Sarrión Perdigones, Manuel Alejandro(Universitat Politècnica de València, 2014-02-07)
The post-genomics era has put at the disposal of modern plant breeders an endless list of genetic building blocks for the design of new biotechnological crops. After a first wave of single-gene transgenic with controversial ...
Sarrión Perdigones, Manuel Alejandro; Juárez Ortega, Paloma; Fernández Del Carmen, María Asunción; Granell Richart, Antonio; Orzáez Calatayud, Diego Vicente(Public Library of Science, 2011)
Synthetic Biology requires efficient and versatile DNA assembly systems to facilitate the building of new genetic modules/pathways from basic DNA parts in a standardized way. Here we present GoldenBraid (GB), a standardized ...
[EN] Many synthetic biologists have adopted methods based on Type IIS restriction enzymes and Golden Gate technology in their cloning procedures, as these enable the combinatorial assembly of modular elements in a very ...