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Browsing by Subject "Microspore embryogenesis"

Now showing items 1-20 of 20

A change of developmental program induces the remodeling of the interchromatin domain during microspore embryogenesis in Brassica napus L

Seguí-Simarro, Jose M.; Corral Martínez, Patricia; Corredor, E.; Raska, I.; Testillano, P. S.; Risueno, M.C. (Elsevier, 2011-05-15)

After a stress treatment, in vitro-cultured pollen changes its normal gametophytic developmental pathway towards embryogenesis producing multicellular embryos from which, finally, haploid and double haploid plants develop. ...
Cultivo de anteras y microsporas aisladas con extractos purificados de endospermo líquido de Cocos nucifera

Tur Giménez, Laura (Universitat Politècnica de València, 2020-10-05)

[ES] La embriogénesis de microsporas consiste en desviar in vitro la ruta normal de desarrollo gametofítico de las microsporas para formar un embrión a partir del cual se obtendrán plantas haploides o dobles haploides tras ...
Development and characterization of an eggplant (Solanum melongena) doubled haploid population and a doubled haploid line with high androgenic response

Rivas-Sendra, Alba; Manuel Campos-Vega; Calabuig-Serna, Antonio; Seguí-Simarro, Jose M. (Springer-Verlag, 2017)

[EN] We developed an eggplant doubled haploid (DH) population from a commercial hybrid through androgenesis in microspore culture. Morphological variation, reproductive ability and androgenic responsiveness were evaluated. ...
Effects of growth conditions of donor plants and in vitro culture environment in the viability and the embryogenic response of microspores of different eggplant genotypes

Rivas-Sendra, Alba; Corral Martínez, Patricia; Camacho-Fernández, Carolina; Porcel, R; Seguí-Simarro, Jose M. (Springer-Verlag, 2020-10-06)

[EN] Notwithstanding the importance of eggplant in global horticulture, doubled haploid production in this species is still far from being efficient. Although acknowledged to have a role in the efficiency of androgenesis ...
Efficient production of callus-derived doubled haploids through isolated microspore culture in eggplant (Solanum melongena L.)

Corral Martínez, Patricia; Seguí-Simarro, José M. (Springer Verlag (Germany), 2012)

Production of doubled haploids (DHs) through androgenesis induction is an important biotechnological tool for plant breeding. In some species, DHs are efficiently obtained through embryogenesis from isolated microspore ...
Evaluation of androgenic competence through anther culture in common eggplant and related species

Salas Aragon, Pablo; Prohens Tomás, Jaime; Seguí-Simarro, José M. (Springer Verlag (Germany), 2011)

Anther culture is a convenient technique to obtain androgenic haploid and doubled haploid (DH) plants. In common eggplant (Solanum melongena), this technique has been used to develop DH pure lines for producing uniform F1 ...
Formation and excretion of autophagic plastids (plastolysomes) in Brassica napus embryogenic microspores

Parra Vega, Verónica; Corral Martínez, Patricia; Rivas-Sendra, Alba; Seguí-Simarro, Jose M. (Frontiers Media, 2015-02)

[EN] The change in developmental fate of microspores reprogrammed toward embryogenesis is a complex but fascinating experimental system where microspores undergo dramatic changes derived from the developmental switch. ...
Improved regeneration of eggplant doubled haploids from microspore-derived calli through organogenesis

Rivas Sendra, Alba; Corral Martínez, Patricia; Camacho Fernández, Carolina; Seguí-Simarro, Jose M. (Springer Verlag (Germany), 2015-09)

[EN] Doubled haploid (DH) technology allows for the production of pure lines, useful for plant breeding, through a one-generation procedure that reduces considerably the time and resources needed to produce them. ...
Induction of androgenesis and production of haploid embryos in anther cultures of borage (Borago officinalis L.)

Eshaghi, Zara Chardoli; Abdollahi, Mohammad Reza; Moosavi, Sayyed Saeed; Deljou, Ali; Seguí-Simarro, Jose M. (Springer Verlag (Germany), 2015-08)

[EN] Borage (Borago officinalis L.) is an important medicinal plant with different culinary, pharmaceutical and industrial properties. Unfortunately, there are no published reports on the establishment of protocols to ...
Induction of embryogenesis in Brassica napus microspores produces a callosic subintinal layer and abnormal cell walls with altered levels of callose and cellulose

Parra Vega, Verónica; Corral Martínez, Patricia; Rivas-Sendra, Alba; Seguí-Simarro, Jose M. (Frontiers Media, 2015-11-25)

The induction of microspore embryogenesis produces dramatic changes in different aspects of the cell physiology and structure. Changes at the cell wall level are among the most intriguing and poorly understood. In this ...
Influence of the stage for anther excision and heterostyly in embryogenesis induction from eggplant anther cultures

