Fraga Timiraos, Ana BelénLhiaubet, Virginie LyriaMiranda Alonso, Miguel Ángel2017-10-022017-10-022016-05-101433-7851https://riunet.upv.es/handle/10251/88383[EN] Photolyases are intriguing enzymes that take advantage of sunlight to restore lesions like cyclobutane pyrimidine dimers or (6-4) photoproducts. This work focused on the photoreductive process responsible for splitting of the azetidine ring proposed to occur during (6-4) photoproduct repair at a thymine-cytosine sequence. A model compound formed by photocycloaddition between thymine and 6-azauracil has been designed to mimic the elusive azetidine intermediate. The photoinduced electron transfer process has been investigated by means of steady-state and time-resolved fluorescence using photosensitizers with oxidation potentials in the singlet excited state ranging from -3.3 to -2.1V vs. SCE. Azetidine ring splitting and recovery of repaired bases were proven by HPLC analysis.Reserva de todos los derechos6-azauracilcycloreversionDNA damagefluorescencephotosensitizationQUIMICA ORGANICAArtículo10.1002/anie.201601475Abierto1521-377327061458