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A collection of enhancer trap insertional mutants for functional genomics in tomato

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A collection of enhancer trap insertional mutants for functional genomics in tomato

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dc.contributor.author Pérez-Martín, Fernando es_ES
dc.contributor.author Yuste-Lisbona, Fernando J. es_ES
dc.contributor.author Pineda Chaza, Benito José es_ES
dc.contributor.author Angarita-Díaz, María Pilar es_ES
dc.contributor.author García Sogo, Begoña es_ES
dc.contributor.author Antón Martínez, María Teresa es_ES
dc.contributor.author SANCHEZ MARTÍN-SAUCEDA, SIBILLA es_ES
dc.contributor.author Gimenez, Estela es_ES
dc.contributor.author Atarés Huerta, Alejandro es_ES
dc.contributor.author Fernández-Lozano, Antonia es_ES
dc.contributor.author Ortiz-Atienza, Ana es_ES
dc.contributor.author García-Alcázar, Manuel es_ES
dc.contributor.author Castañeda, Laura es_ES
dc.contributor.author Fonseca, Rocio es_ES
dc.contributor.author Capel, Carmen es_ES
dc.contributor.author Goergen, Geraldine es_ES
dc.contributor.author Sanchez-Lopez, Jorge es_ES
dc.contributor.author Quispe, Jorge L. es_ES
dc.contributor.author Capel, Juan es_ES
dc.contributor.author Angosto, Trinidad es_ES
dc.contributor.author Moreno Ferrero, Vicente es_ES
dc.contributor.author Lozano, Rafael es_ES
dc.date.accessioned 2020-10-28T04:32:47Z
dc.date.available 2020-10-28T04:32:47Z
dc.date.issued 2017 es_ES
dc.identifier.issn 1467-7644 es_ES
dc.identifier.uri http://hdl.handle.net/10251/153362
dc.description.abstract [EN] With the completion of genome sequencing projects, the next challenge is to close the gap between gene annotation and gene functional assignment. Genomic tools to identify gene functions are based on the analysis of phenotypic variations between a wild type and its mutant; hence, mutant collections are a valuable resource. In this sense, T-DNA collections allow for an easy and straightforward identification of the tagged gene, serving as the basis of both forward and reverse genetic strategies. This study reports on the phenotypic and molecular characterization of an enhancer trap T-DNA collection in tomato (Solanum lycopersicum L.), which has been produced by Agrobacterium-mediated transformation using a binary vector bearing a minimal promoter fused to the uidA reporter gene. Two genes have been isolated from different T-DNA mutants, one of these genes codes for a UTP-glucose-1-phosphate uridylyltransferase involved in programmed cell death and leaf development, which means a novel gene function reported in tomato. Together, our results support that enhancer trapping is a powerful tool to identify novel genes and regulatory elements in tomato and that this T-DNA mutant collection represents a highly valuable resource for functional analyses in this fleshy-fruited model species. es_ES
dc.description.sponsorship This work was supported by research grants from the Spanish Ministerio de Economia y Competitividad (AGL2012-40150-C02-01, AGL2012-40150-C02-02, AGL2015-64991-C3-1-R and AGL2015-64991-C3-3-R), Junta de Andalucia (P10-AGR-6931) and UE-FEDER. B.P. received a JAE-Doc research contract from the CSIC (Spain). PhD fellowships were funded by the FPU (M.G-A. and R.F.) and the FPI (M.P.A., S.S.A. F-L., A.O-A and L.C.) Programmes of the Ministerio de Ciencia e Innovacion, the JAE predoc Programme of the Spanish CSIC (G.G.), the CONACYT and Universidad de Sinaloa of Mexico (J.S.) and the LASPAU (J.L.Q.). The authors thank research facilities provided by the Campus de Excelencia Internacional Agroalimentario (CeiA3) es_ES
dc.language Inglés es_ES
dc.publisher Blackwell Publishing es_ES
dc.relation.ispartof Plant Biotechnology Journal es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject T-DNA es_ES
dc.subject Enhancer trapping es_ES
dc.subject Solanum lycopersicum es_ES
dc.subject Functional genomics es_ES
dc.subject Insertional mutagenesis es_ES
dc.subject GUS expression es_ES
dc.subject.classification GENETICA es_ES
dc.title A collection of enhancer trap insertional mutants for functional genomics in tomato es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/pbi.12728 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/Junta de Andalucía//P10-AGR-6931/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2012-40150-C03-01/ES/IDENTIFICACION, ETIQUETADO Y ANALISIS FUNCIONAL DE GENES IMPLICADOS EN EL CUAJADO DEL FRUTO DE TOMATE Y TOLERANCIA A LA SALINIDAD EN ESPECIES SILVESTRES RELACIONADAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2015-64991-C3-3-R/ES/GENOMICA FUNCIONAL Y MEJORA GENETICA DE TOMATE: IMPORTANCIA AGRONOMICA DEL BALANCE DESARROLLO - ESTRES ABIOTICO/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Pérez-Martín, F.; Yuste-Lisbona, FJ.; Pineda Chaza, BJ.; Angarita-Díaz, MP.; García Sogo, B.; Antón Martínez, MT.; Sanchez Martín-Sauceda, S.... (2017). A collection of enhancer trap insertional mutants for functional genomics in tomato. Plant Biotechnology Journal. 15(11):1439-1452. https://doi.org/10.1111/pbi.12728 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1111/pbi.12728 es_ES
dc.description.upvformatpinicio 1439 es_ES
dc.description.upvformatpfin 1452 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 15 es_ES
dc.description.issue 11 es_ES
dc.relation.pasarela S\351693 es_ES
dc.contributor.funder Junta de Andalucía es_ES
dc.contributor.funder Consejo Superior de Investigaciones Científicas es_ES
dc.contributor.funder Consejo Nacional de Ciencia y Tecnología, México es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES


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