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dc.contributor.author | Pérez-Martín, Fernando | es_ES |
dc.contributor.author | Yuste-Lisbona, Fernando J. | es_ES |
dc.contributor.author | Pineda Chaza, Benito José | es_ES |
dc.contributor.author | Angarita-Díaz, María Pilar | es_ES |
dc.contributor.author | García Sogo, Begoña | es_ES |
dc.contributor.author | Antón Martínez, María Teresa | es_ES |
dc.contributor.author | SANCHEZ MARTÍN-SAUCEDA, SIBILLA | es_ES |
dc.contributor.author | Gimenez, Estela | es_ES |
dc.contributor.author | Atarés Huerta, Alejandro | es_ES |
dc.contributor.author | Fernández-Lozano, Antonia | es_ES |
dc.contributor.author | Ortiz-Atienza, Ana | es_ES |
dc.contributor.author | García-Alcázar, Manuel | es_ES |
dc.contributor.author | Castañeda, Laura | es_ES |
dc.contributor.author | Fonseca, Rocio | es_ES |
dc.contributor.author | Capel, Carmen | es_ES |
dc.contributor.author | Goergen, Geraldine | es_ES |
dc.contributor.author | Sanchez-Lopez, Jorge | es_ES |
dc.contributor.author | Quispe, Jorge L. | es_ES |
dc.contributor.author | Capel, Juan | es_ES |
dc.contributor.author | Angosto, Trinidad | es_ES |
dc.contributor.author | Moreno Ferrero, Vicente | es_ES |
dc.contributor.author | Lozano, Rafael | es_ES |
dc.date.accessioned | 2020-10-28T04:32:47Z | |
dc.date.available | 2020-10-28T04:32:47Z | |
dc.date.issued | 2017 | es_ES |
dc.identifier.issn | 1467-7644 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/153362 | |
dc.description.abstract | [EN] With the completion of genome sequencing projects, the next challenge is to close the gap between gene annotation and gene functional assignment. Genomic tools to identify gene functions are based on the analysis of phenotypic variations between a wild type and its mutant; hence, mutant collections are a valuable resource. In this sense, T-DNA collections allow for an easy and straightforward identification of the tagged gene, serving as the basis of both forward and reverse genetic strategies. This study reports on the phenotypic and molecular characterization of an enhancer trap T-DNA collection in tomato (Solanum lycopersicum L.), which has been produced by Agrobacterium-mediated transformation using a binary vector bearing a minimal promoter fused to the uidA reporter gene. Two genes have been isolated from different T-DNA mutants, one of these genes codes for a UTP-glucose-1-phosphate uridylyltransferase involved in programmed cell death and leaf development, which means a novel gene function reported in tomato. Together, our results support that enhancer trapping is a powerful tool to identify novel genes and regulatory elements in tomato and that this T-DNA mutant collection represents a highly valuable resource for functional analyses in this fleshy-fruited model species. | es_ES |
dc.description.sponsorship | This work was supported by research grants from the Spanish Ministerio de Economia y Competitividad (AGL2012-40150-C02-01, AGL2012-40150-C02-02, AGL2015-64991-C3-1-R and AGL2015-64991-C3-3-R), Junta de Andalucia (P10-AGR-6931) and UE-FEDER. B.P. received a JAE-Doc research contract from the CSIC (Spain). PhD fellowships were funded by the FPU (M.G-A. and R.F.) and the FPI (M.P.A., S.S.A. F-L., A.O-A and L.C.) Programmes of the Ministerio de Ciencia e Innovacion, the JAE predoc Programme of the Spanish CSIC (G.G.), the CONACYT and Universidad de Sinaloa of Mexico (J.S.) and the LASPAU (J.L.Q.). The authors thank research facilities provided by the Campus de Excelencia Internacional Agroalimentario (CeiA3) | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Blackwell Publishing | es_ES |
dc.relation.ispartof | Plant Biotechnology Journal | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | T-DNA | es_ES |
dc.subject | Enhancer trapping | es_ES |
dc.subject | Solanum lycopersicum | es_ES |
dc.subject | Functional genomics | es_ES |
dc.subject | Insertional mutagenesis | es_ES |
dc.subject | GUS expression | es_ES |
dc.subject.classification | GENETICA | es_ES |
dc.title | A collection of enhancer trap insertional mutants for functional genomics in tomato | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1111/pbi.12728 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/Junta de Andalucía//P10-AGR-6931/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//AGL2012-40150-C03-01/ES/IDENTIFICACION, ETIQUETADO Y ANALISIS FUNCIONAL DE GENES IMPLICADOS EN EL CUAJADO DEL FRUTO DE TOMATE Y TOLERANCIA A LA SALINIDAD EN ESPECIES SILVESTRES RELACIONADAS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//AGL2015-64991-C3-3-R/ES/GENOMICA FUNCIONAL Y MEJORA GENETICA DE TOMATE: IMPORTANCIA AGRONOMICA DEL BALANCE DESARROLLO - ESTRES ABIOTICO/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.description.bibliographicCitation | Pérez-Martín, F.; Yuste-Lisbona, FJ.; Pineda Chaza, BJ.; Angarita-Díaz, MP.; García Sogo, B.; Antón Martínez, MT.; Sanchez Martín-Sauceda, S.... (2017). A collection of enhancer trap insertional mutants for functional genomics in tomato. Plant Biotechnology Journal. 15(11):1439-1452. https://doi.org/10.1111/pbi.12728 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1111/pbi.12728 | es_ES |
dc.description.upvformatpinicio | 1439 | es_ES |
dc.description.upvformatpfin | 1452 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 15 | es_ES |
dc.description.issue | 11 | es_ES |
dc.relation.pasarela | S\351693 | es_ES |
dc.contributor.funder | Junta de Andalucía | es_ES |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas | es_ES |
dc.contributor.funder | Consejo Nacional de Ciencia y Tecnología, México | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad | es_ES |