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Browsing by Subject "Cryopreservation"

Now showing items 1-18 of 18

Aplicación de Corifolitropina alfa en la producción de embriones de conejo (Oryctolagus cuniculus).

Fortea González, Lydia (2017-04-12)

[ES] En los últimos años, los tratamientos de superovulación, inseminación artificial, producción de embriones y su criconservación, han constituido las herramientas biotecnológicas más utilizadas tanto en reproducción ...
Calcium concentration in vitrification medium affects the developmental competence of in vitro matured ovine oocytes

Succu, Sara; Berlinguer, Fiammetta; Leoni, Giovanni G.; Bebbere, Daniela; Satta, Valentina; Marco Jiménez, Francisco; Pasciu, Valeria; Naitana, Salvatore (Elsevier, 2011-03-01)

[EN] The present study was designed to determine whether different calcium concentrations in the vitrification solutions could improve the developmental competence of in vitro matured ovine oocytes after cryopreservation. ...
Comparison of two techniques for the morphometry study on gilthead seabream (Sparus aurata) spermatozoa and evaluation of changes induced by cryopreservation.

Gallego Albiach, Victor; Peñaranda, D.S.; Marco Jiménez, Francisco; Mazzeo, Ilaria; Pérez Igualada, Luz María; Asturiano Nemesio, Juan Francisco (Elsevier, 2012-04-01)

[EN] The development of powerful software has made possible spermatozoa morphology studies. However, some problems have emerged in relation to protocol standardization to compare results from different laboratories. This ...
Does storage time in LN2 influence survival and pregnancy outcome of vitrified rabbit embryos?

Lavara García, Raquel; Baselga Izquierdo, Manuel; Vicente Antón, José Salvador (Elsevier, 2011-09)

Vitrification is one of the most widely used techniques for embryo cryopreservation. The aim of this work was to study the effect of storage time in liquid nitrogen on vitrified rabbit embryos. A total of 1467 vitrified ...
Efecto de la crioconservación sobre el proteoma de hígado en conejo (Oryctolagus cuniculus).

Borrás Pérez, Sara (2017-09-06)

[ES] Conservar y mantener gametos y embriones, así como tejido reproductivo, es una herramienta cada vez más utilizada en el área de la reproducción asistida, siendo concretamente la embrionaria en fases tempranas la ...
Efecto transgeneracional de la vitrificación sobre el proteoma de hígado en el modelo conejo (Oryctolagus cuniculus)

Muniesa Andreu, Marc (2018-09-17)

[ES] En la actualidad, las elevadas tasas de infertilidad han propiciado un aumento del uso de las técnicas de reproducción asistida (ARTs), con más de 6,5 millones de nacimientos contabilizados el año 2013. Una herramienta ...
Effect of cholesterol-loaded cyclodextrins on buck sperm quality after cryopreservation with different extenders

Konyali, Coskun (2012-01-18)

This study was contucted to determine the effects of cholesterol-loaded cyclodextrins (CLC) on quality of buck sperm frozen with different extenders. Buck sperm were treated prior to cryopreservation with CLC and frozen ...
Effect of cytochalasin b pre-treatment of in vitro matured porcine oocytes before vitrification

Marco Jiménez, Francisco; CASARES CRESPO, L.; Vicente Antón, José Salvador (Cryo Letters, 2012-01)

[EN] The study tested the efficacy of pre-treating mature porcine oocytes with cytochalasin B before vitrification by the open pulled straw method (OPS) in a low toxicity solution containing ice blockers. The effects of ...
Effect of the primary cooling rate on the motility and fertility of frozen-thawed rabbit spermatozoa

Maeda, Tatsuhiro; Liu, Enqi; Nishijima, Kazutoshi; Tanaka, M.; Yamaguchi, Shinji; Morimoto, Masatoshi; Watanabe, Teruo; Fan, Jianglin; Kitajima, Shuji (Editorial Universitat Politècnica de València, 2012-06-30)

