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Listar por autor "Carbonell Olivares, Alberto"

Mostrando ítems 1-20 de 46

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A viral suppressor of RNA silencing inhibits ARGONAUTE 1 function by precluding target RNA binding to pre-assembled RISC

Kenesi, Erzsebet; CARBONELL, ALBERTO; Lozsa, Rita; Vertessy, Beata; Lakatos, Lorant (Oxford University Press, 2017-07-27)

[EN] In most eukaryotes, RNA silencing is an adaptive immune system regulating key biological processes including antiviral defense. To evade this response, viruses of plants, worms and insects have evolved viral suppressors ...
Antiviral roles of plant ARGONAUTES

CARBONELL, ALBERTO; Carrington, James C. (Elsevier, 2015-10)

[EN] ARGONAUTES (AGOs) are the effector proteins functioning in eukaryotic RNA silencing pathways. AGOs associate with small RNAs and are programmed to target complementary RNA or DNA. Plant viruses induce a potent and ...
Argonaute PIWI domain and microRNA duplex structure contribute to small RNA sorting in Arabidopsis.

Zhang, Xiaoming; Niu, DongDong; CARBONELL, ALBERTO; Wang, Airong; Lee, Angel; Tun, Vinnary; Wang, Zonghua; Carrington, James C.; Chang, Chia-en A.; Jin, Hailing (Nature Publishing Group, 2014-11-16)

[EN] Small RNAs (sRNAs) are loaded into ARGONAUTE (AGO) proteins to induce gene silencing. In plants, the 5¿-terminal nucleotide is important for sRNA sorting into different AGOs. Here we show that microRNA (miRNA) duplex ...
Artificial microRNAs and synthetic trans-acting small interfering RNAs interfere with viroid infection

Carbonell Olivares, Alberto; Daros Arnau, Jose Antonio (Wiley, 2016)

[EN] Artificial microRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs) are two classes of artificial small RNAs (sRNAs) engineered to silence endogenous transcripts as well as viral RNAs ...
Artificial Small RNA-Based Silencing Tools for Antiviral Resistance in Plants

Cisneros, Adriana E.; CARBONELL, ALBERTO (MDPI, 2020-06)

[EN] Artificial small RNAs (art-sRNAs), such as artificial microRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs), are highly specific 21-nucleotide small RNAs designed to recognize and silence ...
Diagnostics of Infections Produced by the Plant Viruses TMV, TEV, and PVX with CRISPR-Cas12 and CRISPR-Cas13

Marqués, María-Carmen; Sánchez-Vicente, Javier; Ruiz, Raúl; Montagud-Martínez, Roser; Márquez-Costa, Rosa; Gómez, Gustavo; CARBONELL, ALBERTO; Daròs, José-Antonio; Rodrigo Tarrega, Guillermo (American Chemical Society, 2022-07-15)

[EN] Viral infections in plants threaten food security. Thus, simple and effective methods for virus detection are required to adopt early measures that can prevent virus spread. However, current methods based on the ...
Dicer-like 4 is involved in restricting the systemic movement of Zucchini yellow mosaic virus in Nicotiana benthamiana

Cordero-Cucart, María Teresa; Cerdán García, Lidia; Carbonell Olivares, Alberto; Katsarou, Konstantina; Kalantidis, Kriton; Daros Arnau, Jose Antonio (American Phytopathological Society, 2016-01)

[EN] Zucchini yellow mosaic virus (ZYMV) induces serious diseases in cucurbits. To create a tool to screen for resistance genes, we cloned a wild ZYMV isolate and inserted the visual marker Roseal to obtain recombinant ...
Diverse amino acid changes at specific positions in the N-terminal region of the coat protein allow Plum pox virus to adapt to new hosts

CARBONELL, ALBERTO; Maliogka, Varvara I.; Pérez, José de Jesús; Salvador, Beatriz; San Leon, David; Garcia, Juan Antonio; Simon-Mateo, Carmen (Scientific Societies, 2013-10)

[EN] Plum pox virus (PPV)-D and PPV-R are two isolates from strain D of PPV that differ in host specificity. Previous analyses of chimeras originating from PPV-R and PPV-D suggested that the N terminus of the coat protein ...
Double-stranded RNA interferes in a sequence-specific manner with the infection of representative members of the two viroid families

CARBONELL, ALBERTO; Martínez de Alba, Ángel-Emilio; FLORES PEDAUYE, RICARDO; Gago, Selma (Elsevier, 2008-02-05)

[EN] Infection by viroids, non-protein-coding circular RNAs, occurs with the accumulation of 21¿24 nt viroid-derived small RNAs (vd-sRNAs) with characteristic properties of small interfering RNAs (siRNAs) associated to RNA ...
Downregulation of Tomato STEROL GLYCOSYLTRANSFERASE 1 Perturbs Plant Development and Facilitates Viroid Infection

Cisneros, Adriana E.; Lisón, Purificación; Campos, Laura; López-Tubau, Joan Manel; Altabella, Teresa; Ferrer, Albert; Daròs, José-Antonio; CARBONELL, ALBERTO (Oxford University Press, 2022)

