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Carbon nanoparticles in lateral flow methods to detect genes encoding virulence factors of Shiga toxin-producing Escherichia coli

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Carbon nanoparticles in lateral flow methods to detect genes encoding virulence factors of Shiga toxin-producing Escherichia coli

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dc.contributor.author Noguera Murray, Patricia Silvestre es_ES
dc.contributor.author Posthuma-Trumpie, G.A. es_ES
dc.contributor.author van Tuil, M. es_ES
dc.contributor.author van der Wal, F.J. es_ES
dc.contributor.author de Boer, A. es_ES
dc.contributor.author Moers, A.P.H.A. es_ES
dc.contributor.author van Amerongen, A. es_ES
dc.date.accessioned 2013-07-08T14:05:19Z
dc.date.issued 2011
dc.identifier.issn 1618-2642
dc.identifier.uri http://hdl.handle.net/10251/30834
dc.description.abstract The use of carbon nanoparticles is shown for the detection and identification of different Shiga toxin-producing Escherichia coli virulence factors (vt1, vt2, eae and ehxA) and a 16S control (specific for E. coli) based on the use of lateral flow strips (nucleic acid lateral flow immunoassay, NALFIA). Prior to the detection with NALFIA, a rapid amplification method with tagged primers was applied. In the evaluation of the optimised NALFIA strips, no cross-reactivity was found for any of the antibodies used. The limit of detection was higher than for quantitative PCR (q-PCR), in most cases between 10 4 and 10 5 colony forming units/mL or 0.1-0.9 ng/¿L DNA. NALFIA strips were applied to 48 isolates from cattle faeces, and results were compared to those achieved by q-PCR. E. coli virulence factors identified by NALFIA were in very good agreement with those observed in q-PCR, showing in most cases sensitivity and specificity values of 1.0 and an almost perfect agreement between both methods (kappa coefficient larger than 0.9). The results demonstrate that the screening method developed is reliable, cost-effective and user-friendly, and that the procedure is fast as the total time required is <1 h, which includes amplification. © 2010 The Author(s). es_ES
dc.description.sponsorship This work was partially supported by the Generalitat Valenciana (BEST/2009/026), the Universidad Politecnica de Valencia (PAID-00-09-2837), and by the Dutch Ministry of Agriculture, Nature and Food Quality (KennisBasis 6 programme). The authors would like to thank Dr. Eva Moller Nielsen at the Danish Veterinary Institute (Copenhagen, Denmark) for providing E. coli control strains and Dr. Lutz Geue (Friedrich-Loeffler-Institut, Wusterhausen, Germany) and Dr. Dorte Dopfer (School of Veterinary Medicine, University of Wisconsin, Madison, WI, USA) for field isolates. en_EN
dc.language Inglés es_ES
dc.publisher Springer Verlag es_ES
dc.relation Generalitat Valenciana [BEST/2009/026] es_ES
dc.relation Universidad Politecnica de Valencia [PAID-00-09-2837] es_ES
dc.relation Dutch Ministry of Agriculture, Nature and Food Quality es_ES
dc.relation.ispartof Analytical and Bioanalytical Chemistry es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Carbon nanoparticles es_ES
dc.subject E. coli es_ES
dc.subject Nucleic acid lateral flow immunoassay es_ES
dc.subject Screening method es_ES
dc.subject Shiga toxin es_ES
dc.subject Verotoxin es_ES
dc.subject Carbon nano particles es_ES
dc.subject Biomolecules es_ES
dc.subject Escherichia coli es_ES
dc.subject Immunology es_ES
dc.subject Nanoparticles es_ES
dc.subject Nucleic acids es_ES
dc.subject Toxic materials es_ES
dc.subject Polymerase chain reaction es_ES
dc.subject Bacterial DNA es_ES
dc.subject Carbon es_ES
dc.subject Nanoparticle es_ES
dc.subject Virulence factor es_ES
dc.subject Animal es_ES
dc.subject Article es_ES
dc.subject Bacterial gene es_ES
dc.subject Cattle es_ES
dc.subject Chemistry es_ES
dc.subject Economics es_ES
dc.subject Genetics es_ES
dc.subject Immunoassay es_ES
dc.subject Instrumentation es_ES
dc.subject Isolation and purification es_ES
dc.subject Methodology es_ES
dc.subject Sensitivity and specificity es_ES
dc.subject Shiga toxin producing Escherichia coli es_ES
dc.subject Animals es_ES
dc.subject DNA, Bacterial es_ES
dc.subject Genes, Bacterial es_ES
dc.subject Shiga-Toxigenic Escherichia coli es_ES
dc.subject Virulence Factors es_ES
dc.subject Bos es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Carbon nanoparticles in lateral flow methods to detect genes encoding virulence factors of Shiga toxin-producing Escherichia coli es_ES
dc.type Artículo es_ES
dc.embargo.lift 10000-01-01
dc.embargo.terms forever es_ES
dc.identifier.doi 10.1007/s00216-010-4334-z
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic es_ES
dc.description.bibliographicCitation Noguera Murray, PS.; Posthuma-Trumpie, G.; Van Tuil, M.; Van Der Wal, F.; De Boer, A.; Moers, A.; Van Amerongen, A. (2011). Carbon nanoparticles in lateral flow methods to detect genes encoding virulence factors of Shiga toxin-producing Escherichia coli. Analytical and Bioanalytical Chemistry. 399:831-838. doi:10.1007/s00216-010-4334-z es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.1007/s00216-010-4334-z es_ES
dc.description.upvformatpinicio 831 es_ES
dc.description.upvformatpfin 838 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 399 es_ES
dc.relation.senia 211030
dc.identifier.eissn 1618-2650
dc.identifier.pmid 21046083 en_EN
dc.identifier.pmcid PMC3015178 en_EN
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