Rios, P.; Argyris, J.; Vegas, J.; Leida, C.; Kenigswald, M.; Tzuri, G.; Troadec, C.... (2017). ETHQV6.3 is involved in melon climacteric fruit ripening and is encoded by a NAC domain transcription factor. The Plant Journal. 91(4):671-683. https://doi.org/10.1111/tpj.13596
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/102701
Title:
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ETHQV6.3 is involved in melon climacteric fruit ripening and is encoded by a NAC domain transcription factor
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Author:
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RIOS, P
Argyris, J
VEGAS, J
LEIDA, C
Kenigswald, M
Tzuri, G
Troadec, C
Bendahmane, A
KATZIR, NURIT
Picó Sirvent, María Belén
Monforte Gilabert, Antonio José
GARCÍA-MAS, J
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UPV Unit:
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Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes
Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia
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Issued date:
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Abstract:
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[EN] Fruit ripening is divided into climacteric and non-climacteric types depending on the presence or absence of a transient rise in respiration rate and the production of autocatalytic ethylene. Melon is ideal for the ...[+]
[EN] Fruit ripening is divided into climacteric and non-climacteric types depending on the presence or absence of a transient rise in respiration rate and the production of autocatalytic ethylene. Melon is ideal for the study of fruit ripening, as both climacteric and non-climacteric varieties exist. Two introgressions of the non-climacteric accession PI 161375, encompassed in the QTLs ETHQB3.5 and ETHQV6.3, into the non-climacteric 'Piel de Sapo' background are able to induce climacteric ripening independently. We report that the gene underlying ETHQV6.3 is MELO3C016540 (CmNAC-NOR), encoding a NAC ((N) under bar AM, (A) under bar TAF1,2, (C) under bar UC2) transcription factor that is closely related to the tomato NOR (non-ripening) gene. CmNAC-NOR was functionally validated through the identification of two TILLING lines carrying non-synonymous mutations in the conserved NAC domain region. In an otherwise highly climacteric genetic background, both mutations provoked a significant delay in the onset of fruit ripening and in the biosynthesis of ethylene. The PI 161375 allele of ETHQV6.3 is similar to that of climacteric lines of the cantalupensis type and, when introgressed into the non-climacteric 'Piel de Sapo', partially restores its climacteric ripening capacity. CmNAC-NOR is expressed in fruit flesh of both climacteric and non-climacteric lines, suggesting that the causal mutation may not be acting at the transcriptional level. The use of a comparative genetic approach in a species with both climacteric and non-climacteric ripening is a powerful strategy to dissect the complex mechanisms regulating the onset of fruit ripening.
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Subjects:
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Non-climacteric ripening
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ETHQV6.3
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NAC transcription factor
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TILLING mutant
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Cucumis melo
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Introgression line
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Copyrigths:
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Reserva de todos los derechos
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Source:
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The Plant Journal. (issn:
0960-7412
)
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DOI:
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10.1111/tpj.13596
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Publisher:
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Blackwell Publishing
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Publisher version:
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https://doi.org/10.1111/tpj.13596
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Project ID:
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info:eu-repo/grantAgreement/MINECO//AGL2015-64625-C2-2-R/ES/EVOLUCION Y DIVERSIFICACION EN CUCUMIS. GENETICA DE LA DOMESTICACION, MORFOLOGIA DE FRUTO Y BARRERAS REPRODUCTIVAS/
info:eu-repo/grantAgreement/EC/FP7/341076/EU/Unraveling sex determination and parthenocarpy mechanisms to improve crops/
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Thanks:
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The authors thank Dr J. Burger for providing the NAxDul population, Dr E. Falik for ethylene analysis of this population, and both for their critical inputs. This work was supported by the Spanish Ministry of Economy and ...[+]
The authors thank Dr J. Burger for providing the NAxDul population, Dr E. Falik for ethylene analysis of this population, and both for their critical inputs. This work was supported by the Spanish Ministry of Economy and Competitivity grant AGL2015-64625-C2-1-R, Centro de Excelencia Severo Ochoa 2016-2020, and the CERCA Programme/Generalitat de Catalunya to JGM; Spanish Ministry of Economy and Competitivity/FEDER grant AGL2015-64625-C2-2-R to AJM; EU Framework Program Horizon 2020 COST Action FA1106 Quality Fruit for networking activities to CL; European Research Council grant ERC-SEXYPARTH to AB; Chief Scientist of the Ministry of Agriculture of Israel grant no. 261-1049-13 to NK.
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Type:
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Artículo
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