Ballester Beltrán, José; Trujillo, Sara; Alakpa, Enateri V.; Compañ Moreno, Vicente; Gavara, Rafael; Meek, Dominic; West, Christopher C.; Peault, Bruno; Dalby, Matthew J.; Salmerón Sánchez, Manuel(American Chemical Society, 2017)
[EN] Sandwichlike (SW) cultures are engineered as a multilayer technology to simultaneously stimulate dorsal and ventral cell receptors, seeking to mimic cell adhesion in three-dimensional (3D) environments in a reductionist ...
Cells behave differently between bidimensional (2D) and tridimensional (3D) environments. While most of the in vitro cultures are 2D, most of the in vivo extracellular matrices are 3D, which encourages the development of ...
While most of the in vivo extracellular matrices are 3D, most of the in vitro cultures are 2D--where only ventral adhesion is permitted--thus modifying cell behavior as a way to self-adaptation to this unnatural environment. ...
[EN] Fibronectin (FN) assembles into fibrillar networks by cells through an integrin-dependent mechanism. We have recently shown that simple FN adsorption onto poly(ethyl acrylate) surfaces (PEA), but not control polymer ...
Ballester Beltrán, José; Moratal Pérez, David; Lebourg, Myriam Madeleine; Salmerón Sánchez, Manuel(Royal Society of Chemistry, 2014)
[EN] Conventional 2D substrates fail to represent the natural environment of cells surrounded by the 3D extracellular matrix (ECM). We have proposed sandwich-like microenvironments as a versatile tool to study cell behaviour ...
Ballester Beltrán, José(Universitat Politècnica de València, 2011-11-11)
Se ha demostrado ampliamente que la adsorción de proteínas y la adhesión celular dependen de las características físico-químicas de los materiales. Sin embargo, pocas veces se ha estudiado la respuesta celular en materiales ...
Currently, cell culture systems that include nanoscale topography are widely used in order to provide cells additional cues closer to the in vivo environment, seeking to mimic the natural extracellular matrix. Electrospinning ...
Ballester Beltrán, José; Rico Tortosa, Patricia María; Moratal Pérez, David; Song, Wenlong; Mano, Joao F; Salmerón Sánchez, Manuel(Royal Society of Chemistry, 2011)
Protein adsorption and cellular behavior depend strongly on the wettability of substrates. Such studies are scarce for surfaces exhibiting extreme values of contact angles. Fibronectin (FN) adsorption and adhesion of ...
Background: The cell-material interaction is a complex bi-directional and dynamic process that mimics to a certain extent the natural interactions of cells with the extracellular matrix. Cells tend to adhere and rearrange ...
Ballester Beltrán, José(Universitat Politècnica de València, 2015-03-20)
Abstract
While most of the in vitro cultures are carried out on bi-dimensional (2D)
substrates, most of the in vivo extracellular matrices are threedimensional
(3D). Consequently cells behave differently on 2 ...