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Browsing by Subject "Gene expression"

Now showing items 1-20 of 57

A Cost-effective Double-Stranded cDNA Synthesis for Plant Microarrays

Gonzalez-Ibeas, Daniel; Blanca Postigo, José Miguel; Cañizares Sales, Joaquín; Truniger, Veronica; Aranda, Miguel A. (Springer Verlag (Germany), 2012-10)

[EN] DNA microarrays are two-dimensional arrangements of specific probes deposited on a substrate that have been widely used in gene expression analysis by measuring mRNA accumulation. The use of this type of microarrays ...
A meta-analysis reveals the commonalities and differences in Arabidopsis thaliana response to different viral pathogens

Rodrigo Tarrega, Guillermo; Carrera Montesinos, Javier; Ruiz-Ferrer, Virginia; Del Toro, F.J.; Llave, C; Voinnet, O.; Elena Fito, Santiago Fco (Public Library of Science, 2012-07)

Understanding the mechanisms by which plants trigger host defenses in response to viruses has been a challenging problem owing to the multiplicity of factors and complexity of interactions involved. The advent of genomic ...
A permeable cuticle is associated with the release of reactive oxygen species and induction of innate immunity

L'Haridon, Floriane; Besson-Bard, Angelique; Binda, Matteo; Serrano Mateo, Mario; Abou-Mansour, Eliane; Balet, Francine; Schoonbeek, Henk-Jan; Hess, Stephane; Mir Moreno, Ricardo; Leon Ramos, Jose; Lamotte, Olivier; Metraux, Jean-Pierre (Public Library of Science, 2011)

[EN] Wounded leaves of Arabidopsis thaliana show transient immunity to Botrytis cinerea, the causal agent of grey mould. Using a fluorescent probe, histological staining and a luminol assay, we now show that reactive oxygen ...
Activator and repressor functions of the Mot3 transcription factor in the osmostress response of Saccharomyces cerevisiae

Martínez Montañés, Fernando Vicente; Rienzo, Alessandro; Poveda Huertes, Daniel; Pascual-Ahuir Giner, María Desamparados; Proft, Markus Hans (American Society for Microbiology, 2013-05)

[EN] Mot3 and Rox1 are transcriptional repressors of hypoxic genes. Both factors recently have been found to be involved in the adaptive response to hyperosmotic stress, with an important function in the adjustment of ...
Analysis of the contribution of the different prefoldin complex subunits to Arabidopsis development and response

Esquinas Ariza, Rosa María (2020-02-12)

[ES] El complejo de prefoldina se identificó originalmente en humanos como co-chaperona que ayuda a la CCT de chaperonina citosólica en el plegamiento de actina y tubulinas. Es un complejo heteromérico formado por seis ...
Análisis de expresión de genes en células tumorales circulantes en tumores del tracto gastrointestinal, mediante desarrollo y validación de un ensayo de multiplexado basado en tecnología xMAP de Luminex

Sánchez Torrecilla, Francisco Javier (2020-07-03)

[ES] En este trabajo se desarrolla y valida un nuevo ensayo para el análisis de la expresión de genes en células tumorales circulantes. Este ensayo se fundamenta en el concepto de biopsia líquida que se basa en buscar ...
Application of genomic tools in plant breeding

Pérez de Castro, Ana María; Vilanova Navarro, Santiago; Cañizares Sales, Joaquín; Pascual Bañuls, Laura; Blanca Postigo, José Miguel; Díez Niclós, Mª José Teresa de Jesús; Prohens Tomás, Jaime; Picó Sirvent, María Belén (Bentham Science Publishers, 2012-05)

[EN] Plant breeding has been very successful in developing improved varieties using conventional tools and methodologies. Nowadays, the availability of genomic tools and resources is leading to a new revolution of plant ...
Bayesian recursive mixed linear model for gene expression analyses with continuous covariates

Casellas, J.; Ibáñez-Escriche, Noelia (American Society of Animal Science, 2012-01)

[EN] The analysis of microarray gene expression data has experienced a remarkable growth in scientific research over the last few years and is helping to decipher the genetic background of several productive traits. ...
Characterization of temperature stress-related gene expression changes in cold-active Shewanella sp. #4 using real-time quantitative polymerase chain reaction (qRT-PCR)

Díaz Pérez, Aurora (2020-07-15)

[EN] Shewanella sp. #4 belongs to the Shewanella genus, which is a unique member of the marine bacteria family Shewanellaceae. They are Gram-negative facultatively anaerobic, rod-shaped bacteria that are found in aquatic ...
Characterization of tomato Cycling Dof Factors reveals conserved and new functions in the control of flowering time and abiotic stress responses

Corrales, Alba-Rocio; González Nebauer, Sergio; Carrillo, Laura; Fernández Nohales, Pedro; Marques Signes, Jorge; Renau Morata, Begoña; Granell, Antonio; Pollmann, Stephan; Vicente-Carbajosa, Jesus; Molina Romero, Rosa Victoria; Medina, Joaquin (Oxford University Press (OUP), 2014-03)

