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Role of calcium on the initiation of sperm motility in the European eel

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Role of calcium on the initiation of sperm motility in the European eel

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dc.contributor.author Pérez Igualada, Luz María es_ES
dc.contributor.author Vilchez Olivencia, Maria Carmen es_ES
dc.contributor.author Gallego Albiach, Victor es_ES
dc.contributor.author MORINI, MARINA es_ES
dc.contributor.author Peñaranda, D.S. es_ES
dc.contributor.author Asturiano Nemesio, Juan Francisco es_ES
dc.date.accessioned 2017-04-25T11:22:40Z
dc.date.available 2017-04-25T11:22:40Z
dc.date.issued 2016-01
dc.identifier.issn 1095-6433
dc.identifier.uri http://hdl.handle.net/10251/79931
dc.description.abstract [EN] Sperm from European eel males treated with hCG(rec), was washed in a calcium free extender, and sperm motility was activated both in the presence (seawater, SW) and in the absence of calcium (NaCI + EDTA), and treated with calcium inhibitors or modulators. The sperm motility parameters were evaluated by a computer-assisted sperm analysis (CASA) system, and changes in the [Ca2+](i) fluorescence (and in [Na+](i) in some cases) were evaluated by flow cytometry. After sperm motility was activated in a medium containing Ca2+ (seawater, SW) the intracellular fluorescence emitted by Ca2+ increased 4-6-fold compared to the levels in quiescent sperm. However, while sperm activation in a Ca-free media (NaCI + EDTA) resulted in a percentage of motility similar to seawater, the [Ca2+](i) levels did not increase at all. This result strongly suggests that increasing [Ca2+](i) is not a pre-requisite for the induction of sperm motility in European eel sperm. Several sperm velocities (VCL, VSL, VAP) decreased when sperm was activated in the Ca-free activator, thus supporting the theory that Ca2+ has a modulatory effect on sperm motility. The results indicate that a calcium/sodium exchanger (NCX) which is inhibited by bepridil and a calcium calmodulin kinase (inhibited by W-7), are involved in the sperm motility of the European eel. Our results indicate that the increase in [Ca2+](i) concentrations during sperm activation is due to an influx from the external medium, but, unlike in most other species, it does not appear to be necessary for the activation of motility in European eel sperm. (c) 2015 Elsevier Inc All rights reserved. es_ES
dc.description.sponsorship Funded from the SPERMOT project (Spanish Ministry of Science and Innovation, MICINN; AGL2010-16009). M.C. Vilchez has a predoctoral grant from UPV PAID Program (2011-S2-02-6521), Marina Morini has a predoctoral grant from Generalitat Valenciana (Programa Grisolia, GRISOLIA/2012/006), Victor Gallego has a postdoctoral contract from UPV (PAID-10-14), and David S. Penaranda was supported by MICINN (PTA2011-4948-I) and UPV (PTA2011-4948-I). Grants to attend meetings were received from COST Office (Food and Agriculture COST Action FA1205: AQUAGAMETE). en_EN
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject Anguilla anguilla es_ES
dc.subject Calcium/sodium exchanger es_ES
dc.subject Flow cytometry es_ES
dc.subject Intracellular ions es_ES
dc.subject Sperm activation es_ES
dc.subject Sperm physiology es_ES
dc.subject Sperm kinetics es_ES
dc.subject.classification BIOLOGIA ANIMAL es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Role of calcium on the initiation of sperm motility in the European eel es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.cbpa.2015.10.009
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//AGL2010-16009/ES/MECANISMOS FISIOLOGICOS IMPLICADOS EN LA ESPERMIACION Y EN LA ADQUISICION DE MOTILIDAD ESPERMATICA EN LA ANGUILA EUROPEA (ANGUILLA ANGUILLA)/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Pérez Igualada, LM.; Vilchez Olivencia, MC.; Gallego Albiach, V.; Morini, M.; Peñaranda, D.; Asturiano Nemesio, JF. (2016). Role of calcium on the initiation of sperm motility in the European eel. Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology. 191:98-106. https://doi.org/10.1016/j.cbpa.2015.10.009 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/ 10.1016/j.cbpa.2015.10.009 es_ES
dc.description.upvformatpinicio 98 es_ES
dc.description.upvformatpfin 106 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 191 es_ES
dc.relation.senia 295181 es_ES
dc.identifier.eissn 1531-4332
dc.identifier.pmid 26459984
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES


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