Alonso Molina, José LuísAmoros, InmaculadaCañigral Cárcel, Irene2020-04-012020-04-0120110175-7598https://riunet.upv.es/handle/10251/139922[EN] Cryptosporidium and Giardia are major causes of diarrheal disease in humans worldwide and are major causes of protozoan waterborne diseases. Two DNA TaqMan PCR-based Giardia and Cryptosporidium methods targeting a 74-bp sequence of the ß-giardin Giardia gene and a 151-bp sequence of the COWP Cryptosporidium gene, respectively, were used as models to compare two different LNA/DNA TaqMan probes to improve the detection limit in a real-time PCR assay. The LNA probes were the most sensitive resulting in 0.96 to 1.57 lower C t values than a DNA Giardia TaqMan probe and 0.56 to 2.21 lower than a DNA Cryptosporidium TaqMan probe. Evaluation of TaqMan Giardia and Cryptosporidium probes with LNA substitutions resulted in real-time PCR curves with an earlier C t values than conventional DNA TaqMan probes. In conclusion, the LNA probes could be useful for more sensitive detection limits. © 2011 Springer-Verlag.Reserva de todos los derechosCryptosporidiumGiardiaLightCyclerLNA probeQPCRSewageTaqManDNAGenesNucleic acidsProbesPolymerase chain reactionAssayDiarrheal diseaseGene expressionGenetic analysisProtozoanSensitivity analysisSpecies diversityWaterborne diseaseArticleDNA extractionGene sequenceNonhumanNucleic acid probeQuantitative analysisReal time polymerase chain reactionSamplingCytoskeletal ProteinsHumansOligonucleotide ProbesParasitologyProtozoan ProteinsSensitivity and SpecificityProtozoaArtículo10.1007/s00253-010-2984-6Abierto