Marques, M. CarmenPEREZ AMADOR, MIGUEL ANGEL2017-06-222017-06-222012978-1-61779-423-0978-1-61779-424-71064-3745https://riunet.upv.es/handle/10251/83428[EN] We have developed an integrated method to generate a normalized cDNA collection enriched in full-length and rare transcripts from citrus, using different species and multiple tissues and developmental stages. Interpretation of ever-increasing raw sequence information generated by modern genome sequencing technologies faces multiple challenges, such as gene function analysis and genome annotation. In this regard, the availability of full-length cDNA clones facilitates functional analysis of the corresponding genes enabling manipulation of their expression and the generation of a variety of tagged versions of the native protein. The development of full-length cDNA sequences has the power to improve the quality of genome annotation, as well as provide tools for functional characterization of genesReserva de todos los derechosLibrarycDNACitrusFull-lengthNormalizedSMARTDSN nucleaseGateway technologyConstruction and analysis of full-length and normalized cDNA libraries from citrusArtÃculo10.1007/978-1-61779-424-7_5Abierto22130983