Hernandez-Contreras, JordiJordi Roig-RubioPARRA ALVAREZ, MARGARITAGil, SalvadorArroyo, PauSáez, Jose A.Lodeiro, CarlosGaviña, Pablo2025-05-142025-05-142025-050026-3672https://riunet.upv.es/handle/10251/220946[EN] The illicit use of gamma-hydroxybutyrate (GHB) in drug-facilitated crimes underscores the urgent need for rapid and reliable detection technologies. This study introduces two innovative silica-based nanosensors that offer real-time, on-site detection of GHB in spiked beverages at concentrations typical of chemical submission cases. Both chemosensors are based on silica nanoparticles functionalized with 2-aminonaphtoxazole derivatives. The first nanosensor, modified with a p-nitroaniline chromogenic unit, enables a visible color change for immediate visual identification, while the second incorporates a fluorescein derivative for high-sensitivity detection via fluorescence. Both nanosystems demonstrate exceptional specificity to GHB, with minimal interference from other substances. Moreover, the solid-phase integration of these nanosensors onto hydrophobic cellulose-based substrates enhances their portability and user-friendliness, making them suitable for nightlife venues and forensic applications. Finally, chromogenic precursor 1 has proven to be an excellent chemosensor for the visual detection of GHB in oral fluid, with a calculated LOD of 19.2 mu M, and a linear response in the 32-132 mu M range.Reconocimiento (by)Chemical submissionSilica nanoparticlesChromo-fluorogenic chemosensorsGamma-hydroxybutyrate (GHB)Oral fluidsSpiked drinksArtículo10.1007/s00604-025-07134-9Abierto40175785PMC11965150