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The IBO germination quantitative trait locus encodes a phosphatase 2C-related variant with a nonsynonymous amino acid change that interferes with abscisic acid signaling

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The IBO germination quantitative trait locus encodes a phosphatase 2C-related variant with a nonsynonymous amino acid change that interferes with abscisic acid signaling

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dc.contributor.author Amiguet-Vercher, Amelia es_ES
dc.contributor.author Santuari, Luca es_ES
dc.contributor.author González Guzmán, Miguel es_ES
dc.contributor.author Depuydt, Stephen es_ES
dc.contributor.author Rodríguez Egea, Pedro Luís es_ES
dc.contributor.author Hardtke, Christian S. es_ES
dc.date.accessioned 2017-06-06T11:42:23Z
dc.date.available 2017-06-06T11:42:23Z
dc.date.issued 2015-02
dc.identifier.issn 0028-646X
dc.identifier.uri http://hdl.handle.net/10251/82420
dc.description.abstract [EN] Natural genetic variation is crucial for adaptability of plants to different environments. Seed dormancy prevents precocious germination in unsuitable conditions and is an adaptation to a major macro-environmental parameter, the seasonal variation in temperature and day length. Here we report the isolation of IBO, a quantitative trait locus (QTL) that governs c. 30% of germination rate variance in an Arabidopsis recombinant inbred line (RIL) population derived from the parental accessions Eilenburg-0 (Eil-0) and Loch Ness-0 (Lc-0).IBO encodes an uncharacterized phosphatase 2C-related protein, but neither the Eil-0 nor the Lc-0 variant, which differ in a single amino acid, have any appreciable phosphatase activity in in vitro assays. However, we found that the amino acid change in the Lc-0 variant of the IBO protein confers reduced germination rate. Moreover, unlike the Eil-0 variant of the protein, the Lc-0 variant can interfere with the activity of the phosphatase 2C ABSCISIC ACID INSENSITIVE 1 in vitro. This suggests that the Lc-0 variant possibly interferes with abscisic acid signaling, a notion that is supported by physiological assays. Thus, we isolated an example of a QTL allele with a nonsynonymous amino acid change that might mediate local adaptation of seed germination timing. es_ES
dc.language Inglés es_ES
dc.publisher Wiley es_ES
dc.relation.ispartof New Phytologist es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Abscisic acid (ABA) es_ES
dc.subject Arabidopsis thaliana es_ES
dc.subject IBO es_ES
dc.subject Natural variation es_ES
dc.subject Phosphatase 2C (PP2C) es_ES
dc.subject Quantitative trait locus (QTL) es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title The IBO germination quantitative trait locus encodes a phosphatase 2C-related variant with a nonsynonymous amino acid change that interferes with abscisic acid signaling es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/nph.13225
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Amiguet-Vercher, A.; Santuari, L.; Gonzalez Guzman, M.; Depuydt, S.; Rodríguez Egea, PL.; Hardtke, CS. (2015). The IBO germination quantitative trait locus encodes a phosphatase 2C-related variant with a nonsynonymous amino acid change that interferes with abscisic acid signaling. New Phytologist. 205(3):1076-1082. doi:10.1111/nph.13225 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1111/nph.13225 es_ES
dc.description.upvformatpinicio 1076 es_ES
dc.description.upvformatpfin 1082 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 205 es_ES
dc.description.issue 3 es_ES
dc.relation.senia 308246 es_ES
dc.identifier.eissn 1469-8137
dc.identifier.pmid 25490966


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