[EN] DNA binding with One Finger (DOF) transcription factors are involved in multiple aspects of plant growth and development but their precise roles in abiotic stress tolerance are largely unknown. Here we report a group ...
Cloning, Sequence Analysis and Expression Patterns during Seed Germination of a Rapeseed (Brassica napus L.) G-x-S-x-G-motif Lipase Gene

Glaied Ghram, Imen; Belguith, Hatem; Ben Mustapha, Maha; Himila, Issam; Bouhaouala, Balkiss; Vicente, Oscar; Ben Hamida, Jeannette (AcademicPres (EAP) Publishing House, 2016)

[EN] Lipases catalyze the hydrolysis of ester bonds in triacylglycerides, generating glycerol and free fatty acids. These enzymes are encoded by extremely complex gene families, and appear to fulfil many different biological ...
Deciphering dynamic dose responses of natural promoters and single cis elements upon osmotic and oxidative stress in yeast

Dolz Edo, Laura; Rienzo, Alessandro; Poveda Huertes, Daniel; Pascual-Ahuir Giner, María Desamparados; Proft, Markus Hans (American Society for Microbiology, 2013-06)

[EN] Fine-tuned activation of gene expression in response to stress is the result of dynamic interactions of transcription factors with specific promoter binding sites. In the study described here we used a time-resolved ...
Development and evaluation of a real-time PCR assay for quantification of Giardia and Cryptosporidium in sewage samples

Alonso Molina, José Luís; Amoros, Inmaculada; Cañigral Cárcel, Irene (SPRINGER, 2011)

[EN] Cryptosporidium and Giardia are major causes of diarrheal disease in humans worldwide and are major causes of protozoan waterborne diseases. Two DNA TaqMan PCR-based Giardia and Cryptosporidium methods targeting a ...
Differential expression in RNA-seq: A matter of depth

Tarazona Campos, Sonia; García-Alcalde, Fernando; Dopazo, Joaquín; Ferrer Riquelme, Alberto José; Conesa, Ana (Cold Spring Harbor Laboratory Press, 2011-09-08)

Next-generation sequencing (NGS) technologies are revolutionizing genome research, and in particular, their application to transcriptomics (RNA-seq) is increasingly being used for gene expression profiling as a replacement ...
Differential gene expression profiles in foetal skin of Rex rabbits with different wool density

Liu, L.; Li, B.; Zhu, Y.L.; Wang, C.Y.; Li, F.C. (Universitat Politècnica de València, 2016-09-28)

[EN] This study investigated the mechanisms controlling hair follicle development in the Rex rabbit. The Agilent rabbit gene expression microarray was used to determine differentially expressed genes in Rex rabbit foetuses ...
Efecto de la dieta sobre la respuesta intestinal a un reto bacteriano en dorada (Sparus aurata, L.): Ensayo in vitro.

Trujillo Martínez, Irene (2018-09-18)

[ES] El creciente coste y limitada disponibilidad de la harina de pescado empleada en las formulaciones de los piensos acuícolas, ha promovido la necesidad de buscar nuevas estrategias, en relación a la dieta de los ...
Efecto de las gonadotropinas recombinantes humanas rhFSH y rhLH sobre el desarrollo embrionario en conejo.

Herreros Pomares, Alejandro (2014-09-19)

[ES] La obtención de un gran número de óvulos y embriones es interesante tanto en la práctica clínica como en la producción animal, pues reduce costes y facilita la mejora y el desarrollo de herramientas reproductivas y ...
Efecto del tiempo de cultivo sobre la viabilidad y desarrollo de embriones de conejo.

Alberola Romano, Ana (2018-09-18)

[ES] El conejo es utilizado como modelo animal en la biología reproductiva debido a las ventajas que suponen ser una especie de ovulación inducida por el coito, de ciclo reproductivo corto, del que es posible obtener, ...
Effect of corifollitropin alfa supplemented with or without LH on ovarian stimulation and embryo viability in rabbit

Viudes De Castro, María Pilar; Marco-Jiménez, Francisco; Cedano-Castro, José Isaí; Vicente Antón, José Salvador (Elsevier, 2017)

[EN] There is increasing interest in using rabbits for research as a laboratory model as well as for industrial production of meat, wool and fur. Superovulation in animals is used to produce a maximum number of transferable ...
Effect of different culture systems on mRNA expression in developing rabbit embryos.

Saenz de Juano Ribes, María de los Desamparados; Naturil Alfonso, Carmen; Vicente Antón, José Salvador; Marco Jiménez, Francisco (Cambridge University Press (CUP), 2013-02)

[EN] The rate of zygotes in vitro developed to hatched blastocyst stage was evaluated between two different commercial media (TCM-199 and Ham's F10) and two different culture systems (renewal and non-renewal single medium) ...