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dc.contributor.author | Brun Sánchez, Eva María | es_ES |
dc.contributor.author | Puchades Pla, Rosa | es_ES |
dc.contributor.author | Maquieira Catala, Ángel | es_ES |
dc.date.accessioned | 2013-11-21T08:34:48Z | |
dc.date.issued | 2013-03-15 | |
dc.identifier.issn | 0003-2700 | |
dc.identifier.uri | http://hdl.handle.net/10251/33864 | |
dc.description.abstract | Compact disc (CD) surface modification with different reflective materials offers new working perspectives as bioanalytical platforms. The potential of gold, carbon, and aluminum low-reflectivity compact discs as a substrate for microassaying is presented. CD polycarbonate bases were coated with these reflecting materials, maintaining optical properties. Probes immobilization onto reflective layers was studied by both passive adsorption and covalent linking. Different chemical modifications were made on substrates to provide functional groups capable of anchoring probes with primary amines covalently. Thus, self-assembled monolayers were performed by chemisorption of 11-mercaptoundecanoic acid on gold surfaces, and silanization with N- (trimethoxysilylpropyl)ethylenediamine triacetic acid on aluminum to provide a carboxylic acid functional group. Carbon oxidation with oxygen plasma afforded similar functionalization on the discs coated with this material. Performance of the studied materials as reflective layers was evaluated, and as proof of concept, a microimmunoassay for a neurotoxic compound was studied on the three surfaces. The results show the possibility of doing assays on this new supports with good analytical performances while maintaining discs¿ optical and mechanical properties to be read by a CD player. | es_ES |
dc.description.sponsorship | The authors are thankful to Javier Carrascosa for his valuable aid in CD reading, to Juan Soto for obtaining cyclic voltammograms, and to the Servicio de Microscopia electronica (Universitat Politecnica de Valencia) for their kind assistance in sputtering depositions and AFM. This work has been supported by Projects FEDER MICINN CTQ2010-15943, GVA PROM-ETEO 2010/008 and ACOMP/2012/158, and the program PAID-05-12 (Universitat Politecnica de Valencia). | en_EN |
dc.format.extent | 9 | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | American Chemical Society | es_ES |
dc.relation.ispartof | Analytical Chemistry | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Electron Microscopy Service of the UPV | es_ES |
dc.subject.classification | QUIMICA ANALITICA | es_ES |
dc.title | Gold, Carbon and Aluminum Low-reflectivity Compact Discs as Microassaying Platforms | es_ES |
dc.type | Artículo | es_ES |
dc.embargo.lift | 10000-01-01 | |
dc.embargo.terms | forever | es_ES |
dc.identifier.doi | 10.1021/ac4004985 | |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//CTQ2010-15943/ES/ESTUDIO DE NUEVAS VIAS DE DESARROLLO DE BIOMEMS PARA SCREENING MASIVO. DEMOSTRACION DE CONCEPTO COMO HERRAMIENTA DE ANALISIS APLICABLE EN "OMICAS"./ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/UPV//PAID-05-12/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/Generalitat Valenciana//PROMETEO%2F2010%2F008/ES/PROMETEO%2F2010%2F008/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/Generalitat Valenciana//ACOMP%2F2012%2F158/ES/ACOMP%2F2012%2F158/ | es_ES |
dc.rights.accessRights | Cerrado | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Química - Departament de Química | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic | es_ES |
dc.description.bibliographicCitation | Brun Sánchez, EM.; Puchades Pla, R.; Maquieira Catala, Á. (2013). Gold, Carbon and Aluminum Low-reflectivity Compact Discs as Microassaying Platforms. Analytical Chemistry. 4178-4186. https://doi.org/10.1021/ac4004985 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1021/ac4004985 | es_ES |
dc.description.upvformatpinicio | 4178 | es_ES |
dc.description.upvformatpfin | 4186 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.relation.senia | 236487 | |
dc.contributor.funder | Universitat Politècnica de València | es_ES |