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dc.contributor.author | García -Laínez, Guillermo | es_ES |
dc.contributor.author | Ana M Marínez-Reig | es_ES |
dc.contributor.author | Limones Herrero, Daniel | es_ES |
dc.contributor.author | Jiménez Molero, María Consuelo | es_ES |
dc.contributor.author | Miranda Alonso, Miguel Ángel | es_ES |
dc.contributor.author | Andreu Ros, María Inmaculada | es_ES |
dc.date.accessioned | 2018-07-16T06:46:34Z | |
dc.date.available | 2018-07-16T06:46:34Z | |
dc.date.issued | 2018 | es_ES |
dc.identifier.issn | 0041-008X | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/105816 | |
dc.description.abstract | [EN] Diclofenac (DCF) can cause adverse reactions such as gastrointestinal, renal and cardiovascular disorders; therefore, topical administration may be an attractive alternative to the management of local pain in order to avoid these side effects. However, previous studies have shown that DCF, in combination with sunlight, displays capability to induce photosensitivity disorders. In humans, DCF is biotransformed into hydroxylated metabolites at positions 4¿ and 5 (4¿OH-DCF and 5OH-DCF), and this chemical change produces non negligible alterations of the drug chromophore, resulting in a significant modification of its light-absorbing properties. In the present work, 5OH-DCF exhibited higher photo(geno)toxic potential than the parent drug, as shown by several in vitro assays (3T3 NRU phototoxicity, DNA ssb gel electrophoresis and COMET), whereas 4¿OH-DCF did not display significant photo(geno)toxicity. This could be associated, at least partially with their more efficient UV-light absorption by 5OH-DCF metabolite and with a higher photoreactivity. Interestingly, most of the cellular DNA damage photosensitized by DCF and 5OH-DCF was repaired by the cells after several hours, although this effect was not complete in the case of 5OH-DCF. | es_ES |
dc.description.sponsorship | This work was supported by the Carlos III Institute of Health (Grants: RD16/0006/0030, PI16/01877), by the MINECO (Grants: CTQ2013-47872, CTQ2016-78875), and by the Generalitat Valenciana (Prometeo 2017/075). | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation.ispartof | Toxicology and Applied Pharmacology | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Comet assay | es_ES |
dc.subject | DNA repair capability, Metabolites | es_ES |
dc.subject | Photosensitized DNA damage | es_ES |
dc.subject | Phototoxicity | es_ES |
dc.subject.classification | QUIMICA ORGANICA | es_ES |
dc.subject.classification | QUIMICA ANALITICA | es_ES |
dc.title | Photo(geno)toxicity changes associated with hydroxylation of the aromatic chromophores during diclofenac metabolism | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1016/j.taap.2018.01.005 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//RD16%2F0006%2F0030/ES/Asma, Reacciones Adversas y Alérgicas (ARADYAL)/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//CTQ2016-78875-P/ES/CONTROL SUPRAMOLECULAR DE LA FOTORREACTIVIDAD EN MEDIOS MICROHETEROGENOS BASADOS EN AMINOACIDOS: GELES MOLECULARES Y PROTEINAS TRANSPORTADORAS COMO NANORREACTORES/ | |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//PROMETEO%2F2017%2F075/ES/Reacciones fotoquímicas de biomoléculas/ | |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//CTQ2013-47872-C2-1-P/ES/METABOLITOS FOTOACTIVOS/ | |
dc.rights.accessRights | Abierto | es_ES |
dc.date.embargoEndDate | 2019-02-15 | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Tecnología Química - Institut Universitari Mixt de Tecnologia Química | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Química - Departament de Química | es_ES |
dc.description.bibliographicCitation | García -Laínez, G.; Ana M Marínez-Reig; Limones Herrero, D.; Jiménez Molero, MC.; Miranda Alonso, MÁ.; Andreu Ros, MI. (2018). Photo(geno)toxicity changes associated with hydroxylation of the aromatic chromophores during diclofenac metabolism. Toxicology and Applied Pharmacology. 341:51-55. https://doi.org/10.1016/j.taap.2018.01.005 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.taap.2018.01.005 | es_ES |
dc.description.upvformatpinicio | 51 | es_ES |
dc.description.upvformatpfin | 55 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 341 | es_ES |
dc.identifier.pmid | 29325823 | |
dc.relation.pasarela | S\355290 | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad | es_ES |
dc.contributor.funder | Ministerio de Economía, Industria y Competitividad |