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The Tomato SlVIPP1 Gene Is Required for Plant Survival Through the Proper Development of Chloroplast Thylakoid Membrane

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The Tomato SlVIPP1 Gene Is Required for Plant Survival Through the Proper Development of Chloroplast Thylakoid Membrane

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dc.contributor.author Micol-Ponce, Rosa es_ES
dc.contributor.author García-Alcázar, Manuel es_ES
dc.contributor.author Capel, Carmen es_ES
dc.contributor.author Yuste-Lisbona, Fernando Juan es_ES
dc.contributor.author Pineda Chaza, Benito José es_ES
dc.contributor.author Atarés Huerta, Alejandro es_ES
dc.contributor.author García Sogo, Begoña es_ES
dc.contributor.author Capel, Juan es_ES
dc.contributor.author Moreno Ferrero, Vicente es_ES
dc.contributor.author Lozano, Rafael es_ES
dc.date.accessioned 2021-05-08T03:31:21Z
dc.date.available 2021-05-08T03:31:21Z
dc.date.issued 2020-08-26 es_ES
dc.identifier.uri http://hdl.handle.net/10251/166079
dc.description.abstract [EN] Since membranes play essential roles in all living beings, all cells have developed mechanisms for efficient and fast repair of membrane damage. InEscherichia coli, the Phage shock stress A (PspA) protein is involved in the maintenance of the integrity of its inner membrane in response to the damage produced by exposure to stress conditions. A role in thylakoid membrane maintenance and reorganization has been proposed for Vesicle Inducing Protein in Plastid 1 (VIPP1), the putative PspA ortholog inArabidopsis thaliana. While some membranes of plant cells have been extensively studied, the biosynthesis and maintenance of chloroplast thylakoid membrane remains poorly known. Here, we report the cloning and functional characterization of the tomato (Solanum lycopersicumL.) ortholog ofEscherichia coli PspAandArabidopsis thaliana VIPP1, which we dubbedSlVIPP1. Our genetic and molecular characterization ofslvipp1, an insertional mutant, allowed us to conclude that the tomatoSlVIPP1gene is needed for development, as ArabidopsisVIPP1, but notEscherichia coli PspA. Homozygousslvipp1tomato plants are albino and exhibit early lethality and highly aberrant chloroplast development with almost complete absence of thylakoids. The phenotype of tomato RNAi lines and that of additionalslvipp1alleles generated by CRISPR/Cas9 gene editing technology confirmed that the morphological and histological aberrations shown byslvipp1homozygotes are caused byVIPP1lack of function. We also found that tomatoSlVIPP1overexpression does not cause any visible effect on plant morphology and viability. Our work withslvipp1plants evidences thatSlVIPP1is an essential gene required for tomato survival, since its function is crucial for the proper formation and/or maintenance of thylakoid membranes. es_ES
dc.description.sponsorship This work was supported by research grants from the Spanish Ministry of Science and Innovation and the UE-European Regional Development Fund (grants PID2019-110833RB-C31 and PID2019-110833RB-C32) and the Research and Innovation Programme of the European Union Horizon 2020 (BRESOV Project, ID 774244). A PhD fellowship to MG-A was funded by the FPU Programme of the Spanish Ministry of Science and Innovation. The authors thank research facilities provided by the Campus de Excelencia Internacional Agroalimentario (CeiA3). es_ES
dc.language Inglés es_ES
dc.publisher Frontiers Media SA es_ES
dc.relation.ispartof Frontiers in Plant Science es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject SlVIPP1 es_ES
dc.subject Tomato es_ES
dc.subject Chloroplast es_ES
dc.subject Thylakoid membrane es_ES
dc.subject PspA es_ES
dc.subject Albinism es_ES
dc.subject Lethality es_ES
dc.subject.classification GENETICA es_ES
dc.title The Tomato SlVIPP1 Gene Is Required for Plant Survival Through the Proper Development of Chloroplast Thylakoid Membrane es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3389/fpls.2020.01305 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/774244/EU/Breeding for Resilient, Efficient and Sustainable Organic Vegetable production/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-110833RB-C31/ES/REGULACION GENETICA DE LA ACTIVIDAD DE LOS MERISTEMOS REPRODUCTIVOS Y SU PAPEL EN LA MEJORA DE LA PRODUCTIVIDAD DE TOMATE/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-110833RB-C32/ES/EDICION DE PROMOTORES DE GENES QUE REGULAN EL DESARROLLO REPRODUCTIVO EN TOMATE COMO ESTRATEGIA PARA MANTENER LA PRODUCCION EN CONDICIONES DE ESTRES ABIOTICO/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Micol-Ponce, R.; García-Alcázar, M.; Capel, C.; Yuste-Lisbona, FJ.; Pineda Chaza, BJ.; Atarés Huerta, A.; García Sogo, B.... (2020). The Tomato SlVIPP1 Gene Is Required for Plant Survival Through the Proper Development of Chloroplast Thylakoid Membrane. Frontiers in Plant Science. 11:1-14. https://doi.org/10.3389/fpls.2020.01305 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3389/fpls.2020.01305 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 14 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 11 es_ES
dc.identifier.eissn 1664-462X es_ES
dc.identifier.pmid 32983195 es_ES
dc.identifier.pmcid PMC7479267 es_ES
dc.relation.pasarela S\423097 es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
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