[EN] In the present study, we examined the effect of primary cooling rates on the motility and fertility of frozen-thawed rabbit spermatozoa. Rabbit semen diluted with an egg-yolk acetamide extender was cooled from room ...
EMBRYOLOGICAL AND MICROMANIPULATION TECHNIQUES IN ZEBRAFISH (Danio rerio) AND PACIFIC OYSTER (Crassostrea gigas)

Cardona Costa, José (2010-05-24)

En este trabajo de tesis, se presentan diversos estudios experimentales, desarrollados prin cipalmente en pez cebra pero también en ostra del Pacífico, que persiguen la puesta a punto de técnicas relevantes para la utilización ...
Estudio del efecto de la crioconservación sobre la expresión génica de embriones de conejo

Saenz de Juano Ribes, María de los Desamparados (2014-04-10)

La crioconservación de los embriones por congelación o vitrificación reduce la supervivencia de los embriones de conejo (Oryctolagus cuniculus) entre un 20 y 50% dependiendo de la estirpe genética y el procedimiento ...
First production of larvae using cryopreserved sperm. Effects of preservation temperature and cryopreservation on European eel sperm fertilization capacity

Asturiano Nemesio, Juan Francisco; Sorensen, S.R.; Pérez Igualada, Luz María; Lauesen, P.; Tomkiewicz, J. (Blackwell Publishing, 2016)

[EN] Contents Sperm cryopreservation is a useful tool in captive fish reproduction management, that is to synchronize gamete production, especially in the case of species as the European eel, where the time of female ...
Generation of offspring from cryopreserved rabbit (Oryctolagus cuniculus) oocytes

Jiménez Trigos, María Estrella (2014-06-09)

The general aim of this thesis was to optimise the current methodologies of oocyte cryopreservation in order to obtain live offspring from cryopreserved rabbit oocytes. In chapter 1, meiotic spindle configuration, ...
Long-term and transgenerational effects of cryopreservation on rabbit embryos

Lavara García, Raquel; Baselga Izquierdo, Manuel; Marco Jiménez, Francisco; Vicente Antón, José Salvador (Elsevier, 2014-04-15)

The short-term effects of cryopreservation and embryo transfer are well documented (reduced embryo viability, changes in pattern expression), but little is known about their long-term effects. We examined the possibility ...
Post-warming competence of in vivo matured rabbit oocytes treated with cytoskeletal stabilisation (Taxol) and cytoskeletal relaxant (Cytochalasin B) before vitrification

Jiménez Trigos, María Estrella; Naturil Alfonso, Carmen; Vicente Antón, José Salvador; Marco Jiménez, Francisco (Wiley, 2013-02)

[EN] Contents The aim of this study was to investigate the effect of Taxol and Cytochalasin B on the spindle, chromosome configuration and development to blastocyst stage after parthenogenesis activation of in vivo matured ...
Quality assessment of cryopreserved New Zealand white rabbit spermatozoa in INRA-82 extender containing different cryoprotectants

Fadl, Aya Mohamed; Ghallab, Abdel-Raouf Morsy; Abou-Ahmed, Mostafa Mohamed (Universitat Politècnica de València, 2019-06-28)

[EN] The present study aimed to evaluate the effect of supplementation of INRA-82 semen extender with different cryoprotectants (dimethyl sulphoxide; DMSO vs. dimethyl formamide; DMF) on the quality of white New Zealand ...
Study of male reproductive physiology and sperm conservation in the european EEL ( Anguilla anguilla, L.)

Sánchez Peñaranda, David (2008-09-12)

La anguila europea es una especie de gran importancia comercial en la acuicultura europea, pero el dramático descenso de las poblaciones naturales ha provocado la inclusión de esta especie en la lista CITES (convención del ...
Treating boar sperm with cholesterol-loaded cyclodextrins or cyclodextrins prior to cryopreservation: effects on post-thaw in vitro sperm quality of sperm cryopreserved in different freezing extenders.

Blanch Torres, Eva (2015-12-14)

[EN] Cryopreserved boar sperm is not used extensively for artificial insemination due to poor fertility rates of the sperm after freezing and thawing. The sperm membrane is damaged when cooled from body temperature to 5 ...