Salas Aragon, Pablo; Rivas Sendra, Alba; Prohens Tomás, Jaime; Seguí-Simarro, José M. (Springer Verlag (Germany), 2012)

In this work we address two aspects of eggplant flower biology potentially involved on the efficiency of anther culture: the selection of the best floral stage to extract anthers for culture, and the effect of heterostyly ...
Microspore embryogenesis: cell wall dynamics and reprogramming of cell fate

Camacho Fernández, Carolina (Universitat Politècnica de València, 2021-03-08)

[ES] Los dobles haploides son una gran herramienta para la mejora genética de híbridos debido a que se puede alcanzar la homocigosis completa en una sola generación. Entre las técnicas usadas para obtener estas plantas, ...
Multidisciplinary study of the role of calcium in plant in vitro embryogenesis

Calabuig Serna, Antonio (Universitat Politècnica de València, 2023-09-06)

[ES] El calcio (Ca2+) es un catión esencial que juega un papel fundamental en todos los organismos vivos. Desde el punto de vista funcional, el Ca2+ actúa como un segundo mensajero que regula distintos procesos celulares. ...
Novel features of Brassica napus embryogenic microspores revealed by high pressure freezing and freeze substitution: evidence for massive autophagy and excretion-based cytoplasmic cleaning

Corral Martínez, Patricia; Parra Vega, Verónica; Seguí-Simarro, Jose M. (Oxford University Press (OUP): Policy B - Oxford Open Option A, 2013)

[EN] Induction of embryogenesis from isolated microspore cultures is a complex experimental system where microspores undergo dramatic changes in developmental fate. After ~40 years of application of electron microscopy to ...
Obtención de doble haploides en berenjena (Solanum melongena) a partir del cultivo in vitro de microsporas aisladas

Corral Martínez, Patricia (Universitat Politècnica de València, 2011-07-28)

La producción de doble haploides a través del cultivo de microsporas aisladas es una alternativa para obtener líneas puras mucho más eficaz que las técnicas de mejora genética clásica. Esta técnica presenta las ventajas ...
POLLEN: AN AMAZING MODEL SYSTEM TO STUDY A DISPARATE SERIES OF PLANT BIOLOGICAL PROCESSES

Mir Moreno, Ricardo; Vicente, Oscar (University of Agronomic Sciences and Veterinary Medicine of Bucharest, 2021)

[EN] Pollen, the male gametophyte of higher plants, represents an extremely interesting, and peculiar, experimental system for the study of diverse aspects of plant biology, apart from the - 0bvious - research on gametophytic ...
Production of doubled haploid plants from anther cultures of borage (Borago officinalis L.) by the application of chemical and physical stress

Hoveida, Z.S.; Abdollahi, Mohammad Reza; Mirzaie-Asl, Asghar; Moosavi, Sayyed Saeed; Seguí-Simarro, Jose M. (Springer-Verlag, 2017)

[EN] Anther culture can be used as a powerful tool to produce doubled haploid (DH) lines in medicinal plants, thus accelerating breeding programs. In the particular case of borage (Borago officinalis L.), a method to produce ...
Quantitative and qualitative study of endogenous and exogenous growth regulators in eggplant (Solanum melongena) microspore cultures

Calabuig-Serna, Antonio; Camacho-Fernández, Carolina; Mir Moreno, Ricardo; Porcel, R; Carrera, Esther; LOPEZ DIAZ, ISABEL; Seguí-Simarro, Jose M. (Springer-Verlag, 2022-03)

[EN] In eggplant microspore embryogenesis, embryos are produced and then transformed into undifferentiated calli, instead of developing as true embryos. This is the main current bottleneck that precludes this process from ...
Refining the method for eggplant microspore culture: effect of abscisic acid, epibrassinolide, polyethylene glycol, naphthaleneacetic acid, 6-benzylaminopurine and arabinogalactan proteins

Corral Martínez, Patricia; Seguí-Simarro, Jose M. (Springer Verlag (Germany), 2014-02)

Microspore embryogenesis is an inducible pathway interesting from basic and applied perspectives. For plant breeding, it is a powerful tool to produce doubled haploids, useful as pure lines. The most efficient way to produce ...
Stress treatments and in vitro culture conditions influence microspore embryogenesis and growth of callus from anther walls of sweet pepper (Capsicum annuum L.)

Parra Vega, Verónica; Renau Morata, Begoña; Sifres Cuerda, Alicia Gemma; Seguí Simarro, José María (Springer Verlag (Germany), 2013-03)

Production of doubled haploids (DHs) is a convenient tool to obtain pure lines for breeding purposes. Until now, the easiest and most useful approach to obtain pepper DHs is via anther culture. However, this method has an ...