González Martínez, Miguel Ángel; Morais, Sergi; Puchades, Rosa; Maquieira Giménez, Manuel; Abad Fuentes, Antonio; Montoya Baides, Ángel(Elsevier Masson, 1997-07-30)
[EN] The application of controlled-pore glass (CPG) as solid support for immobilization of immunoreagents in order to develop flow-through immunosensors is described. Monoclonal antibodies (MAbs) to carbaryl were site-directed ...
Guillen Guille, Isabel; Martínez SanMartín, Ángel; Gabaldón Hernández, José Antonio; Núñez Delicado, Estrella; Puchades Pla, Rosa; Maquieira Catala, Ángel(Ştefan cel Mare University – Suceava, 2011)
[EN] A lateral flow immunoassay (LFIA) was developed in the competitive reaction format and applied to test residues of the antimicrobial oxyteracycline (OTC) in honey samples. To prepare the assay test, a nitrocellulose ...
Selective covalent immobilization of thiolated oligonucleotides onto an epoxy-functionalized silicon-substrate can be achieved via light radiation (365 nm). Following this approach, thiol-modified oligonucleotide probes ...
[EN] A novel label-free biosensing approach based on bioreceptor networks patterned as diffractive gratings (biogratings) has been developed. Nanogrooved structures were used as optically active scaffolds for producing ...
DNA binding chemistry on silicon surface has been investigated. Aminated oligonucleotide probes were immobilized on the chip surface by chemical silanization and further covalent attachment. The chemistries employed were ...
[EN] A rapid strategy for the covalent immobilization of DNA onto silicon-based materials using the UV-initiated radical thiol-ene reaction is presented in this study. Following this approach, thiol- and alkene-modified ...
Direct hapten-linked multiplexed immunoassay is developed on the polycarbonate surface of standard Digital Versatile Discs (DVDs) for six compounds of environmental concern, as proof of concept. Carboxylated haptens are ...
[EN] The idea of using disk drives to monitor molecular biorecognition events on regular optical discs has received considerable attention during the last decade. CDs, DVDs, Blu-ray discs and other new optical discs are ...
Dispersive solid-phase extraction (dSPE) using fatty acid-coated Eu2O3 nanoparticles (NPs) was developed, and a direct immunoassay was carried out employing these NPs as support. Secondary antibodies labeled with fluorophore ...
Jornet Olivé, Maria Dolors(Editorial Universitat Politècnica de València, 2010-07-30)
La determinación y control de contaminantes orgánicos ha despertado gran interés en los últimos años. Estos compuestos se acumulan en los ecosistemas terrestres y acuáticos, cosa que los hace especialmente perjudiciales ...
Publicació divulgativa de perfils de científiques en diferents disciplines vinculades amb les àrees de coneixement pròpies de la Universitat Politècnica de València.
Escorihuela Fuentes, Jorge; González Martínez, Miguel Ángel; López-Paz, José Luis; Puchades, Rosa; Maquieira Catala, Ángel; Giménez Romero, David(American Chemical Society, 2015-01-14)
[EN] The challenging lecture given in 1959 by physicist and Nobel Prize awarded R. P. Feynman:
“There's plenty of room at the bottom” is considered to be the starting point for
nanotechnology. With this peculiar title, ...
[EN] The quartz crystal microbalance (QCM) technique has been applied for monitoring the biorecognition of ArtinM lectins at low horseradish peroxidase glycoprotein (HRP) concentrations, using a simple kinetic model based ...
Endocrine disruptors (EDs) interfere with the development and functioning of the endocrine system, causing reproductive disturbance in aquatic wildlife. The aim of the present work was to determine the presence of EDs in ...
[EN] An approach to enhance the sensitivity of optical biosensors is developed. It is based on patterning strips of
biochemical probes and signal processing (frequency-domain analysis, FDA) to the resulting analytical ...
González Pedro, María Victoria(Universitat Politècnica de València, 2012-07-26)
El desarrollo de nuevos materiales para la mejora de sistemas analíticos basados en receptores moleculares -principalmente proteínas y ácidos nucleicos- altamente sensibles, con gran capacidad de trabajo y de manejo sencillo, ...
[EN] Spatially controlled anchoring of NA probes onto microscope glass slides by a novel fluor-thiol coupling reaction is performed. By this UV-initiated reaction, covalent immobilization in very short times (30 s at 254 ...
Cháfer Pericás, María Consuelo; Maquieira Catala, Ángel; Puchades, Rosa(Elsevier, 2012-01)
[EN] In recent years, research has focused on the development of different types of inorganic nanoparticles (NPs) to be used as labels in immunoassays instead of fluorophores or organic particles. Basically, inorganic NPs ...
Brun Sánchez, Eva María; Puchades Pla, Rosa; Maquieira Catala, Ángel(American Chemical Society, 2013-03-15)
Compact disc (CD) surface modification with
different reflective materials offers new working perspectives as
bioanalytical platforms. The potential of gold, carbon, and
aluminum low-reflectivity compact discs as a ...