Cuesta Amat, GonzaloSoler Hernández, AlbertAlonso Molina, José LuísRuvira, M.A.Lucena, T.Arahal, D.R.Goodfellow, M.2014-09-182014-09-182013-010003-6072https://riunet.upv.es/handle/10251/39737A novel actinomycete, designated PA3T, was isolated from an oil refinery wastewater treatment plant, located in Palos de la frontera, Huelva, Spain, and characterized taxonomically by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate formed a distinct subclade in the Pseudonocardia tree together with Pseudonocardia asaccharolytica DSM 44247T. The chemotaxonomic properties of the isolate, for example, the presence of MK-8 (H4) as the predominant menaquinone and iso-C16:0 as the major fatty acid are consistent with its classification in the genus Pseudonocardia. DNA:DNA pairing experiments between the isolate and the type strain of P. asaccharolytica DSM 44247T showed that they belonged to separate genomic species. The two strains were readily distinguished using a combination of phenotypic properties. Consequently, it is proposed that isolate PA3T represents a novel species for which the name Pseudonocardia hispaniensis sp. nov. is proposed. The type strain is PA3T (= CCM 8391T = CECT 8030T).Reserva de todos los derechosPseudonocardia hispaniensis sp. novSystematicsPolyphasic taxonomyActivated sludgeMICROBIOLOGIAArtículo10.1007/s10482-012-9792-1Abierto