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Use of fluorescent in situ hybridization to evidence the presence of Helicobacter pylori in water

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Use of fluorescent in situ hybridization to evidence the presence of Helicobacter pylori in water

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dc.contributor.author Moreno Trigos, Mª Yolanda es_ES
dc.contributor.author Ferrús Pérez, Mª Antonia es_ES
dc.contributor.author Alonso Molina, José Luís es_ES
dc.contributor.author Jiménez Belenguer, Ana Isabel es_ES
dc.contributor.author Hernández Haba, Javier es_ES
dc.date.accessioned 2019-03-23T21:01:31Z
dc.date.available 2019-03-23T21:01:31Z
dc.date.issued 2003 es_ES
dc.identifier.issn 0043-1354 es_ES
dc.identifier.uri http://hdl.handle.net/10251/118455
dc.description.abstract [EN] We have evaluated the use of a fluorescent in situ hybridization (FISH) technique for the detection of Helicobacter pylori in water (river and wastewater) samples. The assay was compared with PCR detection and isolation of cells on selective media. 16S rRNA and UreA+B sequence data were used as oligonucleotide probe and specific primers for FISH and PCR, respectively. Using FISH technique, H. pylori was detected in two river water and one wastewater samples, while PCR yielded only one positive result. H. pylori culture was not possible from any sample. According to these results, FISH technique has the potential to be used as a quick and sensitive method for detection of H. pylori in environmental samples. es_ES
dc.description.sponsorship We thank E. Echevarrı́a from EMARSA company, which allowed us access and sampling Pinedo wastewater treatment plant.
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Water Research es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject Helicobacter es_ES
dc.subject PCR es_ES
dc.subject FishWater es_ES
dc.subject Activated sludge es_ES
dc.subject Detection es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.title Use of fluorescent in situ hybridization to evidence the presence of Helicobacter pylori in water es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/S0043-1354(02)00624-3 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Ingeniería del Agua y del Medio Ambiente - Institut Universitari d'Enginyeria de l'Aigua i Medi Ambient es_ES
dc.description.bibliographicCitation Moreno Trigos, MY.; Ferrús Pérez, MA.; Alonso Molina, JL.; Jiménez Belenguer, AI.; Hernández Haba, J. (2003). Use of fluorescent in situ hybridization to evidence the presence of Helicobacter pylori in water. Water Research. 37:2251-2256. https://doi.org/10.1016/S0043-1354(02)00624-3 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/:10.1016/S0043-1354(02)00624-3 es_ES
dc.description.upvformatpinicio 2251 es_ES
dc.description.upvformatpfin 2256 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 37 es_ES
dc.identifier.pmid 12691913
dc.relation.pasarela S\23845 es_ES


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