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dc.contributor.author | Lázaro-Zaragozá, Ana | es_ES |
dc.contributor.author | Yamanaka, Eric-Seiti | es_ES |
dc.contributor.author | Maquieira Catala, Angel | es_ES |
dc.contributor.author | Tortajada-Genaro, Luis Antonio | es_ES |
dc.date.accessioned | 2020-04-24T07:13:20Z | |
dc.date.available | 2020-04-24T07:13:20Z | |
dc.date.issued | 2019 | es_ES |
dc.identifier.issn | 0925-4005 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/141435 | |
dc.description.abstract | [EN] A novel multiplex detection of single nucleotide polymorphisms (SNPs), with point-of-care testing as its aim, is reported for supporting pharmacogenetic-based decisions. The strategy relies on allele-specific ligation to discriminate base sequence variations at the SNP site and the extension of generated products by isothermal amplification and recombinase polymerase amplification (RPA). Having demonstrated the assay principle, the variables for the adequate integration of the ligation-amplification process were studied and compared to a conventional PCR approach. One key result was the development of RPA in a universal format using short-length primers, which enabled detection based on selective hybridisation on a barcode-DNA chip and a low-cost optical sensor. As proof of concept, we successfully discriminated genetic variants related to cardiovascular diseases and the adequate prescription of oral anticoagulant antagonists of vitamin K (genes CYP2C9 and VKROC1). | es_ES |
dc.description.sponsorship | The authors acknowledge the financial support received from the Generalitat Valenciana (GRISOLIA/2014/024PhD Grant and GVA-FPI-2017PhD Grant) and the Spanish Ministry of Economy and Competitiveness (MINECO Project CTQ2016-75749-R). | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation.ispartof | Sensors and Actuators B Chemical | es_ES |
dc.rights | Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) | es_ES |
dc.subject | Universal RPA | es_ES |
dc.subject | Ligation | es_ES |
dc.subject | SNP | es_ES |
dc.subject | Optical sensor | es_ES |
dc.subject | Hybridization chip | es_ES |
dc.subject | Personalised healthcare | es_ES |
dc.subject.classification | QUIMICA ANALITICA | es_ES |
dc.title | Allele-specific ligation and recombinase polymerase amplification for the detection of single nucleotide polymorphisms | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1016/j.snb.2019.126877 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//GRISOLIA%2F2014%2F024/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//ACIF%2F2017%2F031/ES/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//CTQ2016-75749-R/ES/BIOSENSORES HOLOGRAFICOS. PRUEBA DE CONCEPTO Y DEMOSTRACION EN APLICACIONES CLINICAS/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Química - Departament de Química | es_ES |
dc.description.bibliographicCitation | Lázaro-Zaragozá, A.; Yamanaka, E.; Maquieira Catala, A.; Tortajada-Genaro, LA. (2019). Allele-specific ligation and recombinase polymerase amplification for the detection of single nucleotide polymorphisms. Sensors and Actuators B Chemical. 298. https://doi.org/10.1016/j.snb.2019.126877 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.snb.2019.126877 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 298 | es_ES |
dc.relation.pasarela | S\392797 | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad | es_ES |