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Listar por autor "Daros Arnau, Jose Antonio"

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A chloroplastic RNA ligase activity analogous to the bacterial and archaeal 2 '-5 ' RNA ligase

Molina Serrano, Diego; Marques Signes, Jorge; Nohales Zafra, Maria Angeles; Flores Pedauye, Ricardo; Daros Arnau, Jose Antonio (Taylor &amp; Francis: STM, Behavioural Science and Public Health Titles, 2012-03)

[EN] Bacteria and archaea contain a 2'-5' RNA ligase that seals in vitro 2',3'-cyclic phosphodiester and 5'-hydroxyl RNA termini, generating a 2',5'-phosphodiester bond. In our search for an RNA ligase able to circularize ...
A potyvirus vector efficiently targets recombinant proteins to chloroplasts, mitochondria and nuclei in plant cells when expressed at the amino terminus of the polyprotein

Majer, Eszter; Navarro Bohigues, José Antonio; Daros Arnau, Jose Antonio (Wiley-VCH Verlag, 2015-11)

Plant virus-based expression systems allow quick and efficient production of recombinant proteins in plant biofactories. Among them, a system derived from tobacco etch virus (TEV; genus potyvirus) permits coexpression of ...
A Recombinant Potato virus Y Infectious Clone Tagged with the Rosea1 Visual Marker (PVY-Ros1) Facilitates the Analysis of Viral Infectivity and Allows the Production of Large Amounts of Anthocyanins in Plants

Cordero, Teresa; Mohamed, M.A.; Lopez Moya, J.J.; DAROS ARNAU, JOSE ANTONIO (Frontiers Media SA, 2017-04-06)

[EN] Potato virus Y (PVY) is a major threat to the cultivation of potato and other solanaceous plants. By inserting a cDNA coding for the Antirrhinum majus Rosea1 transcription factor into a PVY infectious clone, we created ...
A viroid-derived system to produce large amounts of recombinant RNA in Escherichia coli

DAROS ARNAU, JOSE ANTONIO; Aragones, V; Cordero-Cucart, María Teresa (Nature Publishing Group, 2018)

[EN] Viruses have been engineered into useful biotechnological tools for gene therapy or to induce the synthesis of products of interest, such as therapeutic proteins and vaccines, in animal and fungal cells, bacteria or ...
A Watermelon mosaic virus clone tagged with the yellow visual maker phytoene synthase facilitates scoring infectivity in melon breeding programs

Aragones, V; Pérez De Castro, Ana María; Cordero, Teresa; Cebolla Cornejo, Jaime; López Del Rincón, Carmelo; Picó Sirvent, María Belén; DAROS ARNAU, JOSE ANTONIO (Springer-Verlag, 2019)
An infectious cDNA clone of a radish-infecting Turnip mosaic virus strain

López-González, Silvia; Aragonés, Verónica; Daros Arnau, Jose Antonio; Sánchez, Flora; Ponz, Fernando (Springer Verlag (Germany), 2017-05)

Turnip Mosaic Virus (TuMV) is an economically important potyvirus for which hundreds of hosts have been reported, thus making it a rather exceptional case in the genus. Several viral infectious clones have been generated ...
Análisis de la dinámica de replicación de un virus de RNA de plantas y del uso de microRNAs artificiales como estrategia antiviral

Martínez García, Fernando (Editorial Universitat Politècnica de València, 2014-02-10)

Los virus de RNA de cadena positiva conforman el grupo más numeroso y diverso de los patógenos virales de las plantas. Se replican mediante un mecanismo en el que la RNA polimerasa RNA dependiente viral, junto con otras ...
Aplicaciones del factor de transcripción Rosea1 de la ruta de las antocianinas como marcador visual en virología molecular y biotecnología de plantas.

Cordero Cucart, Maria Teresa (Universitat Politècnica de València, 2021-07-19)

[ES] Los virus de plantas poseen la capacidad de parasitar células vegetales y poner a su disposición la maquinaria celular de la planta para la síntesis de sus propias proteínas. Aprovechando esta capacidad, la ingeniería ...
Artificial microRNAs and synthetic trans-acting small interfering RNAs interfere with viroid infection

Carbonell Olivares, Alberto; Daros Arnau, Jose Antonio (Wiley, 2016)

[EN] Artificial microRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs) are two classes of artificial small RNAs (sRNAs) engineered to silence endogenous transcripts as well as viral RNAs ...
Assessment of protein silver nanoparticles toxicity against pathogenic Alternaria solani

Abdel-Hafez, Sobhy I. I.; Nafady, Nivien A.; Abdel-Rahim, Ismail R.; Shaltout, Abeer M.; Daros Arnau, Jose Antonio; Mohamed, Mohamed A. (SpringerOpen, 2016-09-21)

Mycogenic synthesis of silver nanoparticles (AgNPs) was carried out in the present investigation using an aqueous extract of endophytic non-pathogenic Alternaria solani F10 (KT721914). The mycosynthesized AgNPs were ...
Avanzando hacía la edición genética CRISPR-Cas en lechuga mediante vectores virales

