fatty acids in the liver and testis and the plasma levels of the hormonal steroids used during eel spermatogenesis, in order to clarify the ...
First production of larvae using cryopreserved sperm. Effects of preservation temperature and cryopreservation on European eel sperm fertilization capacity

Asturiano Nemesio, Juan Francisco; Sorensen, S.R.; Pérez Igualada, Luz María; Lauesen, P.; Tomkiewicz, J. (Blackwell Publishing, 2016)

[EN] Contents Sperm cryopreservation is a useful tool in captive fish reproduction management, that is to synchronize gamete production, especially in the case of species as the European eel, where the time of female ...
Fish sperm motility assessment as a tool for aquaculture research, a historical approach

Gallego Albiach, Victor; Asturiano Nemesio, Juan Francisco (Blackwell Publishing, 2018)

[EN] Fish sperm motility is nowadays considered the best biomarker for the quality of fish spermatozoa, and sperm motion parameters from more than 300 fish species have been reported in more than 1500 scientific articles ...
Handling and treatment of male European eels (Anguilla anguilla) for hormonal maturation and sperm cryopreservation

Herranz-Jusdado, Juan Germán; Kása, É; Kollár, Timea; Gallego Albiach, Victor; Peñaranda, D.S.; Rozenfeld, Christoffer; Pérez Igualada, Luz María; Horváth, Ákos; Asturiano Nemesio, Juan Francisco (Journal of Visualized Experiments, 2018)

[EN] During the last years, several research groups have been working on the development and improvement of new protocols for the European eel handling and maturation. As of yet, weekly injections of human chorionic ...
Heterozygosity-fitness correlations in the gilthead sea bream Sparus aurata using microsatellite loci from unknown and gene-rich genomic locations

Borrell, Yaisel J.; Carleos, Carlos E.; Sanchez, José A.; Vázquez, Emilia; Gallego Albiach, Victor; Asturiano Nemesio, Juan Francisco; Blanco, Gloria (Wiley, 2011-11)

[EN] Heterozygosity-fitness correlations (HFC) were assessed for a sample of a gilthead sea bream Sparus aurata population. Two hundred and seventy-one fish were genotyped at 22 known and novel microsatellite loci, from ...
Hormonal manipulations for the enhancement of sperm production in cultured fish and evaluation of sperm quality

Mylonas, Constantinos C.; Duncan, Neil J.; Asturiano Nemesio, Juan Francisco (Elsevier, 2017)

[EN] This article reviews the use of hormonal treatments to enhance sperm production in aquaculture fish and the methods available for evaluating sperm quality. The different types of testis development are examined and a ...
Identification and stable expression of vitellogenin receptor (VTGR) through vitellogenesis in the European eel

MORINI, MARINA; Lafont, A. G.; Maugars, G.; Baloche, S.; Dufour, S.; Asturiano, Juan F.; Pérez Igualada, Luz María (Cambridge University Press, 2020-06)

[EN] In teleosts, vitellogenin (Vtg) is a phospholipoglycoprotein synthesized by the liver, released into the blood circulation and incorporated into the oocytes via endocytosis mediated by the Vtg receptor (VTGR) to form ...
Identification of the major proteins present in the seminal plasma of European eel, and how hormonal treatment affects their evolution. Correlation with sperm quality

Vilchez Olivencia, Maria Carmen; D. Pla; Gallego Albiach, Victor; Sanz, Libia; Pérez Igualada, Luz María; Asturiano Nemesio, Juan Francisco; Calvete, Juan J.; Peñaranda, D.S. (Elsevier, 2016-11)

[EN] By first time, 2DE protein profile of European eel seminal plasma has been determined. 14 different proteins corresponding to 9 major families were identified in seminal plasma, through hormonal treatment. Some of ...
Impact of dietary fatty acids on muscle composition, liver lipids, milt composition and sperm performance in European eel

Butts, Ian Anthony Ernest; Baeza Ariño, Rosa; Stottrup, Josianne Gatt; Kruger-Johnsen, Maria; Jacobsen, Charlotte; Pérez Igualada, Luz María; Asturiano Nemesio, Juan Francisco; Tomkiewicz, Jonna (Elsevier, 2015-05)

[EN] In order for European eel aquaculture to be sustainable, the life cycle should be completed in captivity. Development of broodstock diets may improve the species' reproductive success in captivity, through the production ...
Improvement of techniques for sperm evaluation and cryobanking in European eel

