Seguí-Simarro, Jose M.; Corral Martínez, Patricia; Corredor, E.; Raska, I.; Testillano, P. S.; Risueno, M.C.(Elsevier, 2011-05-15)
After a stress treatment, in vitro-cultured pollen changes its normal gametophytic developmental pathway towards embryogenesis producing multicellular embryos from which, finally, haploid and double haploid plants develop. ...
Seguí-Simarro, Jose M.; Corral Martínez, Patricia; Parra Vega, Verónica; Gonzalez Garcia, Beatriz(Springer Verlag (Germany), 2011-05)
[EN] Tomato, eggplant, and pepper are three solanaceous crops of outstanding importance worldwide. For hybrid seed production in these species, a fast and cheap method to obtain pure (homozygous) lines is a priority. ...
Camacho-Fernández, Carolina; Seguí-Simarro, Jose M.; Mir Moreno, Ricardo; Boutilier, Kim; Corral-Martínez, Patricia(Frontiers Media SA, 2021-10-06)
[EN] Microspore cultures generate a heterogeneous population of embryogenic structures that can be grouped into highly embryogenic structures [exine-enclosed (EE) and loose bicellular structures (LBS)] and barely embryogenic ...
Sanz-Barrio, R.; Fernández-San Millán, A.; Carballeda, J.; Corral Martínez, Patricia; Seguí-Simarro, Jose M.; Farran, I.(Oxford University Press (OUP): Policy B - Oxford Open Option A, 2012)
Thioredoxins (Trxs) are ubiquitous disulphide reductases that play important roles in the redox regulation of many cellular processes. However, some redox-independent functions, such as chaperone activity, have also been ...
Corral Martínez, Patricia; Driouich, Azeddine; Seguí-Simarro, Jose M.(Frontiers Media SA, 2019-03-28)
[EN] Microspore embryogenesis is a manifestation of plant cell totipotency whereby new cell walls are formed as a consequence of the embryogenic switch. In particular, the callose-rich subintinal layer created immediately ...
Muñoz Vidal, Eva Luna(Universitat Politècnica de València, 2020-09-07)
[ES] La creciente demanda de productos vegetales de calidad en los próximos años hace que el perfeccionamiento de las estrategias empleadas en la Mejora Genética Vegetal para obtener líneas puras sea un campo de investigación ...
Viqueira Díaz-Alejo, Lucía(Universitat Politècnica de València, 2020-09-10)
[ES] El aumento de la población humana previsto en los próximos años, irá acompañado por una demanda mayor de productos vegetales. Esta necesidad de un aumento de producción se verá acompañada por el impacto del cambio ...
[EN] Notwithstanding the importance of eggplant in global horticulture, doubled haploid production in this species is still far from being efficient. Although acknowledged to have a role in the efficiency of androgenesis ...
Corral Martínez, Patricia; Seguí-Simarro, José M.(Springer Verlag (Germany), 2012)
Production of doubled haploids (DHs) through androgenesis induction is an important biotechnological tool for plant breeding. In some species, DHs are efficiently obtained through embryogenesis from isolated microspore ...
Rivas-Sendra, Alba; Corral Martínez, Patricia; Porcel, R.; Camacho-Fernández, Carolina; Calabuig-Serna, Antonio; Seguí-Simarro, Jose M.(Oxford University Press, 2019-02-01)
[EN] Microspore embryogenesis is an experimental morphogenic pathway with important applications in basic research and applied plant breeding, but its genetic, cellular, and molecular bases are poorly understood. We applied ...
Induction of secondary embryogenesis on transformed androgenic microspore-derived embryos (MDEs) is a convenient approach to avoid chimerism and hemizygosis for the introduced transgene. In this work, we improved two aspects ...
Parra Vega, Verónica; Corral Martínez, Patricia; Rivas-Sendra, Alba; Seguí-Simarro, Jose M.(Frontiers Media, 2015-02)
[EN] The change in developmental fate of microspores reprogrammed toward embryogenesis
is a complex but fascinating experimental system where microspores undergo dramatic
changes derived from the developmental switch. ...
In plant breeding, androgenic doubled haploids represent powerful tools to save time and resources for pure line generation. While in many species efficient protocols are known, in tomato (Solanum lycopersicum), the knowledge ...
[EN] Doubled haploid (DH) technology allows for
the production of pure lines, useful for plant breeding,
through a one-generation procedure that reduces considerably
the time and resources needed to produce them.
...
Parra Vega, Verónica; Corral Martínez, Patricia; Rivas-Sendra, Alba; Seguí-Simarro, Jose M.(Frontiers Media, 2015-11-25)
The induction of microspore embryogenesis produces dramatic changes in different aspects of the cell physiology and structure. Changes at the cell wall level are among the most intriguing and poorly understood. In this ...
Camacho Fernández, Carolina(Universitat Politècnica de València, 2021-03-08)
[ES] Los dobles haploides son una gran herramienta para la mejora genética de híbridos debido a que se puede alcanzar la homocigosis completa en una sola generación. Entre las técnicas usadas para obtener estas plantas, ...
Corral Martínez, Patricia; Parra Vega, Verónica; Seguí-Simarro, Jose M.(Oxford University Press (OUP): Policy B - Oxford Open Option A, 2013)
[EN] Induction of embryogenesis from isolated microspore cultures is a complex experimental system where microspores undergo dramatic changes in developmental fate. After ~40 years of application of electron microscopy to ...
Corral Martínez, Patricia(Universitat Politècnica de València, 2011-07-28)
La producción de doble haploides a través del cultivo de microsporas aisladas es una
alternativa para obtener líneas puras mucho más eficaz que las técnicas de mejora genética
clásica. Esta técnica presenta las ventajas ...
Rivas Sendra, Alba(Universitat Politècnica de València, 2013-02-25)
[ES] Las líneas puras son la base de la producción de semilla híbrida. La producción de doble haploides es una metodología alternativa a las técnicas de mejora genética clásica, que reduce considerablemente el tiempo y los ...
Corral Martínez, Patricia(Universitat Politècnica de València, 2013-09-02)
La androgénesis es un proceso experimental que permite la obtención de líneas puras doble haploides a través de embriogénesis o callogénesis inducida partiendo, en la mayoría de los casos, del gametófito masculino (polen ...