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Role of superhydrophobicity in the biological activity of fibronectin at the cell¿material interface

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Role of superhydrophobicity in the biological activity of fibronectin at the cell¿material interface

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dc.contributor.author Ballester Beltrán, José es_ES
dc.contributor.author Rico Tortosa, Patricia María es_ES
dc.contributor.author Moratal Pérez, David es_ES
dc.contributor.author Song, Wenlong es_ES
dc.contributor.author Mano, Joao F es_ES
dc.contributor.author Salmerón Sánchez, Manuel es_ES
dc.date.accessioned 2015-07-24T10:42:03Z
dc.date.available 2015-07-24T10:42:03Z
dc.date.issued 2011
dc.identifier.issn 1744-683X
dc.identifier.uri http://hdl.handle.net/10251/53707
dc.description.abstract Protein adsorption and cellular behavior depend strongly on the wettability of substrates. Such studies are scarce for surfaces exhibiting extreme values of contact angles. Fibronectin (FN) adsorption and adhesion of MC3T3-E1 cells were investigated on superhydrophobic polystyrene (SH-PS) surfaces and compared with the corresponding smooth polystyrene (PS) substrate and the control glass. The FN surface density was lower on the SH-PS than on PS, and the adsorbed protein showed altered conformation of cell adhesion domains, as obtained by ELISA with monoclonal antibodies. Cell adhesion occurred on the SH-PS without the formation of mature focal adhesions, as assessed by immunofluorescence for vinculin, talin and paxillin. Correspondingly, the development of the actin cytoskeleton was delayed and without the presence of defined F-actin fibers. FAK phosphorylation was reduced on SH-PS, as compared with PS and the control glass. Also, cell contractility was diminished on the SH-PS as revealed by phosphorylation of myosin light chain (pMLC). Likewise, FN reorganization and secretion were impaired on the superhydrophobic surfaces. Cell proliferation was significantly lower in SH-PS as compared with PS up to 21 days of culture. © 2011 The Royal Society of Chemistry. es_ES
dc.description.sponsorship The support of the Spanish Ministry of Science and Innovation through project MAT2009-14440-C02-01 is acknowledged. CIBER-BBN is an initiative funded by the VI National R&D&i Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions and financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. This work was supported by funds for research in the field of Regenerative Medicine through the collaboration agreement from the Conselleria de Sanidad (Generalitat Valenciana), and the Instituto de Salud Carlos III. en_EN
dc.language Inglés es_ES
dc.publisher Royal Society of Chemistry es_ES
dc.relation.ispartof Soft Matter es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Actin cytoskeleton es_ES
dc.subject Cellular behaviors es_ES
dc.subject Extreme value es_ES
dc.subject F-actin fibers es_ES
dc.subject Fibronectin adsorption es_ES
dc.subject Focal adhesions es_ES
dc.subject MC3T3-E1 cell es_ES
dc.subject Myosin light chains es_ES
dc.subject Paxillin es_ES
dc.subject Protein adsorption es_ES
dc.subject Super-hydrophobic surfaces es_ES
dc.subject Superhydrophobic es_ES
dc.subject Superhydrophobicity es_ES
dc.subject Surface density es_ES
dc.subject Vinculin es_ES
dc.subject Adhesion es_ES
dc.subject Adsorption es_ES
dc.subject Cell adhesion es_ES
dc.subject Cell proliferation es_ES
dc.subject Cells es_ES
dc.subject Contact angle es_ES
dc.subject Glass es_ES
dc.subject Hydrophobicity es_ES
dc.subject Monoclonal antibodies es_ES
dc.subject Phosphorylation es_ES
dc.subject Polystyrenes es_ES
dc.subject Proteins es_ES
dc.subject Substrates es_ES
dc.subject Surfaces es_ES
dc.subject Cell culture es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.subject.classification TECNOLOGIA ELECTRONICA es_ES
dc.subject.classification TERMODINAMICA APLICADA (UPV) es_ES
dc.title Role of superhydrophobicity in the biological activity of fibronectin at the cell¿material interface es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1039/c1sm06102j
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//MAT2009-14440-C02-01/ES/Dinamica De Las Proteinas De La Matriz En La Interfase Celula-Material/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería Electrónica - Departament d'Enginyeria Electrònica es_ES
dc.description.bibliographicCitation Ballester Beltrán, J.; Rico Tortosa, PM.; Moratal Pérez, D.; Song, W.; Mano, JF.; Salmerón Sánchez, M. (2011). Role of superhydrophobicity in the biological activity of fibronectin at the cell¿material interface. Soft Matter. 7(22):10803-10811. https://doi.org/10.1039/c1sm06102j es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/ 10.1039/c1sm06102j es_ES
dc.description.upvformatpinicio 10803 es_ES
dc.description.upvformatpfin 10811 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 7 es_ES
dc.description.issue 22 es_ES
dc.relation.senia 209135
dc.identifier.eissn 1744-6848
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Conselleria de Sanitat Universal i Salut Pública de la Generalitat Valenciana es_ES
dc.contributor.funder Instituto de Salud Carlos III es_ES
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