Zwart, Mark Peter; Willemsen, Anouk; Daros Arnau, Jose Antonio; Elena Fito, Santiago Fco(Oxford University Press (OUP): Molecular Biology and Evolution, 2014-01)
[EN] Viruses have evolved highly streamlined genomes and a variety of mechanisms to compress them, suggesting that genome size is under strong selection. Horizontal gene transfer has, on the other hand, played an important ...
Rodrigo Tarrega, Guillermo; Majer, Eszter; Prakash, Satya; Daros Arnau, Jose Antonio; Jaramillo, Alfonso; Poyatos, Juan F.(Biophysical Society, 2015-09-01)
[EN] The regulation of gene expression, triggered by conformational changes in RNA molecules, is widely observed in cellular systems. Here, we examine this mode of control by means of a model-based design and construction ...
Ortega Miralles, Iván(Universitat Politècnica de València, 2023-02-08)
[ES] El silenciamiento génico o RNA de interferencia (RNAi) ha sido descrito como un medio para suprimir la actividad de un gen tanto en plantas como en animales, y está basado en pequeños RNAs (sRNAs) de 20-35 nucleótidos ...
Gayubas Balaguer, Beatriz(Universitat Politècnica de València, 2017-09-05)
[ES] La edición dirigida de genomas en plantas va a revolucionar en los próximos años el campo de
la mejora de las variedades vegetales y la biotecnología vegetal. De entre las diferentes técnicas
de edición de genomas, ...
[EN] A gene construct encoding a xylanase, which is active in extreme conditions of temperature and alkaline pH (90 °C, pH 10.5), has been transitorily expressed with high efficiency in Nicotiana benthamiana using a viral ...
Beceiro Durán, Cristina(Universitat Politècnica de València, 2017-02-28)
[EN] Plant viruses can be manipulate
d and converted into biotechnological tools to
produce recombinant proteins in biofactory plan
t
s. One of the viruses most frequently
used is
Tobacco mosaic ...
CARBONELL, ALBERTO; Carrington, James C.; Daros Arnau, Jose Antonio(Smart Science and Technology, 2016-01-12)
[EN] Artificial small RNAs (sRNAs) are short ≈21-nt non-coding RNAs engineered to inactivate sequence
complementary RNAs. In plants, they have been extensively used to silence cellular transcripts in gene function
analyses ...
Carbonell, Alberto; López Del Rincón, Carmelo; Daròs, José-Antonio(Scientific Societies, 2019-02)
[EN] Artificial small RNAs (sRNAs), including artificial microRNAs (amiRNAs) and
synthetic trans-acting small interfering RNAs (syn-tasiRNAs), are used to
silence viral RNAs and confer antiviral resistance in plants. ...
Martinez Garcia, Fernando; Elena Fito, Santiago Fco; Daros Arnau, Jose Antonio(American Phytopathological Society, 2013)
Artificial microRNAs (amiRNAs) are the expression products of engineered microRNA (miRNA) genes that efficiently and specifically down-regulate RNAs that contain complementary sequences. Transgenic plants expressing high ...
Pérez De Castro, Ana María; Esteras Gómez, Cristina; Alfaro Fernández, Ana Olvido; Daròs, José-Antonio; Monforte Gilabert, Antonio José; Picó Sirvent, María Belén; Gómez-Guillamón, María Luisa(Springer-Verlag, 2019-07)
[EN] Recessive resistance to Watermelon mosaic virus (WMV) in melon has previously been reported in the African accession TGR-1551. Using a population of recombinant inbred lines (RIL), derived from a cross between TGR-1551 ...
[EN] Eukaryotic RNA interference (RNAi) results in gene silencing upon the sequence-specific degradation of target transcripts by complementary small RNAs (sRNAs). In plants, RNAi-based tools have been optimized for high ...
Rodrigo Tarrega, Guillermo; Landrain, Thomas E.; Majer, Eszter; Daros Arnau, Jose Antonio; Jaramillo, Alfonso(Public Library of Science, 2013-08)
[EN] Small RNAs (sRNAs) can operate as regulatory agents to control protein expression by interaction with the 59 untranslated
region of the mRNA. We have developed a physicochemical framework, relying on base pair ...
[EN] Recent metagenomic studies have provided an unprecedented wealth of data, which are revolutionizing our understanding of virus diversity. A redrawn landscape highlights viruses as active players in the phytobiome, and ...
Ortolá Navarro, Beltrán(Universitat Politècnica de València, 2023-03-27)
[ES] Los viroides, genomas mínimos de RNA circular no codificante, monocatenarios y muy estructurados, parasitan factores celulares de las plantas para replicarse autónomamente, establecer infecciones sistémicas y usualmente ...
Cervera-Seco, Luis; Marques, M.C.; Sanz-Carbonell, Alejandro; Márquez-Molins, Joan; CARBONELL, ALBERTO; DAROS ARNAU, JOSE ANTONIO; Gomez, Gustavo Germán(Oxford University Press, 2019-11)
[EN] Small interfering RNAs (siRNA) are key regulators of gene expression that play essential roles in diverse biological processes. Trans-acting siRNAs (tasiRNAs) are a class of plant-endogenous siRNAs that lead the ...
Lafforgue, Guillaume; Martínez, Fernando; Niu, Qi Wen; Chua, Nam Hai; Daros Arnau, Jose Antonio; Elena Fito, Santiago Fco(American Society for Microbiology, 2013-07)
[EN] A drawback of recent antiviral therapies based on the transgenic expression of artificial microRNAs (amiRs) is the ease with
which viruses generate escape mutations. Here, we show two alternative strategies for ...
Pallás Benet, Vicente; HERNANDEZ FORT, CARMEN; Marcos, Jose F.; Daròs, José-Antonio; Ambrós, Silvia; Navarro, Beatriz; NAVARRO BOHIGUES, JOSE ANTONIO; La Peña Del Rivero, Marcos De; Gago-Zachert, Selma; Gas, Maria E.; CARBONELL, ALBERTO; López Del Rincón, Carmelo; Martínez-De Alba, Angel E.; Di Serio, Francesco; Moreno, Pedro(Elsevier, 2022-04-15)
[EN] Ricardo Flores (1947-2020) focused his research on the identification, replication, pathogenesis, and evolution of viroids, the minimal non-protein-coding circular RNAs (250-400 nt) able to replicate and incite diseases ...
[EN] RNA interference (RNAi) is a natural mechanism for protecting against harmful genetic elements and regulating gene expression, which can be artificially triggered by the delivery of homologous double-stranded RNA ...
Nohales Zafra, Maria Angeles; Molina Serrano, Diego; Flores Pedauye, Ricardo; Daros Arnau, Jose Antonio(American Society for Microbiology, 2012)
Avocado sunblotch viroid, peach latent mosaic viroid, chrysanthemum chlorotic mottle viroid, and eggplant latent viroid (ELVd), the four recognized members of the family Avsunviroidae, replicate through the symmetric pathway ...