[EN] Potato spindle tuber viroid (PSTVd) is a plant pathogen naturally infecting economically important crops such as tomato (Solanum lycopersicum). Here, we aimed to engineer tomato plants highly resistant to PSTVd and ...
Efecto del apareamiento de los nucleótidos del precursor shc-MIR390 sobre la acumulación de microRNAs artificiales derivados del mismo

García Cano, Pedro José (Universitat Politècnica de València, 2024-02-14)

[ES] El silenciamiento génico mediado RNA o RNA de interferencia (RNAi) es un mecanismo de regulación de la expresión génica tanto en células animales como vegetales. El RNAi está mediado por pequeños RNAs (sRNAs) no ...
Effects of the trinucleotide preceding the self-cleavage site on eggplant latent viroid hammerheads: differences in co- and post-transcriptional self-cleavage may explain the lack of trinucleotide AUC in most natural hammerheads

CARBONELL, ALBERTO; La Peña Del Rivero, Marcos De; FLORES PEDAUYE, RICARDO; Gago, Selma (Oxford University Press, 2006-11)

[EN] Eggplant latent viroid (ELVd) can form stable hammerhead structures in its (+) and (¿) strands. These ribozymes have the longest helices I reported in natural hammerheads, with that of the ELVd (+) hammerhead being ...
Expresión de pequeños RNAs artificiales en Nicotiana benthamiana mediante el virus del arrugamiento de la hoja de la col

Ortega Miralles, Iván (Universitat Politècnica de València, 2023-02-08)

[ES] El silenciamiento génico o RNA de interferencia (RNAi) ha sido descrito como un medio para suprimir la actividad de un gen tanto en plantas como en animales, y está basado en pequeños RNAs (sRNAs) de 20-35 nucleótidos ...
Factores del huésped implicados en el ciclo infectivo de patógenos de plantas

Beceiro Durán, Cristina (Universitat Politècnica de València, 2017-02-28)

[EN] Plant viruses can be manipulate d and converted into biotechnological tools to produce recombinant proteins in biofactory plan t s. One of the viruses most frequently used is Tobacco mosaic ...
Fast-forward Identification of Highly Effective Artificial Small RNAs Against Different Tomato spotted wilt virus Isolates

Carbonell, Alberto; López Del Rincón, Carmelo; Daròs, José-Antonio (Scientific Societies, 2019-02)

[EN] Artificial small RNAs (sRNAs), including artificial microRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs), are used to silence viral RNAs and confer antiviral resistance in plants. ...
Fine-tune control of targeted RNAi efficacy by plant artificial small RNAs

López-Dolz, Lucio; Spada, Maria; Daròs, José-Antonio; CARBONELL, ALBERTO (Oxford University Press, 2020-06-19)

[EN] Eukaryotic RNA interference (RNAi) results in gene silencing upon the sequence-specific degradation of target transcripts by complementary small RNAs (sRNAs). In plants, RNAi-based tools have been optimized for high ...
Functional analysis of three Arabidopsis ARGONAUTES using slicer-defective mutants

CARBONELL, ALBERTO; Fahlgren, Noah; García-Ruíz, Hernan; Gilbert, Kerrigan B.; Montgomery, Taiowa A.; Nguyen, Tammy; Cuperus, Josh T.; Carrington, James C. (American Society of Plant Biologists, 2012-09)

[EN] In RNA-directed silencing pathways, ternary complexes result from small RNA-guided ARGONAUTE (AGO) associating with target transcripts. Target transcripts are often silenced through direct cleavage (slicing), ...
Heterologous RNA silencing suppressors from both plant- and animal-infecting viruses support Plum pox virus infection

Maliogka, Varvara I.; Calvo, Maria; CARBONELL, ALBERTO; Garcia, Juan Antonio; Valli, Adrian (Microbiology Society, 2012-07)

[EN] HCPro, the RNA silencing suppressor (RSS) of viruses belonging to the Potyvirus genus in the Potyviridae family, is a multifunctional protein presumably involved in all essential steps of the viral infection cycle. ...
Highly specific gene silencing in a monocot species by artificial microRNAs derived from chimeric MIRNA precursors

CARBONELL, ALBERTO; Fahlgren, Noah; Mitchell, Skyler; Cox, Kevin L., Jr.; Reilly, Kevin C.; Mockler, Todd C.; Carrington, James C. (Blackwell Publishing, 2015-06)

[EN] Artificial microRNAs (amiRNAs) are used for selective gene silencing in plants. However, current methods to produce amiRNA constructs for silencing transcripts in monocot species are not suitable for simple, cost-effective ...
Identification and Characterization of Stress-Responsive TAS3-Derived TasiRNAs in Melon

Cervera-Seco, Luis; Marques, M.C.; Sanz-Carbonell, Alejandro; Márquez-Molins, Joan; CARBONELL, ALBERTO; DAROS ARNAU, JOSE ANTONIO; Gomez, Gustavo Germán (Oxford University Press, 2019-11)

[EN] Small interfering RNAs (siRNA) are key regulators of gene expression that play essential roles in diverse biological processes. Trans-acting siRNAs (tasiRNAs) are a class of plant-endogenous siRNAs that lead the ...