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Browsing by Subject "Isothermal amplification"

Now showing items 1-7 of 7

Fast DNA biosensing based on isothermal amplification, unmodified gold nanoparticles, and smartphone detection

Tortajada-Genaro, Luis Antonio; Lucío, María Isabel; Maquieira Catala, Angel (Elsevier, 2022-07)

[EN] The protection of the consumer against foodborne illnesses and fraudulent practices requires methods capable of detecting critical components. Classical instrumental and DNA-based technologies have assay time, ...
Isothermal-based DNA biosensors for application in pharmacogenetics

Yamanaka, Eric Seiti (Universitat Politècnica de València, 2020-07-21)

[EN] The determination of genetic biomarkers is progressively becoming more extended and popular, being commercialized even in kits for personalized medicine. Establishing specific genotype variations for each patient, ...
Low-cost genotyping method based on allele-specific recombinase polymerase amplification and colorimetric microarray detection

YAMANAKA, ERIC-SEITI; Tortajada-Genaro, Luis Antonio; Maquieira, A. (Springer-Verlag, 2017-05)

[EN] The costs of current genotyping methods limit their application to personalized therapy. The authors describe an alternative approach for the detection of single-point-polymorphisms using recombinant polymerase ...
Magnetic concentration of allele-specific products from recombinase polymerase amplification

Martorell-Tejedor, Sara; Tortajada-Genaro, Luis Antonio; Maquieira Catala, Angel (Elsevier, 2019-12-27)

[EN] The studied challenge is the specific detection of low-abundant genomic variants that differ by a single nucleotide from the wild type. The combination of blocked recombinase polymerase amplification (RPA) and selective ...
Multiple recombinase polymerase amplification and low-cost array technology for the screening of genetically modified organisms

Tortajada-Genaro, Luis Antonio; Maquieira Catala, Ángel (Elsevier, 2021-10)

[EN] The identification of different transgenic products that are potentially present in foods is a priority given their impact on environmental safeness and health care. In this context, reliable, fast and inexpensive ...
Recombinase polymerase and enzyme-linked immunosorbent assay as a DNA amplification-detection strategy for food analysis

Santiago Felipe, Sara; Tortajada-Genaro, Luis Antonio; Puchades, Rosa; Maquieira Catala, Ángel (Elsevier, 2014-02-06)

[EN] Polymerase chain reaction in conjunction with enzyme-linked immunosorbent assay (PCR-ELISA) is a well-established technique that provides a suitable rapid, sensitive, and selective method for a broad range of applications. ...
Surface coupling of oligo-functionalized dendrimers to detect DNA mutations after blocked isothermal amplification

Martorell, Sara; Tortajada-Genaro, Luis Antonio; González Martínez, Miguel Ángel; Maquieira Catala, Ángel (Elsevier, 2021-10)

[EN] This study presents a useful strategy for the bioconjugation of probes for developing high-performance versatile chips applied to fast-response, low-cost DNA biosensing. We herein demonstrate that the high-density ...