Zorraquino Lozano, MA.; Althaus, RL.; Roca Marugán, MI.; Molina Pons, MP. (2011). Heat Treatment Effects on the Antimicrobial Activity of Macrolide and Lincosamide Antibiotics in Milk. Journal of Food Protection. 74(2):311-315. https://doi.org/10.4315/0362-028X.JFP-10-297
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/28390
Title:
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Heat Treatment Effects on the Antimicrobial Activity of Macrolide and Lincosamide Antibiotics in Milk
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Author:
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Zorraquino Lozano, Miguel Angel
Althaus, R. L.
Roca Marugán, Marta Isabel
Molina Pons, Mª Pilar
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UPV Unit:
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Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal
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Issued date:
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Abstract:
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[EN] Aims: In this study, a microbiological method of dichotomous response using Bacillus cereus was designed and optimized to detect tetracyclines (TCs) at concentrations near to the maximum residue limits (MRLs). Methods ...[+]
[EN] Aims: In this study, a microbiological method of dichotomous response using Bacillus cereus was designed and optimized to detect tetracyclines (TCs) at concentrations near to the maximum residue limits (MRLs). Methods and Results: In a first stage, the response time of bioassay was reduced to 5h when the logarithm of spore concentration (log S) was increased. Later, a Plackett Burman design (2 6-3) was analysed using logistic regression model. This design indicates significant effects of log S and chloramphenicol (CAP) on the detection limit (DL) of TC. Then, the response surfaces (RS) of the TCs DTs as a function of log S and CAP were plotted using a Dohlert design and the logistic regression model. These RS show a linear decrease with the raise of CAP and a quadratic effect of log S. Finally, the DTs of TC (109¿gl -1) and oxytetracycline (100¿gl -1) were adjusted to their MRLs through the desirability function. Conclusions: By successive application of experimental design techniques could be optimized a bioassay for the detection of TC residues in milk. The best conditions have been achieved when the assay was made with log S=5·12 and CAP=470¿gl -1. Significance and Impact of the Study: Experimental design techniques together with the logistic regression model and the desirability function represent an adequate tool for the optimization of a bioassay with binary response. © 2011 The Authors. Letters in Applied Microbiology © 2011 The Society for Applied Microbiology.
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Subjects:
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Bioassay
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Experimental design
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Milk
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Screening test
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Tetracycline
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Chloramphenicol
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Bacterium
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Logistics
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Optimization
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Regression analysis
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Article
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Bacillus cereus
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Bacterial spore
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Chemometric analysis
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Limit of detection
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Logistic regression analysis
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Nonhuman
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Animals
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Anti-Bacterial Agents
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Biological Assay
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Food Analysis
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Logistic Models
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Microbial Sensitivity Tests
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Sensitivity and Specificity
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Spores, Bacterial
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Tetracyclines
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Copyrigths:
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Cerrado |
Source:
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Journal of Food Protection. (issn:
0362-028X
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DOI:
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10.4315/0362-028X.JFP-10-297
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Publisher:
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International Association for Food Protection
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Publisher version:
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http://doi.org/10.4315/0362-028X.JFP-10-297
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Project ID:
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info:eu-repo/grantAgreement/MICYT//AGL2003-03663/ES/EFECTO DE LOS TRATAMIENTOS TERMICOS SOBRE LA ACTIVIDAD Y%2FO PRESENCIA DE SUSTANCIAS ANTIMICROBIANAS EN LA LECHE/
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Thanks:
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This research has been carried out as part of the AGL2003-03663 project and has been financially assisted by the Spanish Ministry of Education and Science (Madrid, Spain). The authors thank the Polytechnic University of ...[+]
This research has been carried out as part of the AGL2003-03663 project and has been financially assisted by the Spanish Ministry of Education and Science (Madrid, Spain). The authors thank the Polytechnic University of Valencia (UPV, Spain) for funding Dr. Rafael Althaus's collaboration with the Institute for Animal Science and Technology (UPV).
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Type:
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Artículo
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