Marqués, María-CarmenRuiz, RaúlMontagud-Martínez, RoserMárquez-Costa, RosaAlbert, SandraDomingo-Calap, PilarRodrigo Tarrega, Guillermo2022-05-112022-05-112021-12-17https://riunet.upv.es/handle/10251/182539[EN] The novel respiratory virus SARS-CoV-2 is rapidly evolving across the world with the potential of increasing its transmission and the induced disease. Here, we applied the CRISPR-Cas12a system to detect, without the need of sequencing, SARS-CoV-2 genomes harboring the E484K mutation, first identified in the Beta variant and catalogued as an escape mutation. The E484K mutation creates a canonical protospacer adjacent motif for Cas12a recognition in the resulting DNA amplicon, which was exploited to obtain a differential readout. We analyzed a series of fecal samples from hospitalized patients in Valencia (Spain), finding one infection with SARS-CoV-2 harboring the E484K mutation, which was then confirmed by sequencing. Overall, these results suggest that CRISPR diagnostics can be a useful tool in epidemiology to monitor the spread of escape mutations.Reserva de todos los derechosCRISPR diagnosticsEpidemiological surveillanceVirus evolutionArtículo10.1021/acssynbio.1c00323Abierto2161-506334783536PMC8610009