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Specific Argonautes Selectively Bind Small RNAs Derived from Potato Spindle Tuber Viroid and Attenuate Viroid Accumulation In Vivo

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Specific Argonautes Selectively Bind Small RNAs Derived from Potato Spindle Tuber Viroid and Attenuate Viroid Accumulation In Vivo

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dc.contributor.author Minoia, Sofia es_ES
dc.contributor.author Carbonell, Alberto es_ES
dc.contributor.author Di Serio, Francesco es_ES
dc.contributor.author Gisel, Andreas es_ES
dc.contributor.author Carrington, James C. es_ES
dc.contributor.author Navarro, Beatriz es_ES
dc.contributor.author Flores Pedauye, Ricardo es_ES
dc.date.accessioned 2017-01-23T12:09:42Z
dc.date.available 2017-01-23T12:09:42Z
dc.date.issued 2014-10
dc.identifier.issn 0022-538X
dc.identifier.uri http://hdl.handle.net/10251/77220
dc.description.abstract The identification of viroid-derived small RNAs (vd-sRNAs) of 21 to 24 nucleotides (nt) in plants infected by viroids (infectious non-protein-coding RNAs of just 250 to 400 nt) supports their targeting by Dicer-like enzymes, the first host RNA-silencing barrier. However, whether viroids, like RNA viruses, are also targeted by the RNA-induced silencing complex (RISC) remains controversial. At the RISC core is one Argonaute (AGO) protein that, guided by endogenous or viral sRNAs, targets complementary RNAs. To examine whether AGO proteins also load vd-sRNAs, leaves of Nicotiana benthamiana infected by potato spindle tuber viroid (PSTVd) were agroinfiltrated with plasmids expressing epitope-tagged versions of AGO1, AGO2, AGO3, AGO4, AGO5, AGO6, AGO7, AGO9, and AGO10 from Arabidopsis thaliana. Immunoprecipitation analyses of the agroinfiltrated halos revealed that all AGOs except AGO6, AGO7, and AGO10 associated with vd-sRNAs: AGO1, AGO2, and AGO3 preferentially with those of 21 and 22 nt, while AGO4, AGO5, and AGO9 additionally bound those of 24 nt. Deep-sequencing analyses showed that sorting of vd-sRNAs into AGO1, AGO2, AGO4, and AGO5 depended essentially on their 5=-terminal nucleotides, with the pro- files of the corresponding AGO-loaded vd-sRNAs adopting specific hot spot distributions along the viroid genome. Furthermore, agroexpression of AGO1, AGO2, AGO4, and AGO5 on PSTVd-infected tissue attenuated the level of the genomic RNAs, suggesting that they, or their precursors, are RISC targeted. In contrast to RNA viruses, PSTVd infection of N. benthamiana did not affect miR168-mediated regulation of the endogenous AGO1, which loaded vd-sRNAs with specificity similar to that of its A. thaliana counterpart. es_ES
dc.description.sponsorship Research in the laboratory of R. F. is currently funded by grant BFU2011-28443 from the Ministerio de Economia y Competitividad (MINECO, Spain). S.M. has been supported by a fellowship and a pre-doctoral contract from MINECO. Research in the laboratory of B.N. and F.D.S. has been funded by a dedicated grant from the Ministero dell'Economia e Finanze Italiano to the CNR (CISIA; Legge no. 191/2009). Research in the laboratory of J.C.C. was supported by grants from the National Science Foundation (MCB-0956526 and MCB-1231726) and the National Institutes of Health (AI043288). en_EN
dc.language Inglés es_ES
dc.publisher American Society for Microbiology es_ES
dc.relation Ministerio de Economia y Competitividad (MINECO, Spain) BFU2011-28443 es_ES
dc.relation MINECO es_ES
dc.relation Ministero dell'Economia e Finanze Italiano (CISIA) 191/2009 es_ES
dc.relation National Science Foundation MCB-0956526 es_ES
dc.relation National Science Foundation MCB-1231726 es_ES
dc.relation National Institutes of Health AI043288 es_ES
dc.relation.ispartof Journal of Virology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.title Specific Argonautes Selectively Bind Small RNAs Derived from Potato Spindle Tuber Viroid and Attenuate Viroid Accumulation In Vivo es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1128/JVI.01404-14
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Minoia, S.; Carbonell, A.; Di Serio, F.; Gisel, A.; Carrington, JC.; Navarro, B.; Flores Pedauye, R. (2014). Specific Argonautes Selectively Bind Small RNAs Derived from Potato Spindle Tuber Viroid and Attenuate Viroid Accumulation In Vivo. Journal of Virology. 88(20):11933-11945. doi:10.1128/JVI.01404-14 es_ES
dc.description.accrualMethod Senia es_ES
dc.relation.publisherversion http://dx.doi.org/10.1128/JVI.01404-14 es_ES
dc.description.upvformatpinicio 11933 es_ES
dc.description.upvformatpfin 11945 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 88 es_ES
dc.description.issue 20 es_ES
dc.relation.senia 287955 es_ES
dc.identifier.pmid 25100851 en_EN
dc.identifier.pmcid PMC4178711 en_EN


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