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Browsing by UPV Unit "Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes"

Now showing items 41-60 of 994

A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard

Vázquez-Vilar, Marta; Bernabé-Orts, Joan Miquel; Fernández Del Carmen, María Asunción; Ziarsolo Areitioaurtena, Pello; Blanca Postigo, José Miguel; Granell Richart, Antonio; Orzáez Calatayud, Diego Vicente (Springer (Biomed Central Ltd.), 2016)

[EN] Background: The efficiency, versatility and multiplexing capacity of RNA-guided genome engineering using the CRISPR/Cas9 technology enables a variety of applications in plants, ranging from gene editing to the ...
A Multifunctionalized Potyvirus-Derived Nanoparticle That Targets and Internalizes into Cancer Cells

Truchado, Daniel A.; Juarez-Molina, Maria; Rincón, Sara; Zurita, Lucia; Tomé-Amat, Jaime; Lorz, Corina; Ponz, Fernando (MDPI, 2024-04)

[EN] Plant viral nanoparticles (VNPs) are attractive to nanomedicine researchers because of their safety, ease of production, resistance, and straightforward functionalization. In this paper, we developed and successfully ...
A new genomic library of melon introgression lines in a cantaloupe genetic background for dissecting desirable agronomical traits

Perpiñá Martín, Gorka; Esteras Gómez, Cristina; Gibon, Y.; Monforte Gilabert, Antonio José; Picó Sirvent, María Belén (Springer (Biomed Central Ltd.), 2016)

[EN] Background: Genomic libraries of introgression lines (ILs) consist of collections of homozygous lines with a single chromosomal introgression from a donor genotype in a common, usually elite, genetic background, ...
A new role for green leaf volatile esters in tomato stomatal defense against Pseudomonas syringe pv. tomato

López-Gresa, María Pilar; Payá, C.; Ozáez-Martínez, Miguel; Rodrigo Bravo, Ismael; Conejero Tomás, Vicente; Klee, H; Belles Albert, José Mª; Lisón, Purificación (Frontiers Media SA, 2018)

[EN] The volatile esters of (Z)-3-hexenol with acetic, propionic, isobutyric, or butyric acids are synthesized by alcohol acyltransferases (AAT) in plants. These compounds are differentially emitted when tomato plants are ...
A non-coding RNA induces changes in dynamic DNA methylation of ribosomal RNA genes in host plants

Martínez Arias, Germán Eugenio; Castellano Pérez, Mayte; Tortosa-Viqueira, Maria; Pallás Benet, Vicente; Gomez, Gustavo Germán (Oxford University Press (OUP), 2014-02)

[EN] Viroids are plant-pathogenic non-coding RNAs able to interfere with as yet poorly known host-regulatory pathways and to cause alterations recognized as diseases. The way in which these RNAs coerce the host to express ...
A Non-Targeted Approach Unravels the Volatile Network in Peach Fruit

Sánchez, Gerardo; Besada Ferreiro, Cristina María; Badenes, María Luisa; Monforte Gilabert, Antonio José; Granell Richart, Antonio (Public Library of Science, 2012-06)

[EN] Volatile compounds represent an important part of the plant metabolome and are of particular agronomic and biological interest due to their contribution to fruit aroma and flavor and therefore to fruit quality. By ...
A Non-targeted Metabolomics Approach Unravels the VOCs Associated with the Tomato Immune Response against Pseudomonas syringae

López-Gresa, María Pilar; Lisón, Purificación; Campos Beneyto, Laura; Rodrigo Bravo, Ismael; Rambla Nebot, Jose Luis; Granell Richart, Antonio; Conejero Tomás, Vicente; Belles Albert, José Mª (Frontiers Media SA, 2017)

[EN] Volatile organic compounds (VOCs) emitted by plants are secondary metabolites that mediate the plant interaction with pathogens and herbivores. These compounds may perform direct defensive functions, i. e., acting as ...
A noncoding plant pathogen provokes both transcriptional and posttranscriptional alterations in tomato

Lisón Párraga, María Purificación; Tarraga Herrero, Susana; López Gresa, Mª Pilar; Sauri Ferrando, Asunción; Torres Vidal, Cristina; Campos Beneyto, Laura; Belles Albert, José Mª; Conejero Tomás, Vicente; Rodrigo Bravo, Ismael (Wiley-Blackwell, 2013-03)

Viroids are single-stranded, circular, noncoding RNAs that infect plants, causing devastating diseases. In this work, we employed 2D DIGE, followed by MS identification, to analyze the response of tomato plants infected ...
A novel role for protein kinase Gcn2 in yeast tolerance to intracellular acid stress

Hueso Lorente, Guillem; Aparicio Sanchis, Rafael; Montesinos De Lago, Consuelo; Lorenz, Silvia; Murguía Ibáñez, José Ramón; Serrano Salom, Ramón (Portland Press, 2012)

Intracellular pH conditions many cellular systems, but its mechanisms of regulation and perception are mostly unknown. We have identified two yeast genes important for tolerance to intracellular acidification caused by ...
A One Precursor One siRNA Model for Pol IV-Dependent siRNA Biogenesis.

