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Label-Free Pyrophosphate Recognition with Functionalized Asymmetric Nanopores

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Label-Free Pyrophosphate Recognition with Functionalized Asymmetric Nanopores

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dc.contributor.author Ali, Mubarak es_ES
dc.contributor.author Ahmed, Ishtiaq es_ES
dc.contributor.author Ramirez Hoyos, Patricio es_ES
dc.contributor.author Nasir, Saima es_ES
dc.contributor.author Niemeyer, Christof M. es_ES
dc.contributor.author Mafe, Salvador es_ES
dc.contributor.author Ensinger, Wolfgang es_ES
dc.date.accessioned 2018-07-16T06:45:18Z
dc.date.available 2018-07-16T06:45:18Z
dc.date.issued 2016 es_ES
dc.identifier.issn 1613-6810 es_ES
dc.identifier.uri http://hdl.handle.net/10251/105813
dc.description.abstract [EN] The label¿free detection of pyrophosphate (PPi) anions with a nanofluidic sensing device based on asymmetric nanopores is demonstrated. The pore surface is functionalized with zinc complexes based on two di(2¿picolyl)amine [bis(DPA)] moieties using carbodiimide coupling chemistry. The complexation of zinc (Zn2+) ion is achieved by exposing the modified pore to a solution of zinc chloride to form bis(Zn2+¿DPA) complexes. The chemical functionalization is demonstrated by recording the changes in the observed current¿voltage (I¿V) curves before and after pore modification. The bis(Zn2+¿DPA) complexes on the pore walls serve as recognition sites for pyrophosphate anion. The experimental results show that the proposed nanofluidic sensor has the ability to sense picomolar concentrations of PPi anion in the surrounding environment. On the contrary, it does not respond to other phosphate anions, including monohydrogen phosphate, dihydrogen phosphate, adenosine monophosphate, adenosine diphosphate, and adenosine triphosphate. The experimental results are described theoretically by using a model based on the Poisson¿Nernst¿Planck equations. es_ES
dc.description.sponsorship M.A., S.N., and W.E. acknowledge the funding from the Hessen State Ministry of Higher Education, Research and the Arts, Germany, under the LOEWE project iNAPO. P.R. and S.M. acknowledge financial support by the Generalitat Valenciana (Program of Excellence Prometeo/GV/0069), the Spanish Ministry of Economic Affairs and Competitiveness (MAT2015-65011-P), and FEDER. I. A. and C.M.N. acknowledge financial support through the Helmholtz programme BioInterfaces in Technology and Medicine. The authors are also thankful to Prof. C. Trautmann, Department of Materials Research from GSI, for support with irradiation experiments. en_EN
dc.language Inglés es_ES
dc.publisher John Wiley & Sons es_ES
dc.relation MINECO/MAT2015-65011-P es_ES
dc.relation GV/PROMETEU/2012/069 es_ES
dc.relation.ispartof Small es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.title Label-Free Pyrophosphate Recognition with Functionalized Asymmetric Nanopores es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1002/smll.201600160 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.description.bibliographicCitation Ali, M.; Ahmed, I.; Ramirez Hoyos, P.; Nasir, S.; Niemeyer, CM.; Mafe, S.; Ensinger, W. (2016). Label-Free Pyrophosphate Recognition with Functionalized Asymmetric Nanopores. Small. 12(15):2014-2021. doi:10.1002/smll.201600160 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1002/smll.201600160 es_ES
dc.description.upvformatpinicio 2014 es_ES
dc.description.upvformatpfin 2021 es_ES
dc.type.version info:eu repo/semantics/publishedVersion es_ES
dc.description.volume 12 es_ES
dc.description.issue 15 es_ES
dc.relation.pasarela 314418 es_ES
dc.contributor.funder Generalitat Valenciana (GV) es_ES
dc.contributor.funder Ministerio de Economía, Industria y Competitividad (MINECO) es_ES


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