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dc.contributor.author | Hillung, Julia | es_ES |
dc.contributor.author | Elena Fito, Santiago Fco | es_ES |
dc.contributor.author | Cuevas Torrijos, Jose Manuel | es_ES |
dc.date.accessioned | 2016-05-12T10:56:04Z | |
dc.date.available | 2016-05-12T10:56:04Z | |
dc.date.issued | 2013-11-13 | |
dc.identifier.issn | 1471-2148 | |
dc.identifier.uri | http://hdl.handle.net/10251/63967 | |
dc.description.abstract | Background: Pipo was recently described as a new ORF encoded within the genome of the Potyviridae family members (PNAS 105: 5897-5902, 2008). It is embedded within the P3 cistron and is translated in the +2 reading frame relative to the potyviral long ORF as the P3N-PIPO fusion protein. In this work, we first collected pipo nucleotide sequences available for different isolates of 48 Potyvirus species. Second, to determine the biological implications of variation in pipo length, we measured infectivity, viral accumulation, cell-to-cell and systemic movements for two Turnip mosaic virus (TuMV) variants with pipo alleles of different length in three different susceptible host species, and tested for differences between the two variants. Results: In addition to inter-specific variation, there was high variation in the length of the PIPO protein among isolates within species (ranging from 1 to 89 amino acids). Furthermore, selection analyses on the P3 cistron did not account for the existence of stop codons in the pipo ORF, but showed that positive selection was significant in the overlapping region for Potato virus Y (PVY) and TuMV. In some cases, variability in length was associated with host species, geographic provenance and/or other strain features. We found significant empirical differences among the phenotypes associated with TuMV pipo alleles, though the magnitude and sign of the effects were host-dependent. Conclusions: The combination of computational molecular evolution analyses and experiments stemming from these analyses provide clues about the selective pressures acting upon the different-length pipo alleles and show that variation in length may be maintained by host-driven selection. | es_ES |
dc.description.sponsorship | We thank Jose A. Daros for providing the TuMV-GFP infectious clone, Mario A. Fares and Guillaume Lafforgue for fruitful discussion, Guillermo Rodrigo for assistance with MATLAB and for critically reading of the manuscript, Mark P. Zwart for critically reading the manuscript, and Francisca de la Iglesia for excellent technical assistance. Funding for this work was provided by the Spanish Direccion General de Investigacion Cientifica y Tecnica grant BFU2012-30805 (to SFE), a predoctoral fellowship from the Spanish Ministerio de Economia y Competitividad (to JH), and a postdoctoral contract from CSIC JAE-doc program (to JMC). | en_EN |
dc.language | Inglés | es_ES |
dc.publisher | BioMed Central | es_ES |
dc.relation.ispartof | BMC Evolutionary Biology | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | Bayesian phylogenetic methods | es_ES |
dc.subject | Host-range determinants | es_ES |
dc.subject | Molecular evolution | es_ES |
dc.subject | Potyvirus | es_ES |
dc.subject | Virus evolution | es_ES |
dc.subject | Virus fitness components | es_ES |
dc.title | Intra-specific variability and biological relevance of P3N-PIPO protein length in potyviruses | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1186/1471-2148-13-249 | |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//BFU2012-30805/ES/EVOLUTIONARY SYSTEMS VIROLOGY: EPISTASIS AND THE RUGGEDNESS OF ADAPTIVE LANDSCAPES, MUTATIONS IN REGULATORY SEQUENCES, AND THE HOST DETERMINANTS OF VIRAL FITNESS/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.description.bibliographicCitation | Hillung, J.; Elena Fito, SF.; Cuevas Torrijos, JM. (2013). Intra-specific variability and biological relevance of P3N-PIPO protein length in potyviruses. BMC Evolutionary Biology. 13(249):1-10. https://doi.org/10.1186/1471-2148-13-249 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1186/1471-2148-13-249 | es_ES |
dc.description.upvformatpinicio | 1 | es_ES |
dc.description.upvformatpfin | 10 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 13 | es_ES |
dc.description.issue | 249 | es_ES |
dc.relation.senia | 260570 | es_ES |
dc.identifier.pmid | 24225158 | en_EN |
dc.identifier.pmcid | PMC3840659 | en_EN |
dc.contributor.funder | Ministerio de Economía y Competitividad | es_ES |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas | es_ES |
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