Zhai, Jixian; Bischof, Sylvain; Wang, Haifeng; Feng, Suhua; Lee, Tzuu-Fen; Teng, Chong; Chen, Xinyuan; Park, Soo Young; Liu, Linshan; Gallego Bartolomé, Javier; Liu, Wanlu; Henderson, Ian R; Meyers, Blake C.; Ausin, Israel; Jacobsen, Steven E. (Elsevier, 2015-10-08)

[EN] RNA-directed DNA methylation in Arabidopsis thaliana is driven by the plant-specific RNA Polymerase IV (Pol IV). It has been assumed that a Pol IV transcript can give rise to multiple 24-nt small interfering RNAs ...
A pathogenic long non-coding RNA redesigns the epigenetic landscape of the infected cells by subverting host Histone Deacetylase 6 activity

Castellano Pérez, Mayte; Pallás Benet, Vicente; Gomez, Gustavo Germán (Wiley, 2016-09)

[EN] Viroids - ancient plant-pathogenic long noncoding RNAs - have developed a singular evolutionary strategy based on reprogramming specific phases of host-metabolism to ensure that their infection cycle can be completed ...
A permeable cuticle is associated with the release of reactive oxygen species and induction of innate immunity

L'Haridon, Floriane; Besson-Bard, Angelique; Binda, Matteo; Serrano Mateo, Mario; Abou-Mansour, Eliane; Balet, Francine; Schoonbeek, Henk-Jan; Hess, Stephane; Mir Moreno, Ricardo; Leon Ramos, Jose; Lamotte, Olivier; Metraux, Jean-Pierre (Public Library of Science, 2011)

[EN] Wounded leaves of Arabidopsis thaliana show transient immunity to Botrytis cinerea, the causal agent of grey mould. Using a fluorescent probe, histological staining and a luminol assay, we now show that reactive oxygen ...
A pospiviroid from symptomless portulaca plants closely related to iresine viroid 1

Verhoeven, J.TH J.; Roenhorst, JW; Hooftman, M; Meekes, E.T.M.; Flores Pedauye, Ricardo; Serra Alfonso, Pedro (Elsevier, 2015-07-02)

[EN] In symptomless plants of portulaca a potential new pospiviroid was characterized. Analysis by both double and return PAGE showed the presence of a circular RNA. RT-PCR and sequencing revealed a genome of 351 nt with ...
A potyvirus vector efficiently targets recombinant proteins to chloroplasts, mitochondria and nuclei in plant cells when expressed at the amino terminus of the polyprotein

Majer, Eszter; Navarro Bohigues, José Antonio; Daros Arnau, Jose Antonio (Wiley-VCH Verlag, 2015-11)

Plant virus-based expression systems allow quick and efficient production of recombinant proteins in plant biofactories. Among them, a system derived from tobacco etch virus (TEV; genus potyvirus) permits coexpression of ...
A putative antiviral role of plant cytidine deaminases

Martín, Susana; Cuevas, J.M.; Grande-Perez, A.; Elena Fito, Santiago Fco (F1000 Research, Ltd., 2017)

[EN] Background: A mechanism of innate antiviral immunity operating against viruses infecting mammalian cells has been described during the last decade. Host cytidine deaminases (e.g., APOBEC3 proteins) edit viral genomes, ...
A Recombinant Potato virus Y Infectious Clone Tagged with the Rosea1 Visual Marker (PVY-Ros1) Facilitates the Analysis of Viral Infectivity and Allows the Production of Large Amounts of Anthocyanins in Plants

Cordero, Teresa; Mohamed, M.A.; Lopez Moya, J.J.; DAROS ARNAU, JOSE ANTONIO (Frontiers Media SA, 2017-04-06)

[EN] Potato virus Y (PVY) is a major threat to the cultivation of potato and other solanaceous plants. By inserting a cDNA coding for the Antirrhinum majus Rosea1 transcription factor into a PVY infectious clone, we created ...
A remarkable synergistic effect at the transcriptomic level in peach fruits doubly infected by Prunus necrotic ringspot virus and Peach latent mosaic viroid

Herranz Gordo, Maria Del Carmen; Niehl, A; Rosales, Marlene; Fiore, Nicola; Zamorano, A; Granell Richart, Antonio; Pallás Benet, Vicente (BioMed Central, 2013-05-28)

[EN] Background: Microarray profiling is a powerful technique to investigate expression changes of large amounts of genes in response to specific environmental conditions. The majority of the studies investigating gene ...
A sensitive and rapid RNA silencing suppressor activity assay based on alfalfa mosaic virus expression vector

Martínez-Pérez, Mireya; NAVARRO BOHIGUES, JOSE ANTONIO; Pallás Benet, Vicente; SANCHEZ NAVARRO, JESUS ANGEL (Elsevier, 2019-10-15)

[EN] Plant viruses express RNA silencing suppressor (RSS) proteins to counteract plant defence mechanisms. Here, we describe a method to assess the RSS activity based on an alfalfa mosaic virus (AMV) RNA 3 expression vector ...
A synthetic biology approach for consistent production of plant-made recombinant polyclonal antibodies against snake venom toxins

Julve Parreño, Jose Manuel; Huet, Estefania; Fernandez-del-Carmen, Asun; Segura, Alvaro; Venturi, Micol; Gandia, Antoni; Pan, Wei-song; Albaladejo, Irene; Forment Millet, José Javier; Pla, Davinia; Wigdorovitz, Andrés; Calvete, Juan J.; Gutierrez, Carlos; Gutiérrez, José María; GRANELL RICHART, ANTONIO; Orzáez Calatayud, Diego Vicente (Blackwell Publishing, 2018-03)

[EN] Antivenoms developed from the plasma of hyperimmunized animals are the only effective treatment available against snakebite envenomation but shortage of supply contributes to the high morbidity and mortality toll of ...
A systems biology approach to the evolution of plant-virus interactions

Elena Fito, Santiago Fco; CARRERA, J.; Rodrigo, J (Elsevier, 2011-08)

[EN] Omic approaches to the analysis of plant-virus interactions are becoming increasingly popular. These types of data, in combination with models of interaction networks, will aid in revealing not only host components ...