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dc.contributor.author | Moreno Trigos, Mª Yolanda | es_ES |
dc.contributor.author | Ferrús Pérez, Mª Antonia | es_ES |
dc.contributor.author | Alonso Molina, José Luís | es_ES |
dc.contributor.author | Jiménez Belenguer, Ana Isabel | es_ES |
dc.contributor.author | Hernández Haba, Javier | es_ES |
dc.date.accessioned | 2019-03-23T21:01:31Z | |
dc.date.available | 2019-03-23T21:01:31Z | |
dc.date.issued | 2003 | es_ES |
dc.identifier.issn | 0043-1354 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/118455 | |
dc.description.abstract | [EN] We have evaluated the use of a fluorescent in situ hybridization (FISH) technique for the detection of Helicobacter pylori in water (river and wastewater) samples. The assay was compared with PCR detection and isolation of cells on selective media. 16S rRNA and UreA+B sequence data were used as oligonucleotide probe and specific primers for FISH and PCR, respectively. Using FISH technique, H. pylori was detected in two river water and one wastewater samples, while PCR yielded only one positive result. H. pylori culture was not possible from any sample. According to these results, FISH technique has the potential to be used as a quick and sensitive method for detection of H. pylori in environmental samples. | es_ES |
dc.description.sponsorship | We thank E. Echevarrı́a from EMARSA company, which allowed us access and sampling Pinedo wastewater treatment plant. | |
dc.language | Inglés | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation.ispartof | Water Research | es_ES |
dc.rights | Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) | es_ES |
dc.subject | Helicobacter | es_ES |
dc.subject | PCR | es_ES |
dc.subject | FishWater | es_ES |
dc.subject | Activated sludge | es_ES |
dc.subject | Detection | es_ES |
dc.subject.classification | MICROBIOLOGIA | es_ES |
dc.title | Use of fluorescent in situ hybridization to evidence the presence of Helicobacter pylori in water | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1016/S0043-1354(02)00624-3 | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario de Ingeniería del Agua y del Medio Ambiente - Institut Universitari d'Enginyeria de l'Aigua i Medi Ambient | es_ES |
dc.description.bibliographicCitation | Moreno Trigos, MY.; Ferrús Pérez, MA.; Alonso Molina, JL.; Jiménez Belenguer, AI.; Hernández Haba, J. (2003). Use of fluorescent in situ hybridization to evidence the presence of Helicobacter pylori in water. Water Research. 37:2251-2256. https://doi.org/10.1016/S0043-1354(02)00624-3 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://doi.org/:10.1016/S0043-1354(02)00624-3 | es_ES |
dc.description.upvformatpinicio | 2251 | es_ES |
dc.description.upvformatpfin | 2256 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 37 | es_ES |
dc.identifier.pmid | 12691913 | |
dc.relation.pasarela | S\23845 | es_ES |