Gamboa Martínez, TC.; Ródenas Rochina, J.; Rico Tortosa, PM.; Lebourg, MM.; Gómez Ribelles, JL.; Salmerón Sánchez, M.; Gallego Ferrer, G. (2013). Chondrocytes cultured in an adhesive macroporous scaffold subjected to stirred flow bioreactor behave like in static culture. Journal of Biomaterials and Tissue Engineering. 3(3):312-319. https://doi.org/10.1166/jbt.2013.1091
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/105869
Title:
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Chondrocytes cultured in an adhesive macroporous scaffold subjected to stirred flow bioreactor behave like in static culture
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Author:
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Gamboa Martínez, Tatiana Carolina
Ródenas Rochina, Joaquín
Rico Tortosa, Patricia María
Lebourg, Myriam Madeleine
Gómez Ribelles, José Luís
Salmerón Sánchez, Manuel
Gallego Ferrer, Gloria
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UPV Unit:
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Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada
Universitat Politècnica de València. Centro de Biomateriales e Ingeniería Tisular - Centre de Biomaterials i Enginyeria Tissular
Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada
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Issued date:
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Abstract:
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[EN] The aim of this study was to evaluate whether mechanical stimulation helps to modulate the redifferentiation of human chondrocytes on adhesive 3D substrates. In a previous work, it was shown that chondrocytes seeded ...[+]
[EN] The aim of this study was to evaluate whether mechanical stimulation helps to modulate the redifferentiation of human chondrocytes on adhesive 3D substrates. In a previous work, it was shown that chondrocytes seeded in a non-adherent porous scaffold cluster inside the pores when cultured in a bioreactor and produced extracellular matrix with the components of hyaline cartilage suggesting cellular redifferentiation. The current study followed the same culture protocol for up to 4 weeks using two substrates with good adhesion properties: polycaprolactone (PCL) scaffolds uncoated, and coated with fibrin. The cellular response was characterized by studying the cell-material interaction at short times (6, 24 and 72 h), followed by the use of a biological stirrer to evaluate its effect on the chondrogenesis for 7, 14 and 28 days. The study showed strong cell-substrate interaction in both coated and uncoated scaffolds. In short times, cells adhere to the pore walls, form focal adhesions, develop actin fibers and acquire spread morphology showing the characteristics of a monolayer culture. The use of a bioreactor system was useful for reducing cellular proliferation for up to 14 days but no signs of redifferentiation were found for long culture times.
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Subjects:
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Bioreactor
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Chondrocytes
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Fibrin coating
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Polycaprolactone
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Redifferentiation
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Scaffold
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Copyrigths:
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Cerrado |
Source:
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Journal of Biomaterials and Tissue Engineering. (issn:
2157-9083
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DOI:
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10.1166/jbt.2013.1091
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Publisher:
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American Scientific Publishers
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Publisher version:
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https://doi.org/10.1166/jbt.2013.1091
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Project ID:
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info:eu-repo/grantAgreement/MICINN//DPI2010-20399-C04-03/ES/DISEÑO Y FABRICACION DE UNA PLATAFORMA BIOMIMETICA TIPO SCAFFOLD%2FSOPORTE PARA LA REGENERACION DEL CARTILAGO ARTICULAR/
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Thanks:
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The authors are grateful for the financial support received from the Spanish Ministry through the DPI2010-20399-004-03 project. CIBER-BBN is an initiative funded by the VI National R&D&i Plan 2008-2011, Iniciativa Ingenio ...[+]
The authors are grateful for the financial support received from the Spanish Ministry through the DPI2010-20399-004-03 project. CIBER-BBN is an initiative funded by the VI National R&D&i Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program. CIBER Actions are financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. We would also like to thank Dr. N. Garcia-Giralt from the Institut Municipal d'Investigacio Medica de l'Hospital del Mar, Barcelona, for supplying chondrocytes.
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Type:
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Artículo
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