Zhao, J.; He, L.; Pan, L.; Liu, Y.; Yao, H.; Bao, G. (2017). Effect of a lytic bacteriophage on rabbits experimentally infected with pathogenic Escherichia coli. World Rabbit Science. 25(3):273-279. https://doi.org/10.4995/wrs.2017.6395
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/88258
Title:
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Effect of a lytic bacteriophage on rabbits experimentally infected with pathogenic Escherichia coli
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Author:
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Zhao, J.
He, L.
Pan, L.
Liu, Y.
Yao, H.
Bao, G.
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Issued date:
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Abstract:
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[EN] Pathogenic Escherichia coli (E. coli) is severely threatening the rabbit industry in China, and the concern over antibiotic-resistant bacteria has given rise to an urgent need for antibiotic alternatives. In this ...[+]
[EN] Pathogenic Escherichia coli (E. coli) is severely threatening the rabbit industry in China, and the concern over antibiotic-resistant bacteria has given rise to an urgent need for antibiotic alternatives. In this study, a member (ZRP1) of the Myoviridae family was isolated from rabbit faeces using a strain of rabbit atypical enteropathogenic E. coli (ZR1) as host. The one-step growth curve indicated that the latent period was around 25 to 30 min and the burst size was 144±31 plaque-forming unit/cell. The rate of phage-resistant mutation was 7×10–5±4×10–5. When the bacteriophage input at the multiplicity of infection (MOI) was 0.1, 1 or 10, the growth of host E. coli in broth was inhibited for 5 h. A single intravenous injection of ZRP1 at MOI 0.1, 1 or 10 significantly prolonged the survival time of rabbits which simultaneously received a lethal dose of ZR1.
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Subjects:
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Atypical enteropathogenic Escherichia coli
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Bacteriophage
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Phage therapy
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Rabbit
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Copyrigths:
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Reserva de todos los derechos
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Source:
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World Rabbit Science. (issn:
1257-5011
) (eissn:
1989-8886
)
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DOI:
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10.4995/wrs.2017.6395
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Publisher:
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Universitat Politècnica de València
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Publisher version:
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https://doi.org/10.4995/wrs.2017.6395
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Project ID:
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info:eu-repo/grantAgreement/Earmarked Fund for Modern Agro-industry Technology Research System//CARS-44-C-2/
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Thanks:
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This work was supported by a grant from the National Rabbit Industry Technology System of P. R. China (CARS44-C-2).
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Type:
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Artículo
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