Herranz Jusdado, Juan Germán (Universitat Politècnica de València, 2019-11-12)

[ES] En las últimas décadas, la anguila europea Anguilla anguila ha sufrido una disminución drástica de su población lo que ha llevado su inclusión como especie en peligro crítico en la lista roja UICN. Esta situación, ...
Inducción hormonal de la espermiación y criopreservación de esperma en anguila europea (Anguilla anguilla)

Garzón Cortés, Victor Daniel Ladislao (Universitat Politècnica de València, 2008-05-07)

La producción de anguilas se basa en la captura de angulas, pero la captura de éstas no se ha podido incrementar al mismo ritmo que su demanda para el engorde en piscifactorías. La caída de las capturas de angulas llevó a ...
Influence of temperature regime on endocrine parameters and vitellogenesis during experimental maturation of European eel (Anguilla anguilla) females

Pérez Igualada, Luz María; Peñaranda, D.S.; Dufour, S.; Baloche, S.; Palstra, A. P.; Van den Thillart, G.E.E.J.M.; Asturiano Nemesio, Juan Francisco (Elsevier, 2011-10-01)

[EN] We examined the effect of temperature in European silver eels during their maturation induced by injections of carp pituitary extract on endocrine parameters: pituitary fshß and lhß expression, plasma 17ß-estradiol ...
Influence of the ionic and protein environment on sperm motility activation in the European eel

Vílchez Olivencia, María del Carmen (Universitat Politècnica de València, 2017-11-03)

In general, fish spermatozoa are immotile in the testis. Movement is activated due to the osmotic shock (hypo- or hyperosmotic, depending on fish origin: freshwater or sea water species) experienced when they are released ...
Informe de prácticas empresarial realizadas en el Instituto de investigación de tecnologia agroalimentaria IRTA

SEBASTIAO, HELDER JOSEFO DAVID (Universitat Politècnica de València, 2012-05-17)

Se llevaron a cabo actividades relacionadas con la producción de fitoplancton, producción de artemia y rotíferos, y se participó en prácticas relacionadas con aspectos sanitarios, manejo de sistemas de recirculación acuícola, ...
Intracellular changes in Ca2+, K+ and pH after sperm motility activation in the European eel (Anguilla anguilla): Preliminary results

Gallego Albiach, Victor; Martínez Pastor, Felipe; Mazzeo, Ilaria; Peñaranda, D.S.; Herraez, Paz; Asturiano Nemesio, Juan Francisco; Pérez Igualada, Luz María (Elsevier, 2014-01-01)

[EN] Although it is widely accepted that osmolality and ion fluxes are the main factors triggering sperm motility in fish, a complex universal mechanism for sperm motility activation does not exist in fish, and studies of ...
Intracellular pH regulation and sperm motility in the European eel

Pérez Igualada, Luz María; Gallego Albiach, Victor; Asturiano, Juan F. (Elsevier, 2020-03-15)

[EN] Sperm activation involves ion fluxes as well as a previous maturation in the seminal plasma, something which has not been studied in depth in marine fish species. pH and potassium are probably involved in sperm ...
Japanese eel (Anguilla japonica Temminck & Schlegel, 1846) propagation by using cryopreserved sperm samples

Müller, T.; Matsubara, H.; Kubara, Y.; Horváth, Ákos; Asturiano Nemesio, Juan Francisco; Urbányi, B. (Blackwell Publishing, 2017)

[EN] The aim of this study was to test the artificial seminal plasma (ASP) as the extender as well as methanol as the cryoprotectant for cryopreservation, and to collect information on the fertilizing capacity of cryopreserved ...
Larval width as indicator of growth rate and effect of larval classification on final body composition and flesh quality in cultured gilthead seabream (Sparus aurata, L.)

Mazzeo, Ilaria; Borrell, Yaisel J.; Gallego Albiach, Victor; Garcia Fernandez, Carlos; Sánchez, José Antonio; Blanco, Gloria; Pérez Igualada, Luz María; Asturiano Nemesio, Juan Francisco (Wiley, 2014-04)

[EN] The objective of this study was evaluating the effects on the growth rate and flesh quality of separating larvae according to their larval width in cultured gilthead seabream (Sparus aurata, L.). Progeny from two ...