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Browsing by Subject "Small RNA"

Now showing items 1-8 of 8

Antiviral roles of plant ARGONAUTES

CARBONELL, ALBERTO; Carrington, James C. (Elsevier, 2015-10)

[EN] ARGONAUTES (AGOs) are the effector proteins functioning in eukaryotic RNA silencing pathways. AGOs associate with small RNAs and are programmed to target complementary RNA or DNA. Plant viruses induce a potent and ...
Argonaute PIWI domain and microRNA duplex structure contribute to small RNA sorting in Arabidopsis.

Zhang, Xiaoming; Niu, DongDong; CARBONELL, ALBERTO; Wang, Airong; Lee, Angel; Tun, Vinnary; Wang, Zonghua; Carrington, James C.; Chang, Chia-en A.; Jin, Hailing (Nature Publishing Group, 2014-11-16)

[EN] Small RNAs (sRNAs) are loaded into ARGONAUTE (AGO) proteins to induce gene silencing. In plants, the 5¿-terminal nucleotide is important for sRNA sorting into different AGOs. Here we show that microRNA (miRNA) duplex ...
Artificial microRNAs and synthetic trans-acting small interfering RNAs interfere with viroid infection

Carbonell Olivares, Alberto; Daros Arnau, Jose Antonio (Wiley, 2016)

[EN] Artificial microRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs) are two classes of artificial small RNAs (sRNAs) engineered to silence endogenous transcripts as well as viral RNAs ...
Describing the structural robustness landscape of bacterial small RNAs

Rodrigo Tarrega, Guillermo; Fares Riaño, Mario Ali (BioMed Central, 2012-04-13)

Background: The potential role of RNA molecules as gene expression regulators has led to a new perspective on the intracellular control and genome organization. Because secondary structures are crucial for their regulatory ...
Exploring the Dynamics and Mutational Landscape of Riboregulation with a Minimal Synthetic Circuit in Living Cells

Rodrigo Tarrega, Guillermo; Majer, Eszter; Prakash, Satya; Daros Arnau, Jose Antonio; Jaramillo, Alfonso; Poyatos, Juan F. (Biophysical Society, 2015-09-01)

[EN] The regulation of gene expression, triggered by conformational changes in RNA molecules, is widely observed in cellular systems. Here, we examine this mode of control by means of a model-based design and construction ...
Fast-forward generation of effective artificial small RNAs for enhanced antiviral defense in plants

CARBONELL, ALBERTO; Carrington, James C.; Daros Arnau, Jose Antonio (Smart Science and Technology, 2016-01-12)

[EN] Artificial small RNAs (sRNAs) are short ≈21-nt non-coding RNAs engineered to inactivate sequence complementary RNAs. In plants, they have been extensively used to silence cellular transcripts in gene function analyses ...
Immunoprecipitation and High–Throughput Sequencing of ARGONAUTE–Bound Target RNAs from Plants

Carbonell Olivares, Alberto (Springer Link, 2017-06-13)

ARGONAUTE (AGO) proteins function in small RNA (sRNA)-based RNA silencing pathways to regulate gene expression and control invading nucleic acids. In posttranscriptional RNA silencing pathways, plant AGOs associate with ...
Preparation of multiplexed small RNA libraries from plants

Gilbert, Kerrigan B.; Fahlgren, Noah; Kasschau, Kristin D.; Chapman, Elisabeth J.; Carrington, James C.; CARBONELL, ALBERTO (Bio-Protocol, 2014-11-05)

[EN] High-throughput sequencing is a powerful tool for exploring small RNA populations in plants. The ever-increasing output from an Illumina Sequencing System allows for multiplexing multiple samples while still obtaining ...