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Listar por autor "Flores Pedauye, Ricardo"

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A chloroplastic RNA ligase activity analogous to the bacterial and archaeal 2 '-5 ' RNA ligase

Molina Serrano, Diego; Marques Signes, Jorge; Nohales Zafra, Maria Angeles; Flores Pedauye, Ricardo; Daros Arnau, Jose Antonio (Taylor &amp; Francis: STM, Behavioural Science and Public Health Titles, 2012-03)

[EN] Bacteria and archaea contain a 2'-5' RNA ligase that seals in vitro 2',3'-cyclic phosphodiester and 5'-hydroxyl RNA termini, generating a 2',5'-phosphodiester bond. In our search for an RNA ligase able to circularize ...
A pospiviroid from symptomless portulaca plants closely related to iresine viroid 1

Verhoeven, J.TH J.; Roenhorst, JW; Hooftman, M; Meekes, E.T.M.; Flores Pedauye, Ricardo; Serra Alfonso, Pedro (Elsevier, 2015-07-02)

[EN] In symptomless plants of portulaca a potential new pospiviroid was characterized. Analysis by both double and return PAGE showed the presence of a circular RNA. RT-PCR and sequencing revealed a genome of 351 nt with ...
Apple hammerhead viroid-like RNA is a bona fide viroid: Autonomous replication and structural features support its inclusion as a new member in the genus Pelamoviroid

Serra Alfonso, Pedro; Messmer, A.; Sanderson, D.; James, D.; Flores Pedauye, Ricardo (Elsevier, 2018)

[EN] Apple hammerhead viroid-like RNA (AHVd RNA) has been reported in different apple cultivars and geographic regions and, considering the presence of hammerhead ribozymes in both polarity strands, suspected to be either ...
Callose depositionin plasmodesmata and viroid invasion of the shoot apical meristem

Flores Pedauye, Ricardo (Frontiers Media, 2016-02-02)
Caracterización de actividades RNA ligasa implicadas en la replicación de los viroides nucleares y cloroplásticos

Nohales Zafra, Maria Angeles (Universitat Politècnica de València, 2011-10-21)

Los viroides son pequeños RNAs circulares de cadena sencilla (246-401 nt) patógenos de plantas. A pesar de su pequeño tamaño y de no codificar proteínas, son capaces de replicarse autónomamente, moverse sistémicamente y, ...
Citrus tristeza virus co-opts glyceraldehyde 3-phosphate dehydrogenase for its infectious cycle by interacting with the viral-encoded protein p23

Ruiz Ruiz, Susana; Spano, R.; Navarro, Luis; Moreno, Pedro; Peña, L.; FLORES PEDAUYE, RICARDO (Springer-Verlag, 2018-11-03)

[EN] Key messageCitrus tristeza virus encodes a unique protein, p23, with multiple functional roles that include co-option of the cytoplasmic glyceraldehyde 3-phosphate dehydrogenase to facilitate the viral infectious ...
Citrus tristeza virus infection induces the accumulation of viral small RNAs (21- 24-nt) mapping preferentially at the 3 -terminal region of the genomic RNA and affects the host small RNA profile

Ruiz Ruiz, Susana; Navarro, Beatriz; Gisel, Andreas; Peña, Leandro; Navarro, Luis; Moreno, Pedro; Di Serio, Francesco; Flores Pedauye, Ricardo (Springer Verlag (Germany), 2011-04)

[EN] To get an insight into the host RNA silencing defense induced by Citrus tristeza virus (CTV) and into the counter defensive reaction mediated by its three silencing suppressors (p25, p20 and p23), we have examined by ...
Citrus tristeza virus p23: a unique protein mediating key virus-host interactions

Flores Pedauye, Ricardo; Ruiz Ruiz, Susana; Soler, Nuria; Sanchez Navarro, Jesus Angel; Fagoaga García, Carmen Concepción; López Del Rincón, Carmelo; Navarro Lucas, Luis; Moreno, Pedro; Peña, Leandro (Frontiers, 2013-05)

[EN] The large RNA genome of Citrus tristeza virus (CTV; ca. 20 kb) contains 12 open reading frames, with the 3 -terminal one corresponding to a protein of 209 amino acids (p23) that is expressed from an abundant ...
Citrus tristeza virus p23: determinants for nucleolar localization and their influence on suppression of RNA silencing and pathogenesis

Ruiz Ruiz, Susana; Soler, Nuria; Sanchez Navarro, Jesus Angel; Fagoaga, Carmen; López Del Rincón, Carmelo; Navarro Lucas, Luis; Moreno, Pedro; PEÑA GARCIA, LEANDRO; Flores Pedauye, Ricardo (American Phytopathological Society, 2013-03)

Citrus tristeza virus (CTV) encodes a singular protein (p23, 209 amino acids) with multiple functions, including RNA silencing suppression (RSS). Confocal laser-scanning microscopy of green fluorescent protein (GFP)-p23 ...
Citrus tristeza virus: Host RNA Silencing and Virus Counteraction

