This README.txt file was generated on 2022-11-16 by Juan Carlos Marín-Payá ------------------- GENERAL INFORMATION ------------------- Title of Dataset: Protein-Functionalized Microgel for Multiple Myeloma Cells’ 3D Culture Author Information Principal Investigator: Juan Carlos Marín-Payá; 1; juancarlosmarinpaya@gmail.com Associate or Co-investigator: Sandra Clara Trujillo; 1,2; sanclatr@gmail.com; https://orcid.org/0000-0002-6455-2993 Lourdes Cordón; 2,3; lou.cordon@gmail.com; https://orcid.org/0000-0002-8808-3423 Gloria Gallego Ferrer; 1,2; ggallego@ter.upv.es; https://orcid.org/0000-0002-2428-0903 Amparo Sempere; sempere_amp@gva.es José Luís Gómez Ribelles; 1,2; jlgomez@ter.upv.es; https://orcid.org/0000-0001-9099-0885 Affiliations: 1 Centre for Biomaterials and Tissue Engineering, CBIT, Universitat Politècnica de València, 46022 Valencia, Spain 2 Biomedical Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28029 Valencia, Spain 3 Centro de Investigación Biomédica en Red de Cáncer (CIBERONC), Instituto Carlos III, 20029 Madrid, Spain 4 Hematology Research Group, Instituto de Investigación Sanitaria La Fe (IISLAFE), 46026 Valencia, Spain 5 Haematology Department, Hospital Universitari i Politècnic La Fe, 46026 Valencia, Spain Date of data collection: From 2018-01-01 to 2020-01-01 Geographic location of data collection: Centro de Biomateriales e Ingeniería Tisular, Universitat Politècnica de València, Valencia, Spain (39.478006, -0.333463); Universitat Politècnica de València, 46022 Valencia and Haematology Department, Hospital Universitari i Politècnic La Fe, 46026 Valencia, Spain Information about funding sources or sponsorship that supported the collection of the data: This research was funded by the Generalitat Valenciana, grant number PROMETEO/2016/063; José Luis Gómez Ribelles acknowledges that support by was funded by the Spanish State Research Agency (AEI) through the PID2019-106099RB-C41 / AEI / 10.13039/501100011033 project. General description: The excel documents contain the crude numerical data of all the quantitative outputs of the article "Protein-Functionalized Microgel for Multiple Myeloma Cells’ 3D Culture" published in November 2022 in the journal Biomedicines (MDPI). The title of each file identifies which test the contained data corresponds to. The data is obtained in order to characterise the microspheres and cell behaviour in the 3D cell culture platform. Keywords: multiple myeloma; microgel; fibronectin; dexamethasone; bortezomib. -------------------------- SHARING/ACCESS INFORMATION -------------------------- Open Access to data: Open Date end Embargo: non required Citation for and links to publications that cite or use the data: https://doi.org/10.3390/biomedicines10112797 -------------------- DATA & FILE OVERVIEW -------------------- File list: -BCA Results: Results of data obtained by BCA assay to quantify fibronectin in microspheres. -Viability assay RPMI 8226 cells days 2-5-7: Results of data obtained by flow cytometry on RPMI 8226 cell viability. -Viability assay U266 and MM.1S cells days 2-5-7: Results of data obtained by flow cytometry on U266 and MM.1S cells viability. -Proliferation assay U266 and MM.1S cells days 2-5-7: Results of data obtained by Picogreen assay of the cell number of U226 and MM.1S cells. -Proliferation assay RPMI-8226 days 2-5-7: Results of data obtained by Picogreen assay of the cell number of RPMI-8226 cell. -Drug assay RPMI 8226 cells: Results of data obtained by flow cytometry on cell mortality. -Cell cycle assay RPMI 8226 cells dys 2-3-5 Days: Results of data obtained by flow cytometry on cell cycle. -Size distribution 2.000 rpm: Results of data of the size of alginate microspheres after measuring them from electron microscopy images using ImageJ. -Size distribution 750 rpm: Results of data of the size of alginate microspheres after measuring them from electron microscopy images using ImageJ. -Size distribution 1.500 rpm: Results of data of the size of alginate microspheres after measuring them from electron microscopy images using ImageJ. Type of version of the dataset: Data and processed data Total size: 123,7 KB -------------------------- METHODOLOGICAL INFORMATION -------------------------- Description of methods used for collection/generation of data: https://doi.org/10.3390/biomedicines10112797 Methods for processing the data: The ANOVA one-way analysis (Bonferroni test) was used to determine statistically significant differences between groups by the IBM SPSS Statistics software version 20.0 considering p ≤ 0.05 significant. Software- or Instrument-specific information needed to interpret the data, including software and hardware version numbers: Flow cytometry: Kaluza 2.1 software PicoGreen and micro-BCA: Victor3 Plate Reader Microsphere’s visualization: FESEM, ULTRA 55 model, ZEISS