[EN] A multi-technique approach was used to study the changes occurring in European eel Anguilla anguilla ovaries during hormonally-induced vitellogenesis. Aside from classic techniques used to monitor the vitellogenic ...
[EN] Vitrification was successfully applied to the sperm of two fish species, the freshwater Eurasian perch (Perca fluviatilis) and marine European eel (Anguilla anguilla). Sperm was collected, diluted in species specific ...
Vilchez Olivencia, Maria Carmen(Universitat Politècnica de València, 2014-02-21)
[ES] Se ha evaluado el efecto de dos regímenes térmicos (constante y variable) aplicados durante la maduración sexual de hembras de anguila, en la calidad de las puestas y el desarrollo embrionario posterior. Se analizan ...
[EN] Positive effects of probiotics on fish reproduction have been reported in several species. In the present study, 40 male European eels were weekly treated with recombinant hCG for 9 weeks and with three different ...
[EN] Little is known about the role of fat and fatty acids in European eel spermatogenesis. The aim of this research was
to study the changes in fat content and to carry out a quantitative analysis of the fatty acid ...
Baeza Ariño, Rosa; Peñaranda, D.S.; Vilchez Olivencia, Maria Carmen; Tveiten, Helge; Pérez Igualada, Luz María; Asturiano Nemesio, Juan Francisco(Elsevier, 2015-01-01)
[EN] The present study was undertaken to evaluate the correlations between the fatty acids in the liver and testis and the plasma levels of the hormonal steroids used during eel spermatogenesis, in order to clarify the ...
Vilchez Olivencia, Maria Carmen; D. Pla; Gallego Albiach, Victor; Sanz, Libia; Pérez Igualada, Luz María; Asturiano Nemesio, Juan Francisco; Calvete, Juan J.; Peñaranda, D.S.(Elsevier, 2016-11)
[EN] By first time, 2DE protein profile of European eel seminal plasma has been determined. 14 different proteins corresponding to 9 major families were identified in seminal plasma, through hormonal treatment. Some of ...
Vílchez Olivencia, María del Carmen(Universitat Politècnica de València, 2017-11-03)
In general, fish spermatozoa are immotile in the testis. Movement is activated due to the
osmotic shock (hypo- or hyperosmotic, depending on fish origin: freshwater or sea water
species) experienced when they are released ...
[EN] Reproduction in captivity is a key study issue in Anguilla anguilla as a possible solution for its dwindling population. Understanding the mechanisms controlling the production of ribosomal building blocks during ...
[EN] Characterization of all the progestin receptor genes (PRs) found in the European eel has been performed. There were five membrane PRs (mPRs): mPR alpha (alpha), mPRAL1 (alpha-likel), mPRAL2 (alpha-like2), mPRy (gamma), ...
[EN] This study looks at the correlations that fatty acids have with different tissues in the European eel (Anguilla anguilla L.) during hormonally-induced sexual maturation, with different sperm quality parameters. In ...
Pérez Igualada, Luz María; Vilchez Olivencia, Maria Carmen; Gallego Albiach, Victor; MORINI, MARINA; Peñaranda, D.S.; Asturiano Nemesio, Juan Francisco(Elsevier, 2016-01)
[EN] Sperm from European eel males treated with hCG(rec), was washed in a calcium free extender, and sperm motility was activated both in the presence (seawater, SW) and in the absence of calcium (NaCI + EDTA), and treated ...
Vilchez Olivencia, Maria Carmen; MORINI, MARINA; Peñaranda, D.S.; Gallego Albiach, Victor; Asturiano Nemesio, Juan Francisco; Pérez Igualada, Luz María(Elsevier, 2017)
[EN] The role of potassium from the seminal plasma and/or the activation media was examined by selectively removing from this media, and by testing the use of channel inhibitors and a K-ionophore. Sperm motility was measured ...
Vilchez Olivencia, Maria Carmen; MORINI, MARINA; Peñaranda, D.S.; Gallego Albiach, Victor; Asturiano Nemesio, Juan Francisco; Pérez Igualada, Luz María(Elsevier, 2016-08)
[EN] The role of seminal plasma sodium and activation media sodium on sperm motility was examined by selectively removing the element from these two media, in European eel sperm. Sperm size (sperm head area) was also ...
Gallego Albiach, Victor; Carneiro, P. C. F.; Mazzeo, Ilaria; Vilchez Olivencia, Maria Carmen; Peñaranda, D.S.; Soler, C.; Pérez Igualada, Luz María; Asturiano Nemesio, Juan Francisco(Elsevier, 2013-04-15)
[EN] The development of powerful computer-assisted sperm analysis software has made kinetic studies of spermatozoa possible. This system has been used and validated for several species, but some technical questions have ...
Peñaranda, D.S.; MORINI, MARINA; Tveiten, Helge; Vilchez Olivencia, Maria Carmen; Gallego Albiach, Victor; Dirks, Ron; van den Thillart, Guido E.E.J.M.; Pérez Igualada, Luz María; Asturiano Nemesio, Juan Francisco(Elsevier, 2016-07)
[EN] This study evaluates the effects of temperature on hCG-induced spermatogenesis in European eel (Anguilla anguilla), subjected to three thermal regimes: T10: 10 degrees C (first 4 weeks), 15 degrees C (next 3 weeks) ...
[EN] Estradiol (E-2) can bind to nuclear estrogen receptors (ESR) or membrane estrogen receptors (GPER). While mammals possess two nuclear ESRs and one membrane GPER, the European eel, like most other teleosts, has three ...
Gallego Albiach, Victor; Vilchez Olivencia, Maria Carmen; Peñaranda, D.S.; Pérez Igualada, Luz María; Herraez, María Paz; Asturiano Nemesio, Juan Francisco; Martinez-Pastor, Felipe(CSIRO Publishing, 2015)
[EN] There has been a marked reduction in natural stocks of eels (genus Anguilla) over the past 60 years, and the culture of eels is still based on the capture of very large quantities of juveniles. It is necessary to close ...
[EN] Activation at fertilization of the vertebrate egg is triggered by Ca2+ waves. Recent studies suggest the phospholipase C zeta (PLC zeta), a sperm-specific protein, triggers egg activation by an 1P3-mediated Ca2+ release ...