Ruiz-Ruiz, Susana; Navarro, Beatriz; PEÑA GARCIA, LEANDRO; Navarro, Luis; Moreno, Pedro; Di Serio, Francesco; FLORES PEDAUYE, RICARDO (Springer, 2019)

[EN] To dissect the host RNA silencing response incited by citrus tristeza virus (CTV, genus Closterovirus), a (+) ssRNA of similar to 19300 nt, and the counter reaction deployed by the virus via its three RNA silencing ...
Current status of viroid taxonomy

DI SERIO, F; Flores Pedauye, Ricardo; Verhoeven, J. Th. J.; Li, S-F.; Pallás Benet, Vicente; Randles, J. W.; Sano, T.; Vidalakis, G.; Owens, R. A. (Springer Verlag (Germany), 2014-12)

Viroids are the smallest autonomous infectious nucleic acids known so far. With a small circular RNA genome of about 250-400 nt, which apparently does not code for any protein, viroids replicate and move systemically in ...
Cytopathic effects incited by viroid RNAs and putative underlying mechanisms

Di Serio, Francesco; De Stradis, Angelo; Delgado Villar, Sonia Guadalupe; Flores Pedauye, Ricardo; Navarro, Beatriz (Frontiers Media, 2013-01-07)

[EN] Viroids are infectious agents identified only in plants so far. In contrast to viruses, the genome of viroids is composed of a tiny circular RNA (250-400 nt) not coding for proteins, but containing in its compact ...
Dahlia latent viroid: a recombinant new species of the family Pospiviroidae posing intriguing questions about its origin and classification

Verhoeven, Jacobus Th. J.; Meekes, Ellis T. M.; Roenhorst, Johanna W.; Flores Pedauye, Ricardo; Serra Alfonso, Pedro (Microbiology Society, 2013-04)

[EN] A viroid-like RNA has been detected in two asymptomatic dahlia accessions by return and double PAGE. It appeared smaller than Chrysanthemum stunt viroid and Potato spindle tuber viroid, the two members of the genus ...
Degradación in vivo de un viroide de replicación nuclear: rutas catalizadas por proteínas Argonauta cargadas con pequeños RNAs viroidales y por otras ribonucleasas que generan RNAs subgenómicos

Minoia, Sofia (Universitat Politècnica de València, 2015-03-31)

Los viroides, los agentes infecciosos más simples de la escala biológica, están constituidos por una molécula circular de RNA monocatenario de aproximadamente 250-400 nucleótios (nt) que no codifica proteína alguna. A ...
Different rates of spontaneous mutation of chloroplastic and nuclear viroids as determined by high-fidelity ultra-deep sequencing

López-Carrasco, María Amparo; Ballesteros Martínez, Cristina; Sentandreu, V.; Delgado Villar, Sonia Guadalupe; Gago Zachert, Selma Persida; FLORES PEDAUYE, RICARDO; Sanjuan Verdeguer, Rafael (Public Library of Science, 2017-09-14)

[EN] Mutation rates vary by orders of magnitude across biological systems, being higher for simpler genomes. The simplest known genomes correspond to viroids, subviral plant replicons constituted by circular non-coding ...
Direct visualization of the native structure of viroid RNAs at single-molecule resolution by atomic force microscopy

Moreno, M.; Vázquez, L.; López Carrasco, A.; Martín-Gago, J. A.; FLORES PEDAUYE, RICARDO; Briones, C. (Landes Bioscience, 2019)

[EN] Viroids are small infectious, non-protein-coding circular RNAs that replicate independently and, in some cases, incite diseases in plants. They are classified into two families: Pospiviroidae, composed of species that ...
Disección de la estructura secundaria in silico, in vitro e in vivo de RNAs viroidales nucleares y cloroplásticos

López Carrasco, María Amparo (Universitat Politècnica de València, 2017-09-01)

Viroids, small circular RNAs (246-401 nt) with a high content in secondary structure that until recently have been detected only in higher plants, are the simplest infectious agents in the biological scale and do not encode ...
Dissecting the secondary structure of the circular RNA of a nuclear viroid in vivo: A "naked" rod-like conformation similar but not identical to that observed in vitro

López-Carrasco, María Amparo; Flores Pedauye, Ricardo (Landes Bioscience, 2017)

[EN] With a minimal (250-400nt), non-protein-coding, circular RNA genome, viroids rely on sequence/structural motifs for replication and colonization of their host plants. These motifs are embedded in a compact secondary ...
Double-stranded RNA interferes in a sequence-specific manner with the infection of representative members of the two viroid families

CARBONELL, ALBERTO; Martínez de Alba, Ángel-Emilio; FLORES PEDAUYE, RICARDO; Gago, Selma (Elsevier, 2008-02-05)

[EN] Infection by viroids, non-protein-coding circular RNAs, occurs with the accumulation of 21¿24 nt viroid-derived small RNAs (vd-sRNAs) with characteristic properties of small interfering RNAs (siRNAs) associated to RNA ...
e-Book on Closteroviridae

Flores Pedauye, Ricardo; Moreno, Pedro; Falk, Bryce; Martelli, Giovanni P.; Dawson, William O. (Frontiers Media, 2013-12-27)