Cano Sales, Lucía (Universitat Politècnica de València, 2023-10-30)

[ES] La edición genética CRISPR-Cas ha abierto oportunidades sin precedentes en biotecnología de plantas. Para conseguir edición genética CRISPR-Cas en plantas es necesario expresar dos componentes de la reacción, una ...
Bacteria associated with the rhizosphere of manganese-deficient date palms affected by Brettle Leaf Disease

Marques, J.; Duran Vila, Nuria; Namsi, A.; Bove, J.M.; Daros Arnau, Jose Antonio (Edizioni ETS, 2012-03)

[EN] Brittle leaf disease or "Maladie des feuilles cassantes" (MFC) is a disorder affecting date palm (Phoenix dactylifera) orchards in oases of southern Tunisia. No causal pathogen has yet been identified, despite search ...
Biotechnological Application of Potyvirus-Based Vectors in Cucurbit Plants

Houhou, Fakhreddine (Universitat Politècnica de València, 2021-11-29)

[ES] Hoy en día, los virus de las plantas no sólo se perciben como patógenos, sino que también son capaces de establecer una asociación beneficiosa con sus huéspedes y colaborar desde un punto de vista biotecnológico. El ...
Boolean Computation in Plants Using Post-translational Genetic Control and a Visual Output Signal

Cordero-Cucart, María Teresa; Rosado, A.; Majer, Eszter; Jaramillo Rosales, Alfonso; Rodrigo Tarrega, Guillermo; Daros Arnau, Jose Antonio (American Chemical Society, 2018-10)

[EN] Due to autotrophic growing capacity and extremely rich secondary metabolism, plants should be preferred targets of synthetic biology. However, developments in plants usually run below those in other taxonomic groups. ...
Caracterización de actividades RNA ligasa implicadas en la replicación de los viroides nucleares y cloroplásticos

Nohales Zafra, Maria Angeles (Universitat Politècnica de València, 2011-10-21)

Los viroides son pequeños RNAs circulares de cadena sencilla (246-401 nt) patógenos de plantas. A pesar de su pequeño tamaño y de no codificar proteínas, son capaces de replicarse autónomamente, moverse sistémicamente y, ...
Caracterización y secuenciación de un aislado español del virus del mosaico del tomate

Arias Moreno, Joan (Universitat Politècnica de València, 2016-05-19)

[ES] El virus del mosaico del tomate (ToMV) es un virus de RNA perteneciente al género Tobamovirus que se encuentra distribuido por todo el mundo y causa importantes pérdidas económicas en cultivos de tomate (Solanum ...
Carotenoid fortification of zucchini fruits using a viral RNA vector

Fakhreddine-Houhou, Houhou; Martí-Botella, Mari Carmen; Cordero-Cucart, María Teresa; Aragones, V; Sáez-Sánchez, Cristina; Cebolla Cornejo, Jaime; Pérez De Castro, Ana María; Rodriguez-Concepcion, Manuel; Picó Sirvent, María Belén; Daròs, José-Antonio (John Wiley & Sons, 2022-05)

[EN] Background Carotenoids are health-promoting metabolites in livestock and human diets. Some important crops have been genetically modified to increase their content. Although the usefulness of transgenic plants to ...
Chrysanthemum stunt viroid in Brazil: survey, identification, biological and molecular characterization and detection methods

Gobatto, D.; Chaves, A.L.R.; Harakava, R.; Marque, J. M.; Daros Arnau, Jose Antonio; Eiras, M. (Edizioni ETS, 2014-03)

[EN] In Brazil, the ornamental flowers and plants market moves in the wholesale and retail more than two billion US dollars annually, and chrysanthemum stands out as one of the most valuable commercial species. The stunting ...
Clonación y expresión de proteínas del virus del bronceado del tomate (TSWV) en plantas de Nicotiana benthamiana mediante un vector de expresión derivado del virus del grabado del tabaco (TEV)

Tudela Martínez, Julia María (Universitat Politècnica de València, 2012-11-29)

[ES] El virus del bronceado del tomate (Tomato spotted wilt virus, TSWV) está considerado como una de las virosis más destructivas de la agricultura actual, siendo capaz de infectar a una gran variedad de cultivos ...
Complete sequence of three different biotypes of Tomato spotted wilt virus (wild type, tomato Sw-5 resistance-breaking and pepper Tsw resistance-breaking) from Spain

Debreczeni, Diana Elvira; López Del Rincón, Carmelo; Aramburu, José; Daros Arnau, Jose Antonio; Soler Aleixandre, Salvador; Galipienso Torregrosa, Luis; Falk, Bryce W.; Rubio Miguelez, Luis (Springer Verlag (Germany), 2015-08)

[EN] Tomato spotted wilt virus (TSWV) is worldwide spread and causes production losses in many important horticultural crops such as tomato and pepper. Breeding resistant cultivars has been the most successful method for ...