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Zinc Chloride: Time-Dependent Cytotoxicity, Proliferation and Promotion of Glycoprotein Synthesis and Antioxidant Gene Expression in Human Keratinocytes

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Zinc Chloride: Time-Dependent Cytotoxicity, Proliferation and Promotion of Glycoprotein Synthesis and Antioxidant Gene Expression in Human Keratinocytes

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dc.contributor.author Salesa, Beatriz es_ES
dc.contributor.author Sabater i Serra, Roser es_ES
dc.contributor.author Serrano-Aroca, Ángel es_ES
dc.date.accessioned 2022-04-05T06:55:05Z
dc.date.available 2022-04-05T06:55:05Z
dc.date.issued 2021-11 es_ES
dc.identifier.uri http://hdl.handle.net/10251/181796
dc.description.abstract [EN] Zinc ions are involved in the biology of cell growth, proliferation, differentiation or apoptosis by regulating many biological molecules, such as transcription factors, enzymes and growth factors. In this study, the time-dependent cytotoxicity, cell proliferation and gene expression in human keratinocytes HaCaT cells were evaluated when exposed to ZnCl2. The results of this study showed non-cytotoxic effects up to 10 mu g/mL after 24 h, no significant effect on cell proliferation when exposed to 5 or 1 mu g/mL ZnCl2 at 72 h and upregulation of eight genes, with great potential in the biomedical field, particularly for regenerative-medicine applications and wound healing. The use of ionic metals such as zinc (Zn2+) is providing promising results in regenerative medicine. In this study, human keratinocytes (HaCaT cells) were treated with different concentrations of zinc chloride (ZnCl2), ranging from 1 to 800 mu g/mL, for 3, 12 and 24 h. The results showed a time-concentration dependence with three non-cytotoxic concentrations (10, 5 and 1 mu g/mL) and a median effective concentration value of 13.5 mu g/mL at a cell exposure to ZnCl2 of 24 h. However, the zinc treatment with 5 or 1 mu g/mL had no effect on cell proliferation in HaCaT cells in relation to the control sample at 72 h. The effects of the Zn2+ treatment on the expression of several genes related to glycoprotein synthesis, oxidative stress, proliferation and differentiation were assessed at the two lowest non-cytotoxic concentrations after 24 h of treatment. Out of 13 analyzed genes (superoxide dismutase 1 (SOD1), catalase (CAT), matrix metallopeptidase 1 (MMP1), transforming growth factor beta 1 (TGFB1), glutathione peroxidase 1 (GPX1), fibronectin 1 (FN1), hyaluronan synthase 2 (HAS2), laminin subunit beta 1 (LAMB1), lumican (LUM), cadherin 1 (CDH1), collagen type IV alpha (COL4A1), fibrillin (FBN) and versican (VCAN)), Zn2+ was able to upregulate SOD1, CAT, TGFB1, GPX1, LUM, CDH1, FBN and VCAN, with relative expression levels of at least 1.9-fold with respect to controls. We found that ZnCl2 promoted glycoprotein synthesis and antioxidant gene expression, thus confirming its great potential in biomedicine. es_ES
dc.description.sponsorship This study was founded by the Fundacion Universidad Catolica de Valencia San Vicente Martir, Grant 2020-231-006UCV, the Spanish Ministry of Science and Innovation (PID2020-119333RB-I00/AEI/10.13039/501100011033) (awarded to A.S.-A.) and the FEDER/Spanish Ministry of Science and Innovation-Agencia Estatal de Investigacion) through the Project RTI2018-097862-B-C21 (awarded to R.S.i.S). CIBER-BBN is an initiative funded by the VI National R&D&I Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program. CIBER Actions are financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. es_ES
dc.language Inglés es_ES
dc.publisher MDPI AG es_ES
dc.relation.ispartof Biology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Biometals es_ES
dc.subject Zinc ions es_ES
dc.subject Human keratinocytes es_ES
dc.subject Cytotoxicity es_ES
dc.subject Gene expression es_ES
dc.subject Biomedical applications es_ES
dc.subject.classification INGENIERIA ELECTRICA es_ES
dc.title Zinc Chloride: Time-Dependent Cytotoxicity, Proliferation and Promotion of Glycoprotein Synthesis and Antioxidant Gene Expression in Human Keratinocytes es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/biology10111072 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-119333RB-I00/ES/SOPORTES BIOFUNCIONALES CON CAPACIDAD OSTEOINDUCTORA Y ANTIMICROBIANA PARA INGENIERIA TISULAR OSEA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UCV//2020-231-006UCV/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-097862-B-C21/ES/MICROENTORNOS BIOACTIVOS, ELECTROCONDUCTIVOS Y ANTIMICROBIANOS CON CAPACIDAD DE ESTIMULAR LA REGENERACION OSEA Y PREVENIR INFECCIONES MULTIRRESISTENTES/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ISCIII//CIBER-BBN/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería Eléctrica - Departament d'Enginyeria Elèctrica es_ES
dc.description.bibliographicCitation Salesa, B.; Sabater I Serra, R.; Serrano-Aroca, Á. (2021). Zinc Chloride: Time-Dependent Cytotoxicity, Proliferation and Promotion of Glycoprotein Synthesis and Antioxidant Gene Expression in Human Keratinocytes. Biology. 10(11):1-13. https://doi.org/10.3390/biology10111072 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/biology10111072 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 13 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 10 es_ES
dc.description.issue 11 es_ES
dc.identifier.eissn 2079-7737 es_ES
dc.identifier.pmid 34827065 es_ES
dc.identifier.pmcid PMC8615178 es_ES
dc.relation.pasarela S\452227 es_ES
dc.contributor.funder Instituto de Salud Carlos III es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Universidad Católica de Valencia San Vicente Mártir es_ES
upv.costeAPC 1767,